5f754561e5672c48f22fbb99c5a32da8 pmc_10528513.pdf b329245dee35fc26501651985bd4be7a9160c0f8 pmc_10528513.pdf 867dfcfff0d026bdd6598f5416630cf93cecf4d465ee78c214ae45b8d8357781 pmc_10528513.pdf Title: Development of Fortified Breads Enriched with Plant-Based Bioactive Peptides Derived from the Chia (Salvia hispanica L.) Expeller Subject: By-products from the industrialization of oilseeds, particularly chia, can be sustainably used for the development of new functional products. In this work, wheat breads supplemented with up to 10 mg of chia expeller hydrolysate/g of flour were prepared, obtaining fortified breads with acceptability for consumption, according to a preliminary consumer research study based on an affective test employing a five-point hedonic scale of global acceptance. In this context, protein hydrolysates of the chia expeller were produced using Alcalase, reaching a degree of hydrolysis of 54.3 1.6% with an antioxidant activity of 55.8 0.4% after 6 h incubation at 25 C in the presence of the enzyme. These peptides showed appropriate techno-functional properties and chemical compositions suitable for the further development of bakery products. Taken together, our approach and the development of a fortified bread with plant-based bioactive peptides provide a novel and eco-friendly alternative for the recovery of nutrients from agro-industrial waste. More importantly, these enriched breads could exert beneficial effects on human health by exploiting the antioxidant properties of functional peptides derived from the chia expeller. Keywords: antioxidant; bioactive peptides; bread; chia expeller; chia by-product; enzymatic hydrolysates; functional ingredient; plant-derived peptides; Salvia hispanica L. Author: Brenda Ozón, Juliana Cotabarren, Florencia R. Geier, M. Paula Kise, Javier García-Pardo, Mónica G. Parisi and W. David Obregón Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Sat Sep 9 09:45:22 2023 CEST ModDate: Sat Sep 9 09:48:39 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1908571 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MFWPRN+VnURWPalladioL Type 1 Custom yes yes no 10 0 EMVOWP+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 RBCFEN+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 TAXQFZ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 OPVWFH+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 QJAFSH+CMSY10 Type 1 Builtin yes yes no 32 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 36 0 YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 40 0 XBNVAE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 58 0 GIGFZE+CMR10 Type 1 Builtin 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Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Materials and Reagents Destination: subsection.2.1 Item: Title: Obtention of the Chia Expeller Protein Hydrolysate Destination: subsection.2.2 Item: Title: Characterization of Hydrolysates Destination: subsection.2.3 Children: Item: Title: Determination of the Degree of Hydrolysis Destination: subsubsection.2.3.1 Item: Title: Evaluation of Antioxidant Activity Destination: subsubsection.2.3.2 Item: Title: Chemical and Techno-Functional Properties of the Chia Expeller Hydrolysates Destination: subsubsection.2.3.3 Item: Title: Ingredients and Breadmaking Procedure Destination: subsection.2.4 Item: Title: Technological and Physicochemical Parameters Destination: subsection.2.5 Children: Item: Title: Specific Volume Determination Destination: subsubsection.2.5.1 Item: Title: Colour Analysis Destination: subsubsection.2.5.2 Item: Title: Texture Profile Analysis Destination: subsubsection.2.5.3 Item: Title: Sensory Evaluation Destination: subsubsection.2.5.4 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Enzymatic Hydrolysis of Chia Expeller Using Alcalase Destination: subsection.3.1 Item: Title: Physicochemical and Techno-Functional Properties of the Chia Expeller Hydrolysates Destination: subsection.3.2 Item: Title: Technological Parameters of the Fortified Breads Enriched with Functional Peptides Derived from the Chia Expeller Destination: subsection.3.3 Children: Item: Title: External Analysis: Crust Colour Destination: subsubsection.3.3.1 Item: Title: Alveolate and Specific Volume Analysis Destination: subsubsection.3.3.2 Item: Title: Texture Profile Analysis Destination: subsubsection.3.3.3 Item: Title: Crumb Colour Analysis Destination: subsubsection.3.3.4 Item: Title: Antioxidant Activity of Baked Breads Fortified with Chia Expeller Hydrolysates Destination: subsection.3.4 Item: Title: Sensory Test: Consumer Acceptability of Selected Bread Fortified with Chia Expeller Hydrolysate (B10) Destination: subsection.3.5 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.A. Info: Title: Development of Fortified Breads Enriched with Plant-Based Bioactive Peptides Derived from the Chia (Salvia hispanica L.) Expeller Author: Brenda Ozón, Juliana Cotabarren, Florencia R. Geier, M. Paula Kise, Javier García-Pardo, Mónica G. Parisi and W. David Obregón Subject: By-products from the industrialization of oilseeds, particularly chia, can be sustainably used for the development of new functional products. In this work, wheat breads supplemented with up to 10 mg of chia expeller hydrolysate/g of flour were prepared, obtaining fortified breads with acceptability for consumption, according to a preliminary consumer research study based on an affective test employing a five-point hedonic scale of global acceptance. In this context, protein hydrolysates of the chia expeller were produced using Alcalase, reaching a degree of hydrolysis of 54.3 1.6% with an antioxidant activity of 55.8 0.4% after 6 h incubation at 25 C in the presence of the enzyme. These peptides showed appropriate techno-functional properties and chemical compositions suitable for the further development of bakery products. Taken together, our approach and the development of a fortified bread with plant-based bioactive peptides provide a novel and eco-friendly alternative for the recovery of nutrients from agro-industrial waste. More importantly, these enriched breads could exert beneficial effects on human health by exploiting the antioxidant properties of functional peptides derived from the chia expeller. Keywords: antioxidant; bioactive peptides; bread; chia expeller; chia by-product; enzymatic hydrolysates; functional ingredient; plant-derived peptides; Salvia hispanica L. 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