91a6df96ceab8597ae5c55fa64c9b7a6 pmc_10146001.pdf 22b69d3a757f4e49ec0ff1ad547373b4222d6075 pmc_10146001.pdf 16e6ba1f5bca5286b5a4ad6911fbf8d37b5b583993016653c17a61239c812bf0 pmc_10146001.pdf Title: Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV—Study 2 Subject: This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 105 plaque-forming units (PFU) of live attenuated ASFV strain BA71CD2 and, three weeks later, left in direct contact with pigs infected with the pandemic Georgia 2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5 C before day 20 pe, and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe, and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFN[scale=1]1.pdf-secreting T cells in pigs fed with SDPP soon after the Georgia 2007/01 encounter, confirmed the relevance of Th1-like responses in ASF protection. We believe that our result shows that nutritional interventions might contribute to improving future ASF vaccination strategies. Keywords: African swine fever; ASFV; spray-dried porcine plasma; vaccine; challenge; nutritional intervention Author: Joan Pujols, Elena Blázquez, Joaquim Segalés, Fernando Rodríguez, Chia-Yu Chang, Jordi Argilaguet, Laia Bosch-Camós, Rosa Rosell, Lola Pailler-García, Boris Gavrilov, Joy Campbell and Javier Polo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Apr 11 05:58:03 2023 CEST ModDate: Mon Apr 17 10:15:38 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3280328 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 IYDCBB+VnURWPalladioL Type 1 Custom yes yes no 15 0 LIUTOW+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 JUHCSO+URWPalladioL-Roma Type 1 Custom yes yes no 24 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BPKINN+PalatinoLinotype CID TrueType Identity-H yes yes yes 253 0 BPKINO+PalatinoLinotype TrueType WinAnsi yes yes no 259 0 BPKIPA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 262 0 BPKKHI+PalatinoLinotype TrueType MacRoman yes yes no 265 0 BPKINM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 279 0 BPKINN+PalatinoLinotype CID TrueType Identity-H yes yes yes 282 0 BPKINO+PalatinoLinotype TrueType WinAnsi yes yes no 288 0 BPKIPA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 291 0 BPKKHI+PalatinoLinotype TrueType MacRoman yes yes no 294 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-23 02:34:35 CEST RepresentationInformation: pmc_10146001.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-05-05 02:20:17 CEST Size: 3280328 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 490 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Clinical Monitoring Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Laboratory Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Info: Title: Feeding Spray-Dried Porcine Plasma to Pigs Improves the Protection Afforded by the African Swine Fever Virus (ASFV) BA71CD2 Vaccine Prototype against Experimental Challenge with the Pandemic ASFV Study 2 Author: Joan Pujols, Elena Blázquez, Joaquim Segalés, Fernando Rodríguez, Chia-Yu Chang, Jordi Argilaguet, Laia Bosch-Camós, Rosa Rosell, Lola Pailler-García, Boris Gavrilov, Joy Campbell and Javier Polo Subject: This study aimed to evaluate the effects of feeding spray-dried porcine plasma (SDPP) on the protection afforded by the BA71CD2 African swine fever virus (ASFV) vaccine prototype. Two groups of pigs acclimated to diets without or with 8% SDPP were intranasally inoculated with 105 plaque-forming units (PFU) of live attenuated ASFV strain BA71CD2 and, three weeks later, left in direct contact with pigs infected with the pandemic Georgia 2007/01 ASFV strain. During the post-exposure (pe) period, 2/6 from the conventional diet group showed a transient peak rectal temperature >40.5 C before day 20 pe, and some tissue samples collected at 20 d pe from 5/6 were PCR+ for ASFV, albeit showing Ct values much higher than Trojan pigs. Interestingly, the SDPP group did not show fever, neither PCR+ in blood nor rectal swab at any time pe, and none of the postmortem collected tissue samples were PCR+ for ASFV. Differential serum cytokine profiles among groups at vaccination, and a higher number of ASFV-specific IFN[scale=1]1.pdf-secreting T cells in pigs fed with SDPP soon after the Georgia 2007/01 encounter, confirmed the relevance of Th1-like responses in ASF protection. We believe that our result shows that nutritional interventions might contribute to improving future ASF vaccination strategies. 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