52b85bfbb9c7f3141f90c43788b9450b pmc_10253750.pdf 9bdb6a38858ffda0fa255a5dff65143729c9e8f7 pmc_10253750.pdf 99ba93dc6b9447bfac45c027da29e27fd6c5d5b632b8ce480e053dd200d782a1 pmc_10253750.pdf Title: Changes of Erythrocyte Fatty Acids after Supplementation with Highly Concentrated Docosahexaenoic Acid (DHA) in Pediatric Cystic Fibrosis: A Randomized Double-Blind Controlled Trial Subject: We characterized the fatty acid profiles in the erythrocyte membrane of pediatric patients with cystic fibrosis (CF) receiving highly concentrated docosahexaenoic acid (DHA) supplementation (Tridocosahexanoin-AOX® 70%) at 50 mg/kg/day (n = 11) or matching placebo (n = 11) for 12 months. The mean age was 11.7 years. The DHA group showed a statistically significant improvement in n-3 polyunsaturated fatty acids (PUFAs), which was observed as early as 6 months and further increased at 12 months. Among the n-3 PUFAs, there was a significant increase in DHA and eicosapentaenoic acid (EPA). Additionally, a statistically significant decrease in n-6 PUFAs was found, primarily due to a decrease in arachidonic acid (AA) levels and elongase 5 activity. However, we did not observe any changes in linoleic acid levels. The long-term administration of DHA over one year was safe and well tolerated. In summary, the administration of a high-rich DHA supplement at a dose of 50 mg/kg/day for one year can correct erythrocyte AA/DHA imbalance and reduce fatty acid inflammatory markers. However, it is important to note that essential fatty acid alterations cannot be fully normalized with this treatment. These data provide timely information of essential fatty acid profile for future comparative research. Keywords: cystic fibrosis; fatty acids; docosahexaenoic acid; eicosapentaenoic acid; omega-3; pancreatic insufficiency; randomized controlled study Author: Roser Ayats-Vidal, Montserrat Bosque-García, Begoña Cordobilla, Oscar Asensio-De la Cruz, Miguel García-González, Jesús Castro-Marrero, Irene López-Rico and Joan Carles Domingo Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon May 29 12:48:27 2023 CEST ModDate: Mon May 29 12:55:01 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 988550 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TBJWQS+VnURWPalladioL Type 1 Custom yes yes no 10 0 CKRMGR+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 YITBNG+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 TTTEWI+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 JCYIDT+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 49 0 MDJLZU+EURM10 Type 1 Builtin yes yes no 71 0 UWJUYN+CMSY10 Type 1 Builtin yes yes no 78 0 KSGHMQ+PazoMath Type 1 Builtin yes yes no 85 0 GEWEQC+PazoMath-Italic Type 1 Builtin yes yes no 89 0 JFOAAE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 103 0 JFOAAF+PalatinoLinotype CID TrueType Identity-H yes yes yes 106 0 JFOAAG+PalatinoLinotype TrueType WinAnsi yes yes no 112 0 JFOAAI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 115 0 JFOBDC+PalatinoLinotype TrueType MacRoman yes yes no 118 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 125 0 JFOAAE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 173 0 JFOAAF+PalatinoLinotype CID TrueType Identity-H yes yes yes 176 0 JFOAAG+PalatinoLinotype TrueType WinAnsi yes yes no 182 0 JFOAAI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 185 0 JFOBDC+PalatinoLinotype TrueType MacRoman yes yes no 188 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-03-26 02:35:23 CET RepresentationInformation: pmc_10253750.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-03-15 02:38:13 CET Size: 988550 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 373 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Design and Participants Destination: subsection.2.1 Item: Title: Intervention and Study Procedures Destination: subsection.2.2 Item: Title: Blood Samples and Analysis of Fatty Acids Destination: subsection.2.3 Item: Title: Panel of Fatty Acids Destination: subsection.2.4 Item: Title: Study Outcomes Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Baseline Fatty Acid Profile Destination: subsection.3.1 Item: Title: Changes of Fatty Acid Profile in the DHA and Placebo Groups Destination: subsection.3.2 Item: Title: Safety and Adherence Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Changes of Erythrocyte Fatty Acids after Supplementation with Highly Concentrated Docosahexaenoic Acid (DHA) in Pediatric Cystic Fibrosis: A Randomized Double-Blind Controlled Trial Author: Roser Ayats-Vidal, Montserrat Bosque-García, Begoña Cordobilla, Oscar Asensio-De la Cruz, Miguel García-González, Jesús Castro-Marrero, Irene López-Rico and Joan Carles Domingo Subject: We characterized the fatty acid profiles in the erythrocyte membrane of pediatric patients with cystic fibrosis (CF) receiving highly concentrated docosahexaenoic acid (DHA) supplementation (Tridocosahexanoin-AOX® 70%) at 50 mg/kg/day (n = 11) or matching placebo (n = 11) for 12 months. The mean age was 11.7 years. The DHA group showed a statistically significant improvement in n-3 polyunsaturated fatty acids (PUFAs), which was observed as early as 6 months and further increased at 12 months. Among the n-3 PUFAs, there was a significant increase in DHA and eicosapentaenoic acid (EPA). Additionally, a statistically significant decrease in n-6 PUFAs was found, primarily due to a decrease in arachidonic acid (AA) levels and elongase 5 activity. However, we did not observe any changes in linoleic acid levels. The long-term administration of DHA over one year was safe and well tolerated. In summary, the administration of a high-rich DHA supplement at a dose of 50 mg/kg/day for one year can correct erythrocyte AA/DHA imbalance and reduce fatty acid inflammatory markers. However, it is important to note that essential fatty acid alterations cannot be fully normalized with this treatment. These data provide timely information of essential fatty acid profile for future comparative research. 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