6af2f818f210f5776a62d63233e99ab9 pmc_10452195.pdf bdd435beb7865f2f10c9e4b64dd7158b30d26d9e pmc_10452195.pdf a3c0ad676088a37aeea2a31ba85336415c895a1ac248f8783a7718596e3ef04a pmc_10452195.pdf Title: Clinical Performance of Lateral Flow Assay for Cryptosporidium spp. Diagnosis Subject: Cryptosporidium spp. is an apicomplexan protozoan parasite associated with gastroenteritis in humans. In 2018, Spain showed 1511 confirmed cases, with a growing trend since 2014. Despite this fact, Cryptosporidium spp. is not usually routinely examined when a parasitological study is ordered, although accurate diagnosis is fundamental to prevent the spread of the illness. The main objectives of the present work is to demonstrate the circulation and to study the epidemiology of cryptosporidiosis in patients who were being tested for the presence of Cryptosporidium spp. parasites in the faeces in the Metropolitan North Area of Barcelona, Maresme, and Vallés Occidental using a two-step algorithm. The stool samples were analysed using the Cryptosporidium/Giardia spp. immunochromatographic test; the positive samples were visualised under a microscope using auramine staining. The proportion of Cryptosporidium spp. cases was around 2% in the studied patients, with a pronounced seasonal incidence peak in late summer–early autumn. In our cohort, weight loss was the main symptom related to confirmed cases. The mean age of confirmed patients was 19 years old, and they were younger than the unconfirmed group. Cryptosporidium spp. is one of the parasites that currently circulate in many areas in Europe. Prevalence must be taken into account for active searching. Keywords: Cryptosporidium spp.; epidemiology; lateral flow; antigen detection Author: Miriam Campos-Ruiz, Clara Flamarich, Anabel Fernández-Navarro, Silvia Roure, Laura Martin, Pablo Pillado, Pere-Joan Cardona and Gema Fernández-Rivas Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Oct 10 03:30:47 2023 CEST ModDate: Wed Oct 11 04:07:37 2023 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 918296 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HCRKXL+VnURWPalladioL Type 1 Custom yes yes no 10 0 GSJKVK+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 QRKJTK+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 LXWGVI+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 QOOPIF+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 EBFBTV+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 OICMGB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 92 0 OICMGC+PalatinoLinotype CID TrueType Identity-H yes yes yes 95 0 OICMGD+PalatinoLinotype TrueType WinAnsi yes yes no 101 0 OICMGF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 104 0 OICNCE+PalatinoLinotype TrueType MacRoman yes yes no 107 0 OICMGB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 125 0 OICMGC+PalatinoLinotype CID TrueType Identity-H yes yes yes 128 0 OICMGD+PalatinoLinotype TrueType WinAnsi yes yes no 134 0 OICMGF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 137 0 OICNCE+PalatinoLinotype TrueType MacRoman yes yes no 140 0 SYVAVM+VnURWPalladioL-Italic Type 1 Custom yes yes no 159 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-03-12 02:12:11 CET RepresentationInformation: pmc_10452195.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-03-12 02:04:28 CET Size: 918296 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 276 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Antigen Detection of Cryptosporidium spp. Oocysts in Faeces Destination: subsection.2.1 Item: Title: Observation of Cryptosporidium spp. Oocyst in Faeces Destination: subsection.2.2 Item: Title: Data Collection Destination: subsection.2.3 Item: Title: Statistical Analysis Destination: subsection.2.4 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Clinical Performance of Lateral Flow Assay for Cryptosporidium spp. Diagnosis Author: Miriam Campos-Ruiz, Clara Flamarich, Anabel Fernández-Navarro, Silvia Roure, Laura Martin, Pablo Pillado, Pere-Joan Cardona and Gema Fernández-Rivas Subject: Cryptosporidium spp. is an apicomplexan protozoan parasite associated with gastroenteritis in humans. In 2018, Spain showed 1511 confirmed cases, with a growing trend since 2014. Despite this fact, Cryptosporidium spp. is not usually routinely examined when a parasitological study is ordered, although accurate diagnosis is fundamental to prevent the spread of the illness. The main objectives of the present work is to demonstrate the circulation and to study the epidemiology of cryptosporidiosis in patients who were being tested for the presence of Cryptosporidium spp. parasites in the faeces in the Metropolitan North Area of Barcelona, Maresme, and Vallés Occidental using a two-step algorithm. The stool samples were analysed using the Cryptosporidium/Giardia spp. immunochromatographic test; the positive samples were visualised under a microscope using auramine staining. The proportion of Cryptosporidium spp. cases was around 2% in the studied patients, with a pronounced seasonal incidence peak in late summer early autumn. In our cohort, weight loss was the main symptom related to confirmed cases. The mean age of confirmed patients was 19 years old, and they were younger than the unconfirmed group. Cryptosporidium spp. is one of the parasites that currently circulate in many areas in Europe. Prevalence must be taken into account for active searching. 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