225d461b686c29cef5803261c609c83c pmc_10421217.pdf fb74a9da670c76a5375033c314c2cd2943975e4f pmc_10421217.pdf 2ad3236c242562251857f8fcd6d9052c0d23a6a1f6924f77cee61e39967f63ee pmc_10421217.pdf Title: Nanoceria as Safe Contrast Agents for X-ray CT Imaging Subject: Cerium oxide nanoparticles (CeO2NPs) have exceptional catalytic properties, rendering them highly effective in removing excessive reactive oxygen species (ROS) from biological environments, which is crucial in safeguarding these environments against radiation-induced damage. Additionally, the Ce atom’s high Z number makes it an ideal candidate for utilisation as an X-ray imaging contrast agent. We herein show how the injection of albumin-stabilised 5 nm CeO2NPs into mice revealed substantial enhancement in X-ray contrast, reaching up to a tenfold increase at significantly lower concentrations than commercial or other proposed contrast agents. Remarkably, these NPs exhibited prolonged residence time within the target organs. Thus, upon injection into the tail vein, they exhibited efficient uptake by the liver and spleen, with 85% of the injected dose (%ID) recovered after 7 days. In the case of intratumoral administration, 99% ID of CeO2NPs remained within the tumour throughout the 7-day observation period, allowing for observation of disease dynamics. Mass spectrometry (ICP-MS) elemental analysis confirmed X-ray CT imaging observations. Keywords: nanoceria; CeO2 nanoparticles; X-ray CT imaging; reactive oxygen species scavengers Author: Ana García, Juan Antonio Cámara, Ana María Boullosa, Muriel F. Gustà, Laura Mondragón, Simó Schwartz, Jr., Eudald Casals, Ibane Abasolo, Neus G. Bastús and Víctor Puntes Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jul 31 06:00:37 2023 CEST ModDate: Mon Jul 31 06:10:52 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2034999 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KORLVE+VnURWPalladioL Type 1 Custom yes yes no 10 0 YRJXTP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 DMUJVX+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 CQJZML+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 CCDETI+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 NPLVPL+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 MJSSOL+CMSY10 Type 1 Builtin yes yes no 68 0 OYHYNK+EURM10 Type 1 Builtin yes yes no 72 0 XIRVNA+EURB10 Type 1 Builtin yes yes no 80 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 88 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 92 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 96 0 BCDIEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 100 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 107 0 BCDMEE+Calibri-Bold TrueType WinAnsi yes yes no 115 0 Arial-BoldMT TrueType WinAnsi no no no 119 0 BCDLEE+Calibri TrueType WinAnsi yes yes no 122 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 141 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 145 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 149 0 BCDIEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 153 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 160 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 177 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 181 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 185 0 BCDIEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 189 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 196 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 213 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 217 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 221 0 BCDIEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 225 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 232 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-10-24 02:24:45 CEST RepresentationInformation: pmc_10421217.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-10-11 02:19:38 CEST Size: 2034999 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 465 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: Materials Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: CeO2NPs Synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Characterisation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Children: Item: Title: UV-Vis Spectroscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Dynamic Light Scattering (DLS) and -Potential Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Transmission Electron Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Ce3+ Concentration by Xylenol Orange Test Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: In Vivo Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: ICP-MS Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: CT Imaging Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Info: Title: Nanoceria as Safe Contrast Agents for X-ray CT Imaging Author: Ana García, Juan Antonio Cámara, Ana María Boullosa, Muriel F. Gustà, Laura Mondragón, Simó Schwartz, Jr., Eudald Casals, Ibane Abasolo, Neus G. Bastús and Víctor Puntes Subject: Cerium oxide nanoparticles (CeO2NPs) have exceptional catalytic properties, rendering them highly effective in removing excessive reactive oxygen species (ROS) from biological environments, which is crucial in safeguarding these environments against radiation-induced damage. Additionally, the Ce atom’s high Z number makes it an ideal candidate for utilisation as an X-ray imaging contrast agent. We herein show how the injection of albumin-stabilised 5 nm CeO2NPs into mice revealed substantial enhancement in X-ray contrast, reaching up to a tenfold increase at significantly lower concentrations than commercial or other proposed contrast agents. Remarkably, these NPs exhibited prolonged residence time within the target organs. Thus, upon injection into the tail vein, they exhibited efficient uptake by the liver and spleen, with 85% of the injected dose (%ID) recovered after 7 days. In the case of intratumoral administration, 99% ID of CeO2NPs remained within the tumour throughout the 7-day observation period, allowing for observation of disease dynamics. Mass spectrometry (ICP-MS) elemental analysis confirmed X-ray CT imaging observations. 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