407248435b2d5d740c120765110f3296 pmc_10376092.pdf f1184fdac19151e16988f709b6ce5007a9d41238 pmc_10376092.pdf b463ed4cfd02accc22bad6b884fc15b50fabf9d43c95b02589b32b636ebd1167 pmc_10376092.pdf Title: Porcine Forebrain Vacuolization Associated with Wasting in Pigs: A Novel Pathological Outcome Associated with Vitamin–Mineral Deficiency? Subject: The term wasting refers to a clinical sign used to describe a physical condition characterized by growth retardation, usually of multifactorial origin. The objective of the present study was to describe for the first time a pathological process characterized by forebrain neuropil vacuolization in pigs showing wasting without conspicuous neurological signs. To characterize the lesions pathologically, affected and non-affected pigs from eight of these farms were investigated. Histologically, the most consistent lesion was neuropil vacuolization of the prosencephalon, mainly located in the thalamic nuclei and in the transition between the white and grey matter of the neocortex (40/56 in sick and 4/30 in healthy pigs). In the most severe cases, the vacuolation also involved the midbrain, cerebellar nuclei and, to a lesser extent, the medulla oblongata. Vacuolization of the forebrain was associated with pigs experiencing marked emaciation and growth retardation. Although the specific cause of the present case remained unknown, the preventive use of multivitamin and mineral complexes in drinking water ameliorated the condition, strongly suggesting a metabolic origin of the observed condition. Keywords: sus scrofa; brain vacuolization; spongiosis; wasting; metabolic disorder Author: E. Ruiz-Riera, E. Vidal, A. Canturri, A. Lehmbecker, M. Cuvertoret, C. Lopez-Figueroa, W. Baumgärtner, M. Domingo and J. Segalés Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jul 10 10:17:40 2023 CEST ModDate: Mon Jul 10 10:43:46 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 6283501 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BTFGED+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 UZLFPZ+VnURWPalladioL Type 1 Custom yes yes no 15 0 GYNXFI+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 IAJNKP+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 VLWSEG+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 NCKHBC+CMSY10 Type 1 Builtin yes yes no 71 0 ECTHDD+VnURWPalladioL-Italic Type 1 Custom yes yes no 81 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 85 0 LDGGJN+PalatinoLinotype,Bold 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LDGGLB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 200 0 LDGIPF+PalatinoLinotype TrueType MacRoman yes yes no 203 0 LDGGJN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 218 0 LDGGJO+PalatinoLinotype CID TrueType Identity-H yes yes yes 221 0 LDGGKP+PalatinoLinotype TrueType WinAnsi yes yes no 227 0 LDGGLB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 230 0 LDGIPF+PalatinoLinotype TrueType MacRoman yes yes no 233 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-09-14 02:28:50 CEST RepresentationInformation: pmc_10376092.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-09-09 02:19:38 CEST Size: 6283501 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 377 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Clinical Presentation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Study Population and Data Collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Sample Collection and Histopathology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Biochemical and Hematological Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Determination of Heavy Metals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Histochemical and Immunohistochemical Studies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Molecular Biology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Transmission Electron Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: Clinical and Gross Findings Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Biochemistry, Hematology and Heavy Metal Determination in Serum Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Pathogen Detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: General Histopathological Findings Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Neuropathological Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Histochemical and Inmunohistochemical Findings of the Brain Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Transmission Electron Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Info: Title: Porcine Forebrain Vacuolization Associated with Wasting in Pigs: A Novel Pathological Outcome Associated with Vitamin Mineral Deficiency? Author: E. Ruiz-Riera, E. Vidal, A. Canturri, A. Lehmbecker, M. Cuvertoret, C. Lopez-Figueroa, W. Baumgärtner, M. Domingo and J. Segalés Subject: The term wasting refers to a clinical sign used to describe a physical condition characterized by growth retardation, usually of multifactorial origin. The objective of the present study was to describe for the first time a pathological process characterized by forebrain neuropil vacuolization in pigs showing wasting without conspicuous neurological signs. To characterize the lesions pathologically, affected and non-affected pigs from eight of these farms were investigated. Histologically, the most consistent lesion was neuropil vacuolization of the prosencephalon, mainly located in the thalamic nuclei and in the transition between the white and grey matter of the neocortex (40/56 in sick and 4/30 in healthy pigs). In the most severe cases, the vacuolation also involved the midbrain, cerebellar nuclei and, to a lesser extent, the medulla oblongata. Vacuolization of the forebrain was associated with pigs experiencing marked emaciation and growth retardation. Although the specific cause of the present case remained unknown, the preventive use of multivitamin and mineral complexes in drinking water ameliorated the condition, strongly suggesting a metabolic origin of the observed condition. 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