1e21bb1bb02e806e5b420ba3d6923cad pmc_10235030.pdf e2c5e5e55ef8b9f9597bd4c8b8064945cc48c6f4 pmc_10235030.pdf f25b0fdf5a9cebabc490310fc3f23f4d6d1ed0abe7fe7cd8c8410a9b451b0cdc pmc_10235030.pdf Title: A de novo evolved domain improves the cadmium detoxification capacity of limpet metallothioneins Subject: Scientific Reports, https://doi.org/10.1038/s41598-023-35786-1 Author: Mario García-Risco Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Thu Jun 1 09:41:50 2023 CEST ModDate: Thu Jun 1 10:51:59 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2884266 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IFLXVX+Wingdings-Regular Type 1C WinAnsi yes yes yes 481 0 MinionPro-It Type 1C WinAnsi yes no yes 46 0 MinionPro-Regular Type 1C Custom yes no yes 45 0 Corbel-BoldItalic TrueType WinAnsi yes no yes 68 0 DHWUFC+Corbel-Bold CID TrueType Identity-H yes yes yes 47 0 Corbel2 TrueType WinAnsi yes no yes 48 0 OKPSDG+Corbel CID TrueType Identity-H yes yes yes 49 0 Corbel3 CID TrueType Identity-H yes no yes 520 0 MinionPro-Bold Type 1C Custom yes no yes 67 0 Corbel4 TrueType WinAnsi yes no yes 335 0 MinionPro-BoldIt Type 1C WinAnsi yes no yes 205 0 AYEFKI+TimesNewRomanPSMT TrueType WinAnsi yes yes yes 247 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-02-02 02:34:12 CET RepresentationInformation: pmc_10235030.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-12-14 02:19:47 CET Size: 2884266 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 550 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: A de novo evolved domain improves the cadmium detoxification capacity of limpet metallothioneins Children: Item: Title: Materials and methods Children: Item: Title: Production and purification of recombinant metal-MT complexes. Item: Title: Metal determination and protein quantification of LgiMT1, LgiMT2 and γLgiMT2 preparations. Item: Title: Metal-LgiMT1, LgiMT2 and γLgiMT2 species determination. Item: Title: Spectroscopic characterization (CD and UV–Vis). Item: Title: Results and discussion Children: Item: Title: Recombinant production of metal-MT complexes of Lottia gigantea metallothioneins. Item: Title: LgiMT1 and LgiMT2 bind divalent metal ions with a high preference for Cd(II). Item: Title: Characterization of the novel γ domain with a suggested adamantane structure. Item: Title: The new Patellogastropoda γ domain: an evolutionary resounding and ecologically significant innovation. 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Reports, https://doi.org/10.1038/s41598-023-35786-1 A de novo evolved domain improves the cadmium detoxification capacity of limpet metallothioneins Mario García-Risco Sara Calatayud Veronika Pedrini-Martha Ricard Albalat Òscar Palacios Mercè Capdevila Reinhard Dallinger 10.1038/s41598-023-35786-1 2010-04-23 true springer.com springerlink.com https://doi.org/10.1038/s41598-023-35786-1 10.1038/s41598-023-35786-1 2045-2322 journal Scientific Reports The Author(s) 2010-04-23 true 10.1038/s41598-023-35786-1 noindex springer.com springerlink.com VoR uuid:d70254cb-01fd-4962-a808-e3de76cff25d uuid:43c86ba0-65c9-49be-ad71-969543faa777 default 1 converted uuid:21fe91fb-e0c0-46ed-affb-d2578f75b7a8 converted to PDF/A-2b pdfToolbox 2023-06-01T13:13:17+05:30 converted uuid:8fdb8109-d347-4bd4-bb96-88ebf718a2e7 converted to PDF/A-2b pdfToolbox 2023-06-01T13:15:03+05:30 Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) 2 B 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