111d4b31954db4f23ec2ec4fc8e292b2 pmc_10215467.pdf 2242c6aa9c51ad4c3771d66f5cf6d3d2d55d3496 pmc_10215467.pdf 243828bd7ab5e7e14399a036282a447ea1b117aa0a48478fbb1249a1048f9b9d pmc_10215467.pdf Title: Effects of Antioxidants in Fermented Beverages in Tissue Transcriptomics: Effect of Beer Intake on Myocardial Tissue after Oxidative Injury Subject: Fermented beverages, such as wine and beer, are rich in polyphenols that have been shown to have protective effects against oxidative stress. Oxidative stress plays a central role in the pathogenesis and progression of cardiovascular disease. However, the potential benefits of fermented beverages on cardiovascular health need to be fully investigated at a molecular level. In this study, we aimed at analyzing the effects of beer consumption in modulating the transcriptomic response of the heart to an oxidative stress challenge induced by myocardial ischemia (MI) in the presence of hypercholesterolemia in a pre-clinical swine model. Previous studies have shown that the same intervention induces organ protective benefits. We report a dose-dependent up-regulation of electron transport chain members and the down-regulation of spliceosome-associated genes linked to beer consumption. Additionally, low-dose beer consumption resulted in a down-regulation of genes associated with the immune response, that was not shown for moderate-dose beer consumption. These findings, observed in animals having demonstrated beneficial effects at the organ-level, indicate that the antioxidants in beer differentially affect the myocardial transcriptome in a dose-dependent manner. Keywords: myocardial infarction; hypercholesterolemia; beer; alcohol; polyphenols Author: Alex Gallinat, Gemma Vilahur, Teresa Padro and Lina Badimon Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat May 13 10:13:48 2023 CEST ModDate: Sat May 13 11:04:50 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5232450 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BMWNIW+VnURWPalladioL Type 1 Custom yes yes no 10 0 GWNBUY+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 HWBNZK+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 KLIEHR+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 42 0 WTPQBM+CMSY10 Type 1 Builtin yes yes no 64 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 71 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 85 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 88 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 91 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 97 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 100 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 106 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 121 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 124 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 127 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 133 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 136 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 157 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 160 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 163 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 169 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 172 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 178 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 193 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 196 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 199 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 205 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 208 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 214 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 226 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 229 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 232 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 238 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 241 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 247 0 PJKKMC+PalatinoLinotype TrueType WinAnsi yes yes no 264 0 PJKKAO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 267 0 PJKKAP+TimesNewRoman CID TrueType Identity-H yes yes yes 270 0 PJKKBB+TimesNewRoman,Italic TrueType WinAnsi yes yes no 276 0 PJKKBC+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 279 0 PJKKLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 285 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-03 02:40:52 CEST RepresentationInformation: pmc_10215467.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-04-24 02:41:04 CEST Size: 5232450 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 468 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Experimental Design Destination: subsection.2.1 Item: Title: Animal Model of MI Destination: subsection.2.2 Item: Title: Sample Collection Destination: subsection.2.3 Item: Title: Sample Processing Destination: subsection.2.4 Item: Title: Transcriptomics and In Silico Analysis Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Beer Intake Modifies the Global Gene Expression Profile of the Myocardium Destination: subsection.3.1 Item: Title: Beer Consumption Up-Regulates Electron Transport Chain Members and Down-Regulates Spliceosome-Associated Genes Destination: subsection.3.2 Item: Title: Low and Moderate-Dose Beer Regimes Have Opposite Effects upon Post-MI Immune Response Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.A. Info: Title: Effects of Antioxidants in Fermented Beverages in Tissue Transcriptomics: Effect of Beer Intake on Myocardial Tissue after Oxidative Injury Author: Alex Gallinat, Gemma Vilahur, Teresa Padro and Lina Badimon Subject: Fermented beverages, such as wine and beer, are rich in polyphenols that have been shown to have protective effects against oxidative stress. Oxidative stress plays a central role in the pathogenesis and progression of cardiovascular disease. However, the potential benefits of fermented beverages on cardiovascular health need to be fully investigated at a molecular level. In this study, we aimed at analyzing the effects of beer consumption in modulating the transcriptomic response of the heart to an oxidative stress challenge induced by myocardial ischemia (MI) in the presence of hypercholesterolemia in a pre-clinical swine model. Previous studies have shown that the same intervention induces organ protective benefits. We report a dose-dependent up-regulation of electron transport chain members and the down-regulation of spliceosome-associated genes linked to beer consumption. Additionally, low-dose beer consumption resulted in a down-regulation of genes associated with the immune response, that was not shown for moderate-dose beer consumption. These findings, observed in animals having demonstrated beneficial effects at the organ-level, indicate that the antioxidants in beer differentially affect the myocardial transcriptome in a dose-dependent manner. 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