c5c2ffdf5f2278e7bc680376419e65c2 pmc_10215614.pdf 62d501bc73be506a772a3f14ccc2f733c79509a1 pmc_10215614.pdf 37e643f991493a1d696f15a270b6f1fb61b43c4708e32124d2ffa33f2cc16ede pmc_10215614.pdf Title: Signalment, Immunological and Parasitological Status and Clinicopathological Findings of Leishmania-Seropositive Apparently Healthy Dogs Subject: Canine leishmaniosis caused by Leishmania infantum is a disease with a wide range of clinical manifestations. Epidemiological serosurveys performed in Europe often lack a thorough assessment of clinical health status of studied dogs. The aim of this study was to evaluate signalment, immunological and parasitological status and clinicopathological findings of L. infantum-seropositive apparently healthy dogs (n = 212) living in endemic areas. Routine laboratory tests, endpoint in-house ELISA to quantify the anti-Leishmania antibodies, blood Leishmania qPCR and IFN- ELISA were performed. All dogs enrolled were L. infantum-seropositive and were classified as healthy (n = 105) or sick (n = 107) according to LeishVet guidelines. The sick group presented a higher proportion of medium to high antibody levels and positive qPCR and lower IFN- concentration compared to the healthy group. Sick dogs were mostly classified in LeishVet stage IIa. Biochemical alterations (98%) were the most common clinicopathological findings, with fewer urinary tract (46%) and hematological (40%) alterations. Apparently healthy L. infantum-seropositive dogs can be classified between truly healthy dogs and sick dogs with clinicopathological findings. Sick dogs presented medium to high seropositivity and parasitemia and low IFN- concentrations, and their most common clinicopathological abnormalities were serum protein alterations followed by proteinuria and lymphopenia.+16pt Keywords: antibody level; canine leishmaniosis; ELISA; IFN-; Leishmania infantum; quantitative PCR Author: Marta Baxarias, Oriol Jornet-Rius, Giulia Donato, Cristina Mateu, Mª Magdalena Alcover, Maria Grazia Pennisi and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue May 16 10:14:33 2023 CEST ModDate: Tue May 16 10:35:51 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 390080 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EKDTZH+VnURWPalladioL Type 1 Custom yes yes no 10 0 RDIJYX+EURM10 Type 1 Builtin yes yes no 15 0 BHQDFN+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 JLWZGO+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 IYBCJU+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 ENLSQP+URWPalladioL-BoldItal Type 1 Custom yes yes no 32 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 VORBJF+CMSY10 Type 1 Builtin yes yes no 75 0 GEWEQC+PazoMath-Italic Type 1 Builtin yes yes no 82 0 RUSYBV+EURB10 Type 1 Builtin yes yes no 92 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-06-30 02:35:38 CEST RepresentationInformation: pmc_10215614.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-06-22 02:22:41 CEST Size: 390080 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 497 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Dogs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Quantitative ELISA for the Detection of L. infantum-Specific Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Blood DNA Extraction and Leishmania quantitative PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: IFN- Release Whole Blood Stimulation Assay (WBA) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: Signalment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Clinical Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Clinicopathological Findings in Seropositive Sick Dogs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Relationship between Clinicopathological Findings, Signalment, Antibody Levels, Leishmania Quantitative PCR, IFN- Concentration and Clinical Staging Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Info: Title: Signalment, Immunological and Parasitological Status and Clinicopathological Findings of Leishmania-Seropositive Apparently Healthy Dogs Author: Marta Baxarias, Oriol Jornet-Rius, Giulia Donato, Cristina Mateu, Mª Magdalena Alcover, Maria Grazia Pennisi and Laia Solano-Gallego Subject: Canine leishmaniosis caused by Leishmania infantum is a disease with a wide range of clinical manifestations. Epidemiological serosurveys performed in Europe often lack a thorough assessment of clinical health status of studied dogs. The aim of this study was to evaluate signalment, immunological and parasitological status and clinicopathological findings of L. infantum-seropositive apparently healthy dogs (n = 212) living in endemic areas. Routine laboratory tests, endpoint in-house ELISA to quantify the anti-Leishmania antibodies, blood Leishmania qPCR and IFN- ELISA were performed. All dogs enrolled were L. infantum-seropositive and were classified as healthy (n = 105) or sick (n = 107) according to LeishVet guidelines. The sick group presented a higher proportion of medium to high antibody levels and positive qPCR and lower IFN- concentration compared to the healthy group. Sick dogs were mostly classified in LeishVet stage IIa. Biochemical alterations (98%) were the most common clinicopathological findings, with fewer urinary tract (46%) and hematological (40%) alterations. Apparently healthy L. infantum-seropositive dogs can be classified between truly healthy dogs and sick dogs with clinicopathological findings. 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