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application/pdf Profile: Tagged PDF PDFMetadata: Objects: 1797 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux Children: Item: Title: Abstract Item: Title: Background Item: Title: Materials and methods Children: Item: Title: Strains Item: Title: Model generation Item: Title: In silico design of 13C-labelling experiments Item: Title: Media, cultivation conditions, and automated sampling Item: Title: Analyses Children: Item: Title: Exometabolites analysis with NMR Item: Title: Sample processing and analysis of the 13C-proteinogenic amino acids using LC/MS Item: Title: Bioprocess parameters Item: Title: 13C-Flux calculation Item: Title: Flux balance analysis Item: Title: Results and discussion Children: Item: Title: HT cultivation bioprocess parameters Item: Title: Fluxome ofP. pastoris3-HP-producing strains at pH 5 Item: Title: ATP and NADPH producing/consuming fluxes of the 3-HP-producing P. pastoris strains Item: Title: Fluxome of P. pastoris strains at pH 3.5 Item: Title: Conclusions Item: Title: References Info: Creator: Adobe InDesign 18.0 (Windows) Producer: Adobe PDF Library 17.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Fri Jun 23 08:00:41 CEST 2023 ModDate: Thu Jun 29 04:06:27 CEST 2023 ID: 0xffbfbab781b216e8092b90c330edcf2a, 0x751df047917bada78a53e6f78c53f230 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg 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