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Psychiatry 14:1148993. doi: 10.3389/fpsyt.2023.1148993 Keywords: cannabis addiction, adolescence, WIN 55,212-2 self-administration mouse model, THC, impulsivity, drd2, adora2a Author: María del Mar Cajiao-Manrique Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.16 CreationDate: Fri May 19 13:13:18 2023 CEST ModDate: Fri May 19 13:56:41 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6115697 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JXEWUX+MinionPro-Regular Type 1 Custom yes yes no 27 0 BVGXBB+MuseoSans-700 Type 1 Custom yes yes no 28 0 EMMHCK+MuseoSans-300 Type 1 Custom yes yes no 29 0 XIZUJH+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 34 0 VUXKVF+MuseoSans-300Italic Type 1 Custom yes yes no 35 0 TZBIOG+MuseoSans-500 Type 1 Custom yes yes no 36 0 MUGGMO+RBLMI Type 1 Builtin yes yes no 42 0 EWYLZG+MTGU Type 1 Builtin yes yes no 43 0 GLAVUJ+MuseoSans-700Italic Type 1 Custom yes yes no 44 0 RDOKFH+MinionPro-It Type 1 Custom yes yes no 107 0 OJWPOY+MTSYN Type 1 Builtin yes yes no 114 0 XABZJU+MinionPro-Bold Type 1 Custom yes yes no 159 0 CHHPJQ+MuseoSans-500Italic Type 1 Custom yes yes no 503 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-10-05 02:29:28 CEST RepresentationInformation: pmc_10248087.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-10-04 02:23:18 CEST Size: 6115697 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 1138 FreeObjects: 13 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: THC exposure during adolescence increases impulsivity-like behavior in adulthood in a WIN 55,212-2 self-administration mouse model Children: Item: Title: 1. Introduction Item: Title: 2. Materials and methods Children: Item: Title: 2.1. Animals Item: Title: 2.2. Drugs Item: Title: 2.3. Operant self-administration apparatus Item: Title: 2.4. Experimental design Item: Title: 2.5. Adolescent THC treatment Item: Title: 2.6. WIN 55,212-2 self-administration Children: Item: Title: 2.6.1. Jugular vein catheterization Item: Title: 2.6.2. WIN 55,212-2 self-administration training Item: Title: 2.6.3. Three addiction-like criteria Children: Item: Title: 2.6.3.1. Persistence of response Item: Title: 2.6.3.2. Motivation Item: Title: 2.6.3.3. Compulsivity Item: Title: 2.6.4. Establishment of mice subpopulations Item: Title: 2.6.5. Extinction and parameters related to craving Children: Item: Title: 2.6.5.1. Resistance to extinction Item: Title: 2.6.5.2. Drug-seeking behavior measured by cue-induced reinstatement of WIN 55,212-2-seeking behavior Item: Title: 2.6.6. Behavioral tests to evaluate addiction-like phenotypic traits Children: Item: Title: 2.6.6.1. Impulsivity Item: Title: 2.6.6.2. Sensitivity to reward Item: Title: 2.7. Gene expression analysis Children: Item: Title: 2.7.1. Tissue preparation Item: Title: 2.7.2. RNA extraction Item: Title: 2.7.3. Quantitative polymerase chain reaction (qPCR) Item: Title: 2.8. Statistics Children: Item: Title: 2.8.1. Statistical analysis of behavioral data Item: Title: 2.8.2. Principal component analysis Item: Title: 3. Results Children: Item: Title: 3.1. Effects of THC pre-treatment during adolescence on the operant model of cannabinoid addiction-like behavior using WIN 55,212-2 self-administration training in adulthood Item: Title: 3.2. Mouse model of addiction-like behavior using WIN 55,212-2 self-administration and THC pre-treatment during adolescence Item: Title: 3.3. THC pre-exposure during adolescence decreased the resistance to extinction in adulthood Item: Title: 3.4. THC exposure during adolescence increased impulsivity-like behavior in adulthood Item: Title: 3.5. High- and low-impulsive subgroups reveal behavioral differences in adult mice after WIN 55,212-2 self-administration Item: Title: 3.6. THC pre-treatment did not produce major long-term behavioral or somatic alterations Item: Title: 3.7. Principal component analysis of THC exposure during adolescence in self-administration of WIN 55,212-2 in adulthood Item: Title: 3.8. Correlation heatmap between the addiction-like criteria, parameters related to craving and vulnerability phenotypic traits Item: Title: 3.9. THC exposure during adolescence produced a downregulation of the drd2 and adora2a gene expression in the NAc and HPC Item: Title: 4. Discussion Item: Title: 5. Conclusion Item: Title: Data availability statement Item: Title: Ethics statement Item: Title: Author contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Conflict of interest Item: Title: Publisher's note Item: Title: Supplementary material Item: Title: References Info: Title: THC exposure during adolescence increases impulsivity-like behavior in adulthood in a WIN 55,212-2 self-administration mouse model Author: María del Mar Cajiao-Manrique Subject: Cajiao-Manrique MdM, Casadó-Anguera V, García-Blanco A, Maldonado R and Martín-García E (2023) THC exposure during adolescence increases impulsivity-like behavior in adulthood in a WIN 55,212-2 self-administration mouse model. Front. Psychiatry 14:1148993. doi: 10.3389/fpsyt.2023.1148993 Keywords: cannabis addiction, adolescence, WIN 55,212-2 self-administration mouse model, THC, impulsivity, drd2, adora2a Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.16 CreationDate: Fri May 19 13:43:18 CEST 2023 ModDate: Fri May 19 14:26:41 CEST 2023 ID: 0xdf7b53acba7ef1cc7cf0af226c5e8183, 0xd80f6a0963e29741bb15ff56ff0e6293 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: XIZUJH+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 134 LastChar: 134 FontDescriptor: FontName: XIZUJH+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: VUXKVF+MuseoSans-300Italic FontSubset: true FirstChar: 44 LastChar: 121 FontDescriptor: FontName: VUXKVF+MuseoSans-300Italic Flags: Symbolic FontBBox: -245, -210, 1030, 883 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TZBIOG+MuseoSans-500 FontSubset: true FirstChar: 3 LastChar: 243 FontDescriptor: FontName: TZBIOG+MuseoSans-500 Flags: Symbolic FontBBox: -121, -210, 1088, 892 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MUGGMO+RBLMI FontSubset: true FirstChar: 46 LastChar: 62 FontDescriptor: FontName: MUGGMO+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: EWYLZG+MTGU FontSubset: true FirstChar: 98 LastChar: 109 FontDescriptor: FontName: EWYLZG+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: RDOKFH+MinionPro-It FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: RDOKFH+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GLAVUJ+MuseoSans-700Italic FontSubset: true FirstChar: 50 LastChar: 114 FontDescriptor: FontName: GLAVUJ+MuseoSans-700Italic Flags: Symbolic FontBBox: -254, -210, 1112, 902 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OJWPOY+MTSYN FontSubset: true FirstChar: 0 LastChar: 67 FontDescriptor: FontName: OJWPOY+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: CHHPJQ+MuseoSans-500Italic FontSubset: true FirstChar: 50 LastChar: 114 FontDescriptor: FontName: CHHPJQ+MuseoSans-500Italic Flags: Symbolic FontBBox: -249, -210, 1047, 892 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: JXEWUX+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: JXEWUX+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: BVGXBB+MuseoSans-700 FontSubset: true FirstChar: 40 LastChar: 150 FontDescriptor: FontName: BVGXBB+MuseoSans-700 Flags: Symbolic FontBBox: -125, -210, 1126, 902 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EMMHCK+MuseoSans-300 FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: EMMHCK+MuseoSans-300 Flags: Symbolic FontBBox: -118, -210, 1088, 883 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XABZJU+MinionPro-Bold FontSubset: true FirstChar: 44 LastChar: 150 FontDescriptor: FontName: XABZJU+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true XMP: cannabis addiction, adolescence, WIN 55,212-2 self-administration mouse model, THC, impulsivity, drd2, adora2a pdfTeX-1.40.16 1.5 True https://www.frontiersin.org/legal/copyright-statement application/pdf fpsyt-14-1148993.tex THC exposure during adolescence increases impulsivity-like behavior in adulthood in a WIN 55,212-2 self-administration mouse model Cajiao-Manrique MdM, Casadó-Anguera V, García-Blanco A, Maldonado R and Martín-García E (2023) THC exposure during adolescence increases impulsivity-like behavior in adulthood in a WIN 55,212-2 self-administration mouse model. Front. Psychiatry 14:1148993. doi: 10.3389/fpsyt.2023.1148993 Copyright © 2023 Cajiao-Manrique, Casadó-Anguera, García-Blanco, Maldonado and Martín-García. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). María del Mar Cajiao-Manrique Verònica Casadó-Anguera Alejandra García-Blanco Rafael Maldonado Elena Martín-García 2023-05-19T16:43:18+05:30 cannabis addiction adolescence WIN 55 212-2 self-administration mouse model THC impulsivity drd2 adora2a 2023-05-19T16:43:18+05:30 2023-05-19T17:26:41+05:30 2023-05-19T17:26:41+05:30 LaTeX with hyperref package uuid:626262b1-8d75-4467-b205-900a8dc900a8 uuid:b294dc35-6b5a-46c3-af8f-41746feaf6ba Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 48, 791, 222, 807 Annotation: Subtype: Link Rect: 433, 798, 514, 807 Annotation: Subtype: Link Rect: 433, 789, 517, 798 Annotation: Subtype: Link Rect: 433, 779, 545, 789 Annotation: Subtype: Link Rect: 48, 704, 126, 721 Annotation: Subtype: Link Rect: 62, 400, 154, 409 Annotation: Subtype: Link Rect: 101, 352, 129, 359 Annotation: Subtype: Link Rect: 129, 352, 165, 359 Annotation: Subtype: Link Rect: 165, 352, 201, 359 Annotation: Subtype: Link Rect: 48, 342, 74, 350 Annotation: Subtype: 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