64759370a9a96349def58ed5a7543d0a pmc_10179429.pdf 3a9f111c800e1713111b3339497cbe66777252df pmc_10179429.pdf 1039ab540f24bb8ac001ae1699f06724dd55f05557f7a47a65a480cf30cec324 pmc_10179429.pdf Title: A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum Subject: Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases.Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some cells. Several studies have reported altered protein and RNA levels in different brain areas in schizophrenia. One of these studies found reduced protein levels of METTL7A in the cerebellar cortex in schizophrenia and stress murine models. Since there is limited information in the literature about METTL7A, we characterized its cellular and subcellular localizations in the human cerebellum using immunohistochemical analysis with laser confocal microscopy. Our study reveals a novel METTL7A localization in GFAP-positive cells, with higher expression in the end-feet of the Bergmann glia, which participate in the cerebrospinal fluid–brain parenchyma barrier. Further 3D reconstruction image analysis showed that METTL7A was expressed in the contacts between the Bergmann glia and Purkinje neurons. METTL7A was also detected in lipid droplets in some cells in the white matter. The localization of METTL7A in the human cerebellar glia limitans could suggest a putative role in maintaining the cerebellar parenchyma homeostasis and in the regulation of internal cerebellar circuits by modulating the synaptic activity of Purkinje neurons. Keywords: METTL7A; schizophrenia; postmortem cerebellum; Bergmann glia; glia limitans; cerebrospinal fluid-brain barrier Author: América Vera-Montecinos, Jordi Galiano-Landeira, Mònica Roldán, Francisco Vidal-Domènech, Enrique Claro and Belén Ramos Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sun May 7 11:27:17 2023 CEST ModDate: Mon May 8 03:32:00 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 10422877 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UZLFPZ+VnURWPalladioL Type 1 Custom yes yes no 10 0 SECGJF+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 GCNWCM+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 YKZAYZ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 RGUFCP+CMSY10 Type 1 Builtin yes yes no 28 0 VJOTAC+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 50 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 53 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 78 0 PXEKZD+CMBSY10 Type 1 Builtin yes yes no 82 0 CIHJHI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 93 0 CIHJHK+PalatinoLinotype TrueType WinAnsi yes yes no 96 0 CIHJHM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 99 0 CIHJHI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 117 0 CIHJHJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 120 0 CIHJHK+PalatinoLinotype TrueType WinAnsi yes yes no 126 0 CIHJHM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 129 0 CIHOAD+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 132 0 CIHJHI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 146 0 CIHJHJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 149 0 CIHJHK+PalatinoLinotype TrueType WinAnsi yes yes no 155 0 CIHJHM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 158 0 CIHKDJ+PalatinoLinotype TrueType MacRoman yes yes no 161 0 CIHOAD+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 164 0 CIHJHI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 179 0 CIHJHJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 182 0 CIHJHK+PalatinoLinotype TrueType WinAnsi yes yes no 188 0 CIHJHM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 191 0 CIHOAD+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 194 0 CIHJHI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 211 0 CIHJHJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 214 0 CIHJHK+PalatinoLinotype TrueType WinAnsi yes yes no 220 0 CIHJHM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 223 0 CIHKDJ+PalatinoLinotype TrueType MacRoman yes yes no 226 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-13 02:33:33 CET RepresentationInformation: pmc_10179429.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-07-28 02:23:29 CEST Size: 10422877 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 448 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Characterization of METTL7A in Human Cerebellar Tissue Destination: subsection.2.1 Item: Title: Co-Immunostaining of METTL7A and GFAP in Human Cerebellar Tissue Destination: subsection.2.2 Item: Title: Co-Immunostaining of METTL7A and Tuj1 in Human Cerebellar Tissue Destination: subsection.2.3 Item: Title: Three-Dimensional Reconstruction Analysis Destination: subsection.2.4 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Postmortem Human Brain Tissue Destination: subsection.4.1 Item: Title: Immunohistochemistry of Human Cerebellar Tissue Destination: subsection.4.2 Item: Title: Confocal Microscopy Destination: subsection.4.3 Item: Title: Quantitative Colocalization Destination: subsection.4.4 Item: Title: References Destination: section.A. Info: Title: A Novel Localization of METTL7A in Bergmann Glial Cells in Human Cerebellum Author: América Vera-Montecinos, Jordi Galiano-Landeira, Mònica Roldán, Francisco Vidal-Domènech, Enrique Claro and Belén Ramos Subject: Methyltransferase-like protein 7A (METTL7A) is a member of the METTL family of methyltransferases.Little information is available regarding the cellular expression of METTL7A in the brain. METTL7A is commonly located in the endoplasmic reticulum and to a lesser extent, in the lipid droplets of some cells. Several studies have reported altered protein and RNA levels in different brain areas in schizophrenia. One of these studies found reduced protein levels of METTL7A in the cerebellar cortex in schizophrenia and stress murine models. Since there is limited information in the literature about METTL7A, we characterized its cellular and subcellular localizations in the human cerebellum using immunohistochemical analysis with laser confocal microscopy. Our study reveals a novel METTL7A localization in GFAP-positive cells, with higher expression in the end-feet of the Bergmann glia, which participate in the cerebrospinal fluid brain parenchyma barrier. Further 3D reconstruction image analysis showed that METTL7A was expressed in the contacts between the Bergmann glia and Purkinje neurons. METTL7A was also detected in lipid droplets in some cells in the white matter. The localization of METTL7A in the human cerebellar glia limitans could suggest a putative role in maintaining the cerebellar parenchyma homeostasis and in the regulation of internal cerebellar circuits by modulating the synaptic activity of Purkinje neurons. Keywords: METTL7A; schizophrenia; postmortem cerebellum; Bergmann glia; glia limitans; cerebrospinal fluid-brain barrier Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sun May 07 11:27:17 CEST 2023 ModDate: Mon May 08 03:32:00 CEST 2023 ID: 0x410fd641adfcf6ebbe1ac7ad3676aa6d, 0x410fd641adfcf6ebbe1ac7ad3676aa6d Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: VJOTAC+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 225 FontDescriptor: FontName: VJOTAC+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PXEKZD+CMBSY10 FontSubset: true FirstChar: 48 LastChar: 48 FontDescriptor: FontName: PXEKZD+CMBSY10 Flags: Symbolic FontBBox: -26, -940, 1332, 825 FontFile: true Font: BaseFont: GCNWCM+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 248 FontDescriptor: FontName: GCNWCM+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: 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