20ac1bb4cccf3f9de8f2f7db41cea228 pmc_10499179.pdf fd80f069deb50a95fe734bd290083425a474bafb pmc_10499179.pdf bc7989efd6c24038191f8d6369676e17965cf59d773e86373d1a36ffddaca3ae pmc_10499179.pdf Title: Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model Subject: Sanahuja I, Fernandez-Alacid L, Torrecillas S, Ruiz A, Vallejos-Vidal E, Firmino JP, Reyes-Lopez FE, Tort L, Tovar-Ramirez D, Ibarz A and Gisbert E (2023) Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model. Front. Immunol. 14:1247199 Keywords: Debaryomyces hansenii, skin defense, skin mucus defense, skin mucus associated biomarkers, transcriptomics, yeast probiotic Author: Ignasi Sanahuja CreationDate: Fri Aug 25 12:31:30 2023 CEST ModDate: Fri Aug 25 12:31:30 2023 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.389 x 841.663 pts (A4) Page rot: 0 File size: 10277027 bytes Optimized: yes PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OPMNJE+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 1037 0 OPMNLE+AdvTT749c1f5a.I Type 1C WinAnsi yes yes no 1038 0 OPMNLF+AdvTTd3a5f740+fb Type 1C Custom yes yes yes 1039 0 OPMNLG+AdvTT9b12cd41 Type 1C WinAnsi yes yes no 1040 0 OPMNMH+AdvPSA323 Type 1C WinAnsi yes yes no 1041 0 OPMNMI+AdvTT9b12cd41+01 Type 1C MacRoman yes yes no 1042 0 OPMNMJ+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 1053 0 OPMNNJ+AdvTT9b12cd41+fb Type 1C Custom yes yes yes 1054 0 OPMOKF+AdvTT2c8ce45a Type 1C WinAnsi yes yes no 1061 0 OPNCDB+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 445 0 OPNCDC+AdvOT1ef757c0+20 Type 1C WinAnsi yes yes no 453 0 OPNCED+AdvOT1ef757c0+fb Type 1C Custom yes yes yes 454 0 OPMOOF+AdvTT9b12cd41+20 Type 1C WinAnsi yes yes no 459 0 OPMOOG+AdvTTc8b77491.I Type 1C WinAnsi yes yes no 456 0 OPNCFD+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 467 0 OPNCFE+AdvOT3f84ef53 Type 1C WinAnsi yes yes no 468 0 OPNCGF+AdvOT1ef757c0+01 Type 1C MacRoman yes yes no 465 0 OPNCGG+AdvTT58d178b9+03 Type 1C Custom yes yes yes 471 0 OPNCGH+AdvOTd877c31c Type 1C WinAnsi yes yes no 463 0 OPNCHH+AdvOTd877c31c+21 Type 1C WinAnsi yes yes no 478 0 OPNCJI+AdvOT1ef757c0+22 Type 1C Custom yes yes yes 484 0 OPNDEE+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 486 0 OPNFKG+AdvOTb65e897d.B+20 Type 1C WinAnsi yes yes no 488 0 OPOLFI+AdvTTd3a5f740+20 Type 1C WinAnsi yes yes no 491 0 OPOLFJ+AdvOT7d6df7ab.I+fb Type 1C Custom yes yes yes 492 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-03-12 02:11:36 CET RepresentationInformation: pmc_10499179.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-03-12 02:04:19 CET Size: 10277027 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 1125 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model Destination: bmark_-718206375 Children: Item: Title: 1 Introduction Destination: s1 Item: Title: 2 Materials and methods Destination: s2 Children: Item: Title: 2.1 Diet composition Destination: s2_1 Item: Title: 2.2 Fish and experimental model design Destination: s2_2 Item: Title: 2.3 Skin mucus analyses Destination: s2_3 Children: Item: Title: 2.3.1 Skin mucus collection and associated biomarkers Destination: s2_3_1 Item: Title: 2.3.2 Bacterial growth assessment in skin mucus Destination: s2_3_2 Item: Title: 2.4 Skin transcriptional analysis Destination: s2_4 Children: Item: Title: 2.4.1 RNA isolation and quality control Destination: s2_4_1 Item: Title: 2.4.2 Microarray hybridization and analysis Destination: s2_4_2 Item: Title: 2.5 Statistical analysis Destination: s2_5 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 Key performance indicators Destination: s3_1 Item: Title: 3.2 Skin mucus analyses Destination: s3_2 Children: Item: Title: 3.2.1 Skin mucus associated biomarkers Destination: s3_2_1 Item: Title: 3.2.2 Skin mucus antibacterial capacity Destination: s3_2_2 Item: Title: 3.3 Skin transcriptomics Destination: s3_3 Children: Item: Title: 3.3.1 Transcriptomic profile Destination: s3_3_1 Item: Title: 3.3.2 Functional enrichment analysis Destination: s3_3_2 Item: Title: 4 Discussion Destination: s4 Children: Item: Title: 4.1 Modulatory effects of dietary D.’hansenii supplementation Destination: s4_1 Item: Title: 5 Conclusions Destination: s5 Item: Title: Data availability statement Destination: s6 Item: Title: Ethics statement Destination: s7 Item: Title: Author contributions Destination: s8 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model Author: Ignasi Sanahuja Subject: Sanahuja I, Fernandez-Alacid L, Torrecillas S, Ruiz A, Vallejos-Vidal E, Firmino JP, Reyes-Lopez FE, Tort L, Tovar-Ramirez D, Ibarz A and Gisbert E (2023) Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model. Front. Immunol. 14:1247199 Keywords: Debaryomyces hansenii, skin defense, skin mucus defense, skin mucus associated biomarkers, transcriptomics, yeast probiotic CreationDate: Fri Aug 25 12:31:30 CEST 2023 ModDate: Fri Aug 25 12:31:30 CEST 2023 ID: 0x8efa8634c939f575e5100f7b28ea4a34, 0x40cd50750eae304f81fad863ab0ee011 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2919 ImageHeight: 577 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 3807 ImageHeight: 2285 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1864 ImageHeight: 1415 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1862 ImageHeight: 1458 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1847 ImageHeight: 1451 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2304 ImageHeight: 3332 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 3078 ImageHeight: 3092 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1969 ImageHeight: 2787 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2731 ImageHeight: 2076 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2079 ImageHeight: 1345 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2148 ImageHeight: 1265 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 3150 ImageHeight: 1679 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2229 ImageHeight: 1850 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2031 ImageHeight: 942 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 3216 ImageHeight: 1672 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type1: Font: BaseFont: OPNCDC+AdvOT1ef757c0+20 FontSubset: true FirstChar: 137 LastChar: 150 FontDescriptor: FontName: OPNCDC+AdvOT1ef757c0+20 Flags: Nonsymbolic FontBBox: -135, -187, 1010, 833 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNCED+AdvOT1ef757c0+fb FontSubset: true FirstChar: 1 LastChar: 3 FontDescriptor: FontName: OPNCED+AdvOT1ef757c0+fb Flags: Symbolic FontBBox: 0, -10, 781, 708 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPMOOG+AdvTTc8b77491.I FontSubset: true FirstChar: 46 LastChar: 121 FontDescriptor: FontName: OPMOOG+AdvTTc8b77491.I Flags: Nonsymbolic FontBBox: -145, -208, 1020, 895 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMOOF+AdvTT9b12cd41+20 FontSubset: true FirstChar: 146 LastChar: 146 FontDescriptor: FontName: OPMOOF+AdvTT9b12cd41+20 Flags: Nonsymbolic FontBBox: -114, -166, 1010, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNJE+AdvTTd3a5f740 FontSubset: true FirstChar: 37 LastChar: 122 FontDescriptor: FontName: OPMNJE+AdvTTd3a5f740 Flags: Serif, Nonsymbolic FontBBox: -52, -208, 916, 885 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNLE+AdvTT749c1f5a.I FontSubset: true FirstChar: 37 LastChar: 121 FontDescriptor: FontName: OPMNLE+AdvTT749c1f5a.I Flags: Nonsymbolic FontBBox: -145, -208, 989, 885 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNLF+AdvTTd3a5f740+fb FontSubset: true FirstChar: 1 LastChar: 3 FontDescriptor: FontName: OPMNLF+AdvTTd3a5f740+fb Flags: Symbolic FontBBox: 0, 0, 906, 708 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPNCGH+AdvOTd877c31c FontSubset: true FirstChar: 174 LastChar: 174 FontDescriptor: FontName: OPNCGH+AdvOTd877c31c Flags: Nonsymbolic FontBBox: -62, -208, 927, 958 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNLG+AdvTT9b12cd41 FontSubset: true FirstChar: 37 LastChar: 224 FontDescriptor: FontName: OPMNLG+AdvTT9b12cd41 Flags: Serif, Nonsymbolic FontBBox: -52, -208, 906, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNMH+AdvPSA323 FontSubset: true FirstChar: 180 LastChar: 180 FontDescriptor: FontName: OPMNMH+AdvPSA323 Flags: Nonsymbolic FontBBox: -156, -239, 1104, 770 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNCGF+AdvOT1ef757c0+01 FontSubset: true FirstChar: 245 LastChar: 245 FontDescriptor: FontName: OPNCGF+AdvOT1ef757c0+01 Flags: Serif, Nonsymbolic FontBBox: -20, -250, 947, 854 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: OPMNMI+AdvTT9b12cd41+01 FontSubset: true FirstChar: 245 LastChar: 245 FontDescriptor: FontName: OPMNMI+AdvTT9b12cd41+01 Flags: Nonsymbolic FontBBox: -52, -208, 958, 875 FontFile3: true Encoding: MacRomanEncoding Font: BaseFont: OPNCFD+AdvOT7d6df7ab.I FontSubset: true FirstChar: 44 LastChar: 122 FontDescriptor: FontName: OPNCFD+AdvOT7d6df7ab.I Flags: Nonsymbolic FontBBox: -135, -250, 1000, 854 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNCFE+AdvOT3f84ef53 FontSubset: true FirstChar: 98 LastChar: 109 FontDescriptor: FontName: OPNCFE+AdvOT3f84ef53 Flags: Nonsymbolic FontBBox: -177, -291, 1083, 1000 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNCGG+AdvTT58d178b9+03 FontSubset: true FirstChar: 0 LastChar: 0 FontDescriptor: FontName: OPNCGG+AdvTT58d178b9+03 Flags: Symbolic FontBBox: -645, -354, 916, 895 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPMNMJ+AdvTTd168d80a.I FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: OPMNMJ+AdvTTd168d80a.I Flags: Serif, Nonsymbolic FontBBox: -145, -208, 968, 875 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPMNNJ+AdvTT9b12cd41+fb FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: OPMNNJ+AdvTT9b12cd41+fb Flags: Symbolic FontBBox: 0, 0, 854, 697 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPNCHH+AdvOTd877c31c+21 FontSubset: true FirstChar: 153 LastChar: 153 FontDescriptor: FontName: OPNCHH+AdvOTd877c31c+21 Flags: Nonsymbolic FontBBox: -10, -250, 1145, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNCJI+AdvOT1ef757c0+22 FontSubset: true FirstChar: 2 LastChar: 21 FontDescriptor: FontName: OPNCJI+AdvOT1ef757c0+22 Flags: Symbolic FontBBox: -135, -166, 697, 916 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPMOKF+AdvTT2c8ce45a FontSubset: true FirstChar: 37 LastChar: 121 FontDescriptor: FontName: OPMOKF+AdvTT2c8ce45a Flags: Nonsymbolic FontBBox: -52, -208, 947, 895 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNDEE+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 65 LastChar: 67 FontDescriptor: FontName: OPNDEE+HelveticaNeueLTStd-Bd Flags: Nonsymbolic, ForceBold FontBBox: -166, -218, 1078, 975 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPNFKG+AdvOTb65e897d.B+20 FontSubset: true FirstChar: 150 LastChar: 150 FontDescriptor: FontName: OPNFKG+AdvOTb65e897d.B+20 Flags: Nonsymbolic FontBBox: -125, -177, 1052, 822 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPOLFI+AdvTTd3a5f740+20 FontSubset: true FirstChar: 146 LastChar: 146 FontDescriptor: FontName: OPOLFI+AdvTTd3a5f740+20 Flags: Nonsymbolic FontBBox: -114, -166, 1062, 864 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OPOLFJ+AdvOT7d6df7ab.I+fb FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: OPOLFJ+AdvOT7d6df7ab.I+fb Flags: Symbolic FontBBox: -156, -250, 802, 708 FontFile3: true EncodingDictionary: Differences: true ToUnicode: true Font: BaseFont: OPNCDB+AdvOT1ef757c0 FontSubset: true FirstChar: 35 LastChar: 248 FontDescriptor: FontName: OPNCDB+AdvOT1ef757c0 Flags: Serif, Nonsymbolic FontBBox: -20, -250, 947, 854 FontFile3: true Encoding: WinAnsiEncoding XMP: application/pdf Ignasi Sanahuja Laura Fernandez-Alacid Silvia Torrecillas Alberto Ruiz Eva Vallejos-Vidal Joana P. Firmino Felipe E. Reyes-Lopez Lluis Tort Dariel Tovar-Ramirez Antoni Ibarz Enric Gisbert Sanahuja I, Fernandez-Alacid L, Torrecillas S, Ruiz A, Vallejos-Vidal E, Firmino JP, Reyes-Lopez FE, Tort L, Tovar-Ramirez D, Ibarz A and Gisbert E (2023) Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model. Front. Immunol. 14:1247199 Copyright © 2023 Sanahuja, Fernandez-Alacid, Torrecillas, Ruiz, Vallejos-Vidal, Firmino, Reyes-Lopez, Tort, Tovar-Ramirez, Ibarz and Gisbert. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Debaryomyces hansenii skin defense skin mucus defense skin mucus associated biomarkers transcriptomics yeast probiotic Dietary Debaryomyces hansenii promotes skin and skin mucus defensive capacities in a marine fish model Debaryomyces hansenii, skin defense, skin mucus defense, skin mucus associated biomarkers, transcriptomics, yeast probiotic True https://www.frontiersin.org/legal/copyright-statement 2023-08-25T18:31:30+08:00 2023-08-25T18:31:30+08:00 2023-08-25T18:31:30+08:00 uuid:4c995c50-ed37-461e-8ed8-dc70a5f90618 uuid:66629870-70c0-4f48-9c5e-a5c7f0dfc995 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 222, 676, 525, 697 Annotation: Subtype: Link Rect: 222, 651, 517, 672 Annotation: Subtype: Link Rect: 222, 626, 530, 647 Annotation: Subtype: Link Rect: 222, 601, 324, 622 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Annotation: Subtype: Link Rect: 49, 681, 125, 696 Annotation: Subtype: Link Rect: 60, 528, 134, 535 Annotation: Subtype: Link Rect: 49, 398, 158, 405 Annotation: Subtype: Link Rect: 155, 339, 180, 346 Annotation: Subtype: Link Rect: 49, 330, 168, 337 Annotation: Subtype: Link Rect: 450, 802, 506, 809 Annotation: Subtype: Link Rect: 466, 793, 517, 800 Annotation: Subtype: Link Rect: 446, 783, 539, 790 Annotation: Subtype: Link Rect: 49, 797, 233, 813 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 52, 170, 56, 179 Destination: 310 Annotation: Subtype: Link Rect: 110, 132, 114, 141 Destination: 310 Annotation: Subtype: Link Rect: 199, 107, 203, 116 Destination: 310 Annotation: Subtype: Link Rect: 92, 70, 96, 79 Destination: 310 Annotation: Subtype: Link Rect: 101, 70, 105, 79 Destination: 310 Annotation: Subtype: Link Rect: 453, 195, 457, 204 Destination: 310 Annotation: Subtype: Link Rect: 357, 157, 361, 166 Destination: 310 Annotation: Subtype: Link Rect: 367, 157, 371, 166 Destination: 310 Annotation: Subtype: Link Rect: 459, 132, 467, 141 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 263, 744, 271, 753 Destination: 310 Annotation: Subtype: Link Rect: 277, 744, 285, 753 Destination: 310 Annotation: Subtype: Link Rect: 161, 694, 170, 703 Destination: 310 Annotation: Subtype: Link Rect: 277, 681, 285, 690 Destination: 310 Annotation: Subtype: Link Rect: 160, 631, 168, 640 Destination: 310 Annotation: Subtype: Link Rect: 124, 569, 133, 578 Destination: 310 Annotation: Subtype: Link Rect: 138, 569, 146, 578 Destination: 310 Annotation: Subtype: Link Rect: 170, 556, 179, 565 Destination: 310 Annotation: Subtype: Link Rect: 184, 556, 193, 565 Destination: 310 Annotation: Subtype: Link Rect: 197, 556, 206, 565 Destination: 310 Annotation: Subtype: Link Rect: 176, 531, 184, 540 Destination: 310 Annotation: Subtype: Link Rect: 191, 531, 199, 540 Destination: 310 Annotation: Subtype: Link Rect: 184, 456, 192, 465 Destination: 310 Annotation: Subtype: Link Rect: 196, 456, 204, 465 Destination: 310 Annotation: Subtype: Link Rect: 208, 456, 216, 465 Destination: 310 Annotation: Subtype: Link Rect: 220, 456, 228, 465 Destination: 310 Annotation: Subtype: Link Rect: 98, 431, 107, 440 Destination: 310 Annotation: Subtype: Link Rect: 170, 381, 178, 390 Destination: 310 Annotation: Subtype: Link Rect: 277, 369, 285, 378 Destination: 310 Annotation: Subtype: Link Rect: 96, 331, 104, 340 Destination: 310 Annotation: Subtype: Link Rect: 108, 331, 116, 340 Destination: 310 Annotation: Subtype: Link Rect: 171, 319, 180, 328 Destination: 310 Annotation: Subtype: Link Rect: 184, 319, 192, 328 Destination: 310 Annotation: Subtype: Link Rect: 206, 81, 233, 90 Destination: 57 Annotation: Subtype: Link Rect: 408, 731, 435, 740 Destination: 57 Annotation: Subtype: Link Rect: 424, 719, 433, 728 Destination: 310 Annotation: Subtype: Link Rect: 437, 719, 446, 728 Destination: 310 Annotation: Subtype: Link Rect: 451, 719, 460, 728 Destination: 310 Annotation: Subtype: Link Rect: 534, 681, 542, 690 Destination: 310 Annotation: Subtype: Link Rect: 521, 644, 530, 653 Destination: 310 Annotation: Subtype: Link Rect: 535, 644, 543, 653 Destination: 310 Annotation: Subtype: Link Rect: 304, 631, 313, 640 Destination: 310 Annotation: Subtype: Link Rect: 475, 619, 484, 628 Destination: 310 Annotation: Subtype: Link Rect: 352, 131, 360, 140 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 328, 216, 337, 225 Destination: 310 Annotation: Subtype: Link Rect: 501, 153, 509, 162 Destination: 310 Annotation: Subtype: Link Rect: 362, 116, 370, 125 Destination: 358 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 170, 756, 178, 765 Destination: 310 Annotation: Subtype: Link Rect: 118, 644, 126, 653 Destination: 310 Annotation: Subtype: Link Rect: 222, 467, 231, 476 Destination: 358 Annotation: Subtype: Link Rect: 222, 118, 230, 127 Destination: 358 Annotation: Subtype: Link Rect: 422, 531, 493, 540 Annotation: Subtype: Link Rect: 499, 469, 508, 478 Destination: 358 Annotation: Subtype: Link Rect: 512, 469, 520, 478 Destination: 358 Annotation: Subtype: Link Rect: 308, 406, 385, 415 Annotation: Subtype: Link Rect: 397, 406, 406, 415 Destination: 358 Annotation: Subtype: Link Rect: 475, 406, 542, 415 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 6 Annotations: Annotation: Subtype: Link Rect: 230, 684, 258, 693 Destination: 82 Annotation: Subtype: Link Rect: 261, 584, 288, 593 Destination: 82 Annotation: Subtype: Link Rect: 432, 707, 458, 716 Destination: 82 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 244, 719, 274, 728 Destination: 91 Annotation: Subtype: Link Rect: 355, 756, 387, 765 Destination: 91 Annotation: Subtype: Link Rect: 482, 756, 513, 765 Destination: 110 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 49, 437, 85, 446 Destination: 91 Annotation: Subtype: Link Rect: 93, 387, 130, 396 Destination: 91 Annotation: Subtype: Link Rect: 189, 362, 226, 371 Destination: 91 Annotation: Subtype: Link Rect: 99, 312, 135, 321 Destination: 91 Annotation: Subtype: Link Rect: 207, 300, 237, 309 Destination: 110 Annotation: Subtype: Link Rect: 307, 437, 337, 446 Destination: 132 Annotation: Subtype: Link Rect: 355, 437, 438, 446 Destination: 310 Annotation: Subtype: Link Rect: 304, 400, 340, 409 Destination: 132 Annotation: Subtype: Link Rect: 343, 287, 380, 296 Destination: 132 Annotation: Subtype: Link Rect: 381, 262, 416, 271 Destination: 132 Annotation: Subtype: Link Rect: 511, 225, 541, 234 Destination: 155 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 9 Annotations: Annotation: Subtype: Link Rect: 52, 145, 81, 154 Destination: 171 Annotation: Subtype: Link Rect: 99, 145, 179, 154 Destination: 310 Annotation: Subtype: Link Rect: 132, 95, 164, 104 Destination: 187 Annotation: Subtype: Link Rect: 168, 95, 176, 104 Destination: 230 Annotation: Subtype: Link Rect: 193, 95, 273, 104 Destination: 310 Annotation: Subtype: Link Rect: 239, 83, 269, 92 Destination: 187 Annotation: Subtype: Link Rect: 441, 158, 469, 167 Destination: 187 Annotation: Subtype: Link Rect: 421, 120, 449, 129 Destination: 197 Annotation: Subtype: Link Rect: 476, 83, 509, 92 Destination: 197 Annotation: Subtype: Link Rect: 509, 70, 541, 79 Destination: 213 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 10 Annotations: Annotation: Subtype: Link Rect: 52, 299, 85, 308 Destination: 213 Annotation: Subtype: Link Rect: 231, 287, 264, 296 Destination: 230 Annotation: Subtype: Link Rect: 115, 237, 146, 246 Destination: 171 Annotation: Subtype: Link Rect: 364, 262, 372, 271 Destination: 358 Annotation: Subtype: Link Rect: 393, 200, 401, 209 Destination: 358 Annotation: Subtype: Link Rect: 408, 200, 416, 209 Destination: 358 Annotation: Subtype: Link Rect: 519, 162, 527, 171 Destination: 358 Annotation: Subtype: Link Rect: 532, 162, 541, 171 Destination: 358 Annotation: Subtype: Link Rect: 234, 380, 306, 387 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 11 Annotations: Annotation: Subtype: Link Rect: 214, 111, 223, 120 Destination: 358 Annotation: Subtype: Link Rect: 452, 236, 460, 245 Destination: 358 Annotation: Subtype: Link Rect: 466, 236, 474, 245 Destination: 358 Annotation: Subtype: Link Rect: 411, 211, 420, 220 Destination: 358 Annotation: Subtype: Link Rect: 452, 199, 460, 208 Destination: 310 Annotation: Subtype: Link Rect: 355, 136, 364, 145 Destination: 310 Annotation: Subtype: Link Rect: 370, 136, 378, 145 Destination: 310 Annotation: Subtype: Link Rect: 384, 136, 393, 145 Destination: 358 Annotation: Subtype: Link Rect: 108, 283, 181, 290 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 12 Annotations: Annotation: Subtype: Link Rect: 174, 510, 247, 517 Destination: 310 Annotation: Subtype: Link Rect: 231, 81, 304, 88 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 13 Annotations: Annotation: Subtype: Link Rect: 143, 428, 147, 437 Destination: 310 Annotation: Subtype: Link Rect: 153, 428, 162, 437 Destination: 358 Annotation: Subtype: Link Rect: 307, 466, 316, 475 Destination: 358 Annotation: Subtype: Link Rect: 333, 403, 342, 412 Destination: 358 Annotation: Subtype: Link Rect: 342, 353, 346, 362 Destination: 310 Annotation: Subtype: Link Rect: 352, 353, 360, 362 Destination: 358 Annotation: Subtype: Link Rect: 249, 509, 322, 516 Destination: 310 Annotation: Subtype: Link Rect: 231, 81, 304, 88 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 14 Annotations: Annotation: Subtype: Link Rect: 162, 337, 171, 346 Destination: 310 Annotation: Subtype: Link Rect: 175, 337, 184, 346 Destination: 310 Annotation: Subtype: Link Rect: 189, 337, 197, 346 Destination: 358 Annotation: Subtype: Link Rect: 251, 312, 259, 321 Destination: 310 Annotation: Subtype: Link Rect: 265, 312, 274, 321 Destination: 310 Annotation: Subtype: Link Rect: 307, 312, 316, 321 Destination: 310 Annotation: Subtype: Link Rect: 357, 299, 365, 308 Destination: 310 Annotation: Subtype: Link Rect: 231, 434, 304, 441 Destination: 310 Annotation: Subtype: Link Rect: 318, 81, 391, 88 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 15 Annotations: Annotation: Subtype: Link Rect: 100, 469, 109, 478 Destination: 358 Annotation: Subtype: Link Rect: 139, 394, 148, 403 Destination: 358 Annotation: Subtype: Link Rect: 153, 394, 162, 403 Destination: 358 Annotation: Subtype: Link Rect: 253, 369, 262, 378 Destination: 358 Annotation: Subtype: Link Rect: 52, 319, 61, 328 Destination: 310 Annotation: Subtype: Link Rect: 212, 269, 221, 278 Destination: 358 Annotation: Subtype: Link Rect: 105, 194, 114, 203 Destination: 358 Annotation: Subtype: Link Rect: 121, 194, 130, 203 Destination: 358 Annotation: Subtype: Link Rect: 97, 69, 106, 78 Destination: 358 Annotation: Subtype: Link Rect: 449, 481, 457, 490 Destination: 358 Annotation: Subtype: Link Rect: 307, 456, 316, 465 Destination: 310 Annotation: Subtype: Link Rect: 436, 369, 444, 378 Destination: 358 Annotation: Subtype: Link Rect: 449, 369, 457, 378 Destination: 358 Annotation: Subtype: Link Rect: 378, 319, 387, 328 Destination: 358 Annotation: Subtype: Link Rect: 359, 269, 367, 278 Destination: 358 Annotation: Subtype: Link Rect: 372, 169, 381, 178 Destination: 358 Annotation: Subtype: Link Rect: 387, 169, 395, 178 Destination: 358 Annotation: Subtype: Link Rect: 427, 144, 436, 153 Destination: 358 Annotation: Subtype: Link Rect: 231, 531, 304, 538 Destination: 310 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 16 Annotations: Annotation: Subtype: Link Rect: 89, 694, 98, 703 Destination: 358 Annotation: Subtype: Link Rect: 96, 644, 105, 653 Destination: 358 Annotation: Subtype: Link Rect: 76, 544, 84, 553 Destination: 358 Annotation: Subtype: Link Rect: 112, 444, 120, 453 Destination: 358 Annotation: Subtype: Link Rect: 260, 369, 268, 378 Destination: 310 Annotation: Subtype: Link Rect: 83, 319, 87, 328 Destination: 310 Annotation: Subtype: Link Rect: 92, 319, 100, 328 Destination: 358 Annotation: Subtype: Link Rect: 143, 281, 151, 290 Destination: 358 Annotation: Subtype: Link Rect: 241, 256, 249, 265 Destination: 310 Annotation: Subtype: Link Rect: 253, 256, 261, 265 Destination: 310 Annotation: Subtype: Link Rect: 212, 194, 220, 203 Destination: 358 Annotation: Subtype: Link Rect: 199, 156, 208, 165 Destination: 358 Annotation: Subtype: Link Rect: 215, 156, 224, 165 Destination: 358 Annotation: Subtype: Link Rect: 179, 131, 189, 140 Destination: 358 Annotation: Subtype: Link Rect: 203, 106, 211, 115 Destination: 358 Annotation: Subtype: Link Rect: 218, 106, 227, 115 Destination: 358 Annotation: Subtype: Link Rect: 372, 756, 380, 765 Destination: 358 Annotation: Subtype: Link Rect: 466, 631, 474, 640 Destination: 310 Annotation: Subtype: Link Rect: 402, 594, 411, 603 Destination: 358 Annotation: Subtype: Link Rect: 373, 494, 381, 503 Destination: 420 Annotation: Subtype: Link Rect: 520, 419, 525, 428 Destination: 310 Annotation: Subtype: Link Rect: 531, 419, 540, 428 Destination: 420 Annotation: Subtype: Link Rect: 408, 406, 416, 415 Destination: 310 Annotation: Subtype: Link Rect: 419, 319, 427, 328 Destination: 420 Annotation: Subtype: Link Rect: 432, 319, 441, 328 Destination: 420 Annotation: Subtype: Link Rect: 436, 269, 445, 278 Destination: 358 Annotation: Subtype: Link Rect: 368, 194, 377, 203 Destination: 420 Annotation: Subtype: Link Rect: 515, 156, 523, 165 Destination: 420 Annotation: Subtype: Link Rect: 450, 106, 458, 115 Destination: 420 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 17 Annotations: Annotation: Subtype: Link Rect: 199, 756, 207, 765 Destination: 358 Annotation: Subtype: Link Rect: 212, 756, 220, 765 Destination: 420 Annotation: Subtype: Link Rect: 225, 756, 233, 765 Destination: 420 Annotation: Subtype: Link Rect: 280, 694, 288, 703 Destination: 420 Annotation: Subtype: Link Rect: 49, 681, 58, 690 Destination: 420 Annotation: Subtype: Link Rect: 130, 681, 139, 690 Destination: 420 Annotation: Subtype: Link Rect: 112, 619, 120, 628 Destination: 310 Annotation: Subtype: Link Rect: 126, 619, 134, 628 Destination: 310 Annotation: Subtype: Link Rect: 98, 569, 107, 578 Destination: 420 Annotation: Subtype: Link Rect: 113, 569, 122, 578 Destination: 420 Annotation: Subtype: Link Rect: 277, 531, 285, 540 Destination: 310 Annotation: Subtype: Link Rect: 177, 144, 288, 153 Annotation: Subtype: Link Rect: 92, 131, 201, 140 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 18 Annotations: Annotation: Subtype: Link Rect: 304, 640, 545, 649 Annotation: Subtype: Link Rect: 304, 628, 401, 637 Annotation: Subtype: Link Rect: 266, 545, 290, 552 Annotation: Subtype: Link Rect: 49, 537, 120, 544 Annotation: Subtype: Link Rect: 171, 518, 240, 525 Annotation: Subtype: Link Rect: 99, 491, 167, 498 Annotation: Subtype: Link Rect: 175, 464, 254, 471 Annotation: Subtype: Link Rect: 148, 445, 208, 452 Annotation: Subtype: Link Rect: 95, 407, 190, 414 Annotation: Subtype: Link Rect: 175, 380, 270, 387 Annotation: Subtype: Link Rect: 229, 361, 281, 368 Annotation: Subtype: Link Rect: 217, 334, 290, 341 Annotation: Subtype: Link Rect: 95, 307, 148, 314 Annotation: Subtype: Link Rect: 239, 288, 287, 295 Annotation: Subtype: Link Rect: 62, 253, 142, 260 Annotation: Subtype: Link Rect: 182, 226, 279, 233 Annotation: Subtype: Link Rect: 62, 199, 157, 206 Annotation: Subtype: Link Rect: 266, 172, 290, 179 Annotation: Subtype: Link Rect: 49, 164, 122, 171 Annotation: Subtype: Link Rect: 164, 137, 244, 144 Annotation: Subtype: Link Rect: 162, 108, 250, 115 Annotation: Subtype: Link Rect: 102, 71, 170, 78 Annotation: Subtype: Link Rect: 522, 529, 545, 536 Annotation: Subtype: Link Rect: 304, 521, 356, 528 Annotation: Subtype: Link Rect: 432, 486, 513, 493 Annotation: Subtype: Link Rect: 373, 451, 459, 458 Annotation: Subtype: Link Rect: 521, 432, 545, 439 Annotation: Subtype: Link Rect: 304, 424, 340, 431 Annotation: Subtype: Link Rect: 436, 397, 512, 404 Annotation: Subtype: Link Rect: 357, 362, 429, 369 Annotation: Subtype: Link Rect: 409, 335, 481, 342 Annotation: Subtype: Link Rect: 521, 300, 545, 307 Annotation: Subtype: Link Rect: 304, 292, 360, 299 Annotation: Subtype: Link Rect: 317, 265, 385, 272 Annotation: Subtype: Link Rect: 381, 238, 453, 245 Annotation: Subtype: Link Rect: 369, 195, 449, 202 Annotation: Subtype: Link Rect: 317, 160, 368, 167 Annotation: Subtype: Link Rect: 521, 133, 545, 140 Annotation: Subtype: Link Rect: 304, 125, 340, 132 Annotation: Subtype: Link Rect: 388, 90, 450, 97 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 19 Annotations: Annotation: Subtype: Link Rect: 101, 750, 188, 757 Annotation: Subtype: Link Rect: 129, 723, 195, 730 Annotation: Subtype: Link Rect: 206, 688, 274, 695 Annotation: Subtype: Link Rect: 111, 653, 192, 660 Annotation: Subtype: Link Rect: 126, 618, 184, 625 Annotation: Subtype: Link Rect: 139, 591, 180, 598 Annotation: Subtype: Link Rect: 142, 564, 194, 571 Annotation: Subtype: Link Rect: 150, 537, 231, 544 Annotation: Subtype: Link Rect: 145, 502, 214, 509 Annotation: Subtype: Link Rect: 121, 474, 235, 481 Annotation: Subtype: Link Rect: 109, 447, 194, 454 Annotation: Subtype: Link Rect: 146, 428, 209, 435 Annotation: Subtype: Link Rect: 62, 385, 130, 392 Annotation: Subtype: Link Rect: 168, 350, 263, 357 Annotation: Subtype: Link Rect: 141, 307, 203, 314 Annotation: Subtype: Link Rect: 62, 280, 143, 287 Annotation: Subtype: Link Rect: 202, 261, 272, 268 Annotation: Subtype: Link Rect: 241, 242, 290, 249 Annotation: Subtype: Link Rect: 62, 207, 141, 214 Annotation: Subtype: Link Rect: 266, 188, 290, 195 Annotation: Subtype: Link Rect: 49, 180, 88, 187 Annotation: Subtype: Link Rect: 72, 137, 140, 144 Annotation: Subtype: Link Rect: 62, 110, 129, 117 Annotation: Subtype: Link Rect: 105, 81, 172, 88 Annotation: Subtype: Link Rect: 522, 742, 545, 749 Annotation: Subtype: Link Rect: 304, 734, 363, 741 Annotation: Subtype: Link Rect: 522, 715, 545, 722 Annotation: Subtype: Link Rect: 304, 707, 359, 714 Annotation: Subtype: Link Rect: 521, 680, 545, 687 Annotation: Subtype: Link Rect: 304, 672, 379, 679 Annotation: Subtype: Link Rect: 317, 645, 387, 652 Annotation: Subtype: Link Rect: 428, 618, 504, 625 Annotation: Subtype: Link Rect: 378, 591, 434, 598 Annotation: Subtype: Link Rect: 339, 563, 415, 570 Annotation: Subtype: Link Rect: 347, 535, 431, 542 Annotation: Subtype: Link Rect: 522, 515, 545, 522 Annotation: Subtype: Link Rect: 304, 507, 376, 514 Annotation: Subtype: Link Rect: 482, 487, 528, 494 Annotation: Subtype: Link Rect: 384, 451, 456, 458 Annotation: Subtype: Link Rect: 475, 424, 545, 431 Annotation: Subtype: Link Rect: 433, 397, 512, 404 Annotation: Subtype: Link Rect: 438, 378, 514, 385 Annotation: Subtype: Link Rect: 336, 343, 411, 350 Annotation: Subtype: Link Rect: 445, 316, 524, 323 Annotation: Subtype: Link Rect: 405, 289, 474, 296 Annotation: Subtype: Link Rect: 522, 262, 545, 269 Annotation: Subtype: Link Rect: 304, 254, 365, 261 Annotation: Subtype: Link Rect: 471, 235, 545, 242 Annotation: Subtype: Link Rect: 522, 216, 545, 223 Annotation: Subtype: Link Rect: 304, 208, 332, 215 Annotation: Subtype: Link Rect: 361, 181, 453, 188 Annotation: Subtype: Link Rect: 449, 154, 501, 161 Annotation: Subtype: Link Rect: 381, 135, 476, 142 Annotation: Subtype: Link Rect: 522, 108, 545, 115 Annotation: Subtype: Link Rect: 304, 100, 365, 107 Annotation: Subtype: Link Rect: 408, 81, 458, 88 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Page: Label: 20 Annotations: Annotation: Subtype: Link Rect: 185, 742, 239, 749 Annotation: Subtype: Link Rect: 62, 707, 130, 714 Annotation: Subtype: Link Rect: 62, 680, 101, 687 Annotation: Subtype: Link Rect: 264, 661, 290, 668 Annotation: Subtype: Link Rect: 49, 653, 98, 660 Annotation: Subtype: Link Rect: 242, 634, 290, 641 Annotation: Subtype: Link Rect: 62, 599, 139, 606 Annotation: Subtype: Link Rect: 62, 572, 122, 579 Annotation: Subtype: Link Rect: 521, 750, 545, 757 Annotation: Subtype: Link Rect: 304, 742, 351, 749 Annotation: Subtype: Link Rect: 384, 707, 465, 714 Annotation: Subtype: Link Rect: 522, 688, 545, 695 Annotation: Subtype: Link Rect: 304, 680, 375, 687 Annotation: Subtype: Link Rect: 317, 645, 414, 652 Annotation: Subtype: Link Rect: 409, 618, 472, 625 Annotation: Subtype: Link Rect: 522, 591, 545, 597 Annotation: Subtype: Link Rect: 304, 583, 356, 590 Annotation: Subtype: Link Rect: 418, 554, 491, 561 Annotation: Subtype: Link Rect: 450, 799, 545, 806 Annotation: Subtype: Link Rect: 49, 33, 129, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Checksum: c9299bb8 Type: CRC32 Checksum: 20ac1bb4cccf3f9de8f2f7db41cea228 Type: MD5 Checksum: fd80f069deb50a95fe734bd290083425a474bafb Type: SHA-1 Checksum: bc7989efd6c24038191f8d6369676e17965cf59d773e86373d1a36ffddaca3ae Type: SHA-256