21af0f7b45e09d7cbdf639782eae559f pmc_10383790.pdf 4ae84df4e1efb03853bcf2068e80f1b223cd4704 pmc_10383790.pdf af57e46d3372ab36a1db887b7d092aaef63a79f84dd1a6a64546ac1409decec4 pmc_10383790.pdf Title: Cetylpyridinium Chloride-Containing Mouthwashes Show Virucidal Activity against Herpes Simplex Virus Type 1 Subject: The oral cavity is particularly susceptible to viral infections that are self-recovering in most cases. However, complications may appear in severe cases and/or immunocompromised subjects. Cetylpyridinium chloride (CPC)-containing mouthwashes are able to decrease the infectivity of the SARS-CoV-2 virus by disrupting the integrity of the viral envelope. Here, we show that CPC, as the active ingredient contained in commercialized, exerts significant antiviral activity against enveloped viruses, such as HSV-1, but not against non-enveloped viruses, such as HPV. CPC-containing mouthwashes have been used as antiseptics for decades, and thus, they can represent a cost-effective measure to limit infection and spread of enveloped viruses infecting the oral cavity, aiding in reducing viral transmission. Keywords: HSV; HPV; CPC; oral infection; enveloped viruses Author: Eva Riveira-Muñoz, Edurne Garcia-Vidal, Manuel Bañó-Polo, Rubén León, Vanessa Blanc, Bonaventura Clotet and Ester Ballana Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 27 08:56:08 2023 CEST ModDate: Tue Jun 27 09:00:48 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1171461 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OHKNFJ+VnURWPalladioL Type 1 Custom yes yes no 10 0 WMZRJI+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 KLKXYS+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 OSZHRL+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 HOWKDX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 68 0 AYXMDM+EURM10 Type 1 Builtin yes yes no 72 0 HDENHN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 86 0 HDENHO+PalatinoLinotype CID TrueType Identity-H yes yes yes 89 0 HDENHP+PalatinoLinotype TrueType WinAnsi yes yes no 95 0 HDENIB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 98 0 HDEOIL+PalatinoLinotype TrueType MacRoman yes yes no 101 0 HDENHN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 118 0 HDENHO+PalatinoLinotype CID TrueType Identity-H yes yes yes 121 0 HDENHP+PalatinoLinotype TrueType WinAnsi yes yes no 127 0 HDENIB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 130 0 HDEOIL+PalatinoLinotype TrueType MacRoman yes yes no 133 0 HDEPMG+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 136 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-06-26 02:46:24 CEST RepresentationInformation: pmc_10383790.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-06-20 02:37:45 CEST Size: 1171461 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 258 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: CPC-Containing Mouthwashes Employed Destination: subsection.2.1 Item: Title: Cell Cultures and Viral Stocks Destination: subsection.2.2 Item: Title: Antiviral Activity against HSV and HPV Destination: subsection.2.3 Item: Title: Statistical Analysis Destination: subsection.2.4 Item: Title: Results and Discussion Destination: section.3 Item: Title: References Destination: section.4 Info: Title: Cetylpyridinium Chloride-Containing Mouthwashes Show Virucidal Activity against Herpes Simplex Virus Type 1 Author: Eva Riveira-Muñoz, Edurne Garcia-Vidal, Manuel Bañó-Polo, Rubén León, Vanessa Blanc, Bonaventura Clotet and Ester Ballana Subject: The oral cavity is particularly susceptible to viral infections that are self-recovering in most cases. However, complications may appear in severe cases and/or immunocompromised subjects. Cetylpyridinium chloride (CPC)-containing mouthwashes are able to decrease the infectivity of the SARS-CoV-2 virus by disrupting the integrity of the viral envelope. Here, we show that CPC, as the active ingredient contained in commercialized, exerts significant antiviral activity against enveloped viruses, such as HSV-1, but not against non-enveloped viruses, such as HPV. CPC-containing mouthwashes have been used as antiseptics for decades, and thus, they can represent a cost-effective measure to limit infection and spread of enveloped viruses infecting the oral cavity, aiding in reducing viral transmission. 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