a2ae93481a03c4cb5346b902714b69d5 pmc_10298310.pdf 7ac9185a631bd2a6d22bf7187d7e957d1801bc9b pmc_10298310.pdf b9d7ba210a2a0dc148b45d6ac47a449065dada568aac7c85522f2f67705c0274 pmc_10298310.pdf Title: IL-10 Modulates the Expression and Activation of Pattern Recognition Receptors in Mast Cells Subject: Mast cells (MCs) are involved in several immune-related responses, including those in bacterial infections, autoimmune diseases, inflammatory bowel diseases, and cancer, among others. MCs identify microorganisms by pattern recognition receptors (PRRs), activating a secretory response. Interleukin (IL)-10 has been described as an important modulator of MC responses; however, its role in PRR-mediated activation of MC is not fully understood. We analyzed the activation of TLR2, TLR4, TLR7 and Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mucosal-like MCs (MLMCs) and peritoneum-derived cultured MCs (PCMCs) from IL-10-/- and wild-type (WT) mice. IL-10-/- mice showed a reduced expression of TLR4 and NOD2 at week 6 and TLR7 at week 20 in MLMC. In MLMC and PCMC, TLR2 activation induced a reduced secretion of IL-6 and TNF in IL-10-/- MCs. TLR4- and TLR7-mediated secretion of IL-6 and TNF was not detected in PCMCs. Finally, no cytokine release was induced by NOD2 ligand, and responses to TLR2 and TLR4 were lower in MCs at 20 weeks. These findings indicate that PRR activation in MCs depends on the phenotype, ligand, age, and IL-10. Keywords: interleukin-10; MMC; PCMC; PRR; NOD2; TLR Author: Roberto Riquelme-Neira, Romina Walker-Vergara, Joan Antoni Fernández-Blanco and Patrocinio Vergara Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jun 8 10:03:13 2023 CEST ModDate: Thu Jun 8 10:06:57 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2480873 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AWHDWW+VnURWPalladioL Type 1 Custom yes yes no 10 0 ZIPDEL+EURM10 Type 1 Builtin yes yes no 15 0 HLXJDE+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 WMHGKA+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 AGFMRF+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 32 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 36 0 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Identity-H yes yes yes 310 0 KKEGOG+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 316 0 KKEGOH+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 319 0 KKEGOI+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 322 0 KKEHGG+PalatinoLinotype-Roman TrueType MacRoman yes yes no 325 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 341 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-09-21 02:28:56 CEST RepresentationInformation: pmc_10298310.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-09-16 02:17:14 CEST Size: 2480873 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 592 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Morphological Analysis and mMCP-1 and mMCP-6 Characterization of MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Effects of IL-10 Deletion on TLR2 Expression and Release of Inflammatory Mediators Related to Its Activation by Pam2CSK4 in MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Effects of IL-10 Deletion on TLR4 Expression and Release of Inflammatory Mediators Related to Its Activation by LPS in MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Effects of IL-10 Deletion on TLR7 Expression and Release of Inflammatory Mediators Related to Its Activation by Imiquimod in MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Effects of IL-10 Deletion on NOD2 Expression and Release of Inflammatory Mediators Related to Its Activation by MDP in MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Effects of the Lack IL-10 on FcRI-Mediated Responses in MLMC and PCMC Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Children: Item: Title: Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Collection and Culture of Peritoneum-Derived Cultured Mast Cells (PCMC) and Mucosal-like Mast Cells (MLMC) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Cytospin and Staining of Mast Cell Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: In Vitro Cell Stimulation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: RNA Extraction and Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Flow Cytometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Quantification of Cytokines Concentration Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Info: Title: IL-10 Modulates the Expression and Activation of Pattern Recognition Receptors in Mast Cells Author: Roberto Riquelme-Neira, Romina Walker-Vergara, Joan Antoni Fernández-Blanco and Patrocinio Vergara Subject: Mast cells (MCs) are involved in several immune-related responses, including those in bacterial infections, autoimmune diseases, inflammatory bowel diseases, and cancer, among others. MCs identify microorganisms by pattern recognition receptors (PRRs), activating a secretory response. Interleukin (IL)-10 has been described as an important modulator of MC responses; however, its role in PRR-mediated activation of MC is not fully understood. We analyzed the activation of TLR2, TLR4, TLR7 and Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) in mucosal-like MCs (MLMCs) and peritoneum-derived cultured MCs (PCMCs) from IL-10-/- and wild-type (WT) mice. IL-10-/- mice showed a reduced expression of TLR4 and NOD2 at week 6 and TLR7 at week 20 in MLMC. In MLMC and PCMC, TLR2 activation induced a reduced secretion of IL-6 and TNF in IL-10-/- MCs. TLR4- and TLR7-mediated secretion of IL-6 and TNF was not detected in PCMCs. Finally, no cytokine release was induced by NOD2 ligand, and responses to TLR2 and TLR4 were lower in MCs at 20 weeks. These findings indicate that PRR activation in MCs depends on the phenotype, ligand, age, and IL-10. 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59, 400, 96, 410 Checksum: 12498d18 Type: CRC32 Checksum: a2ae93481a03c4cb5346b902714b69d5 Type: MD5 Checksum: 7ac9185a631bd2a6d22bf7187d7e957d1801bc9b Type: SHA-1