d6d8708e0a615eb658310531ac18ce3e pmc_10593445.pdf b8ad9791d9882b650b1c502e472e67799ec33e7e pmc_10593445.pdf ca17d8a73ccc1995e558f12d0e809792188be69054119c7b48a422a36340821c pmc_10593445.pdf Title: Novel frameshift variants expand the map of the genetic defects in IRF2BP2 Subject: García-Aznar JM, Maneiro Pampín E, García Ramos M, Acuña Pérez MJ, Paz Gandiaga N, Minguell Domingo L, Calavia O, Soler-Palacin P, Colobran R, Novoa Bolívar EM and Ocejo Vinyals JG (2023) Novel frameshift variants expand the map of the genetic defects in IRF2BP2. Front. Immunol. 14:1279171 Keywords: IRF2BP2, CVID, colitis, primary immunodeficiency, loss-of-function mutations Author: José María García-Aznar CreationDate: Mon Oct 9 08:16:18 2023 CEST ModDate: Mon Oct 9 08:16:18 2023 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.389 x 841.663 pts (A4) Page rot: 0 File size: 3919586 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IKJCJK+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 761 0 IKJCKL+AdvPSA18C Type 1C WinAnsi yes yes no 762 0 IKJCKM+AdvTTd3a5f740+01 Type 1C MacRoman yes yes no 763 0 IKJCKN+AdvTT9b12cd41 Type 1C WinAnsi yes yes no 764 0 IKJCKO+AdvPSA323 Type 1C WinAnsi yes yes no 765 0 IKJCKP+AdvTT9b12cd41+fb Type 1C Custom yes yes yes 766 0 IKJCLA+AdvTT9b12cd41+20 Type 1C WinAnsi yes yes no 767 0 IKJCLB+AdvTT2c8ce45a Type 1C WinAnsi yes yes no 780 0 IKJCLC+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 783 0 IKJFLB+AdvTT9b12cd41+01 Type 1C MacRoman yes yes no 784 0 IKJGBA+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 171 0 IKJGBB+AdvOT1ef757c0+fb Type 1C Custom yes yes yes 177 0 IKJGBC+AdvOT1ef757c0+20 Type 1C WinAnsi yes yes no 178 0 IKJGAP+AdvTT2c8ce45a+fb Type 1C Custom yes yes yes 179 0 IKJGDD+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 186 0 IKJGED+AdvOT3f84ef53 Type 1C WinAnsi yes yes no 187 0 IKJGEE+AdvOTd877c31c+21 Type 1C WinAnsi yes yes no 188 0 IKJNLH+AdvTTd168d80a.I+20 Type 1C WinAnsi yes yes no 189 0 IKJNLI+AdvTTd168d80a.I+fb Type 1C Custom yes yes yes 190 0 IKKBPA+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 191 0 IKKJAF+AdvTTd3a5f740+fb Type 1C Custom yes yes yes 192 0 IKKJAG+AdvOTb65e897d.B Type 1C WinAnsi yes yes no 195 0 IKKJFF+AdvTT58d178b9+03 Type 1C Custom yes yes yes 202 0 IKKKEB+AdvTTd3a5f740+20 Type 1C WinAnsi yes yes no 205 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-04-12 02:08:40 CEST RepresentationInformation: pmc_10593445.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-04-11 05:19:33 CEST Size: 3919586 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 851 FreeObjects: 3 IncrementalUpdates: 3 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Novel frameshift variants expand the map of the genetic defects in IRF2BP2 Destination: bmark_213701477 Children: Item: Title: 1 Introduction Destination: s1 Item: Title: 2 Materials and methods Destination: s2 Children: Item: Title: 2.1 High throughput sequencing Destination: s2_1 Item: Title: 2.2 Sanger sequencing DNA Destination: s2_2 Item: Title: 2.3 CGX-HD array Destination: s2_3 Item: Title: 2.4 RNA isolation, RT-PCR and cDNA synthesis Destination: s2_4 Item: Title: 2.5 RT-PCR assay of IRF2BP2 endogenous expression Destination: s2_5 Item: Title: 2.6 Sanger sequencing of RT-PCR products Destination: s2_6 Item: Title: 2.7 Patient consent for publication Destination: s2_7 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 Patient description Destination: s3_1 Item: Title: 3.2 Loss-of-function variants in IRF2BP2 Destination: s3_2 Item: Title: 3.3 RT-PCR analysis of endogenous IRFBP2 expression Destination: s3_3 Item: Title: 3.4 Literature review of variants in IRF2BP2 Destination: s3_4 Item: Title: 4 Discussion Destination: s4 Item: Title: Data availability statement Destination: s5 Item: Title: Ethics statement Destination: s6 Item: Title: Author contributions Destination: s7 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Novel frameshift variants expand the map of the genetic defects in IRF2BP2 Author: José María García-Aznar Subject: García-Aznar JM, Maneiro Pampín E, García Ramos M, Acuña Pérez MJ, Paz Gandiaga N, Minguell Domingo L, Calavia O, Soler-Palacin P, Colobran R, Novoa Bolívar EM and Ocejo Vinyals JG (2023) Novel frameshift variants expand the map of the genetic defects in IRF2BP2. Front. 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Novoa Bolívar Javier Gonzalo Ocejo Vinyals García-Aznar JM, Maneiro Pampín E, García Ramos M, Acuña Pérez MJ, Paz Gandiaga N, Minguell Domingo L, Calavia O, Soler-Palacin P, Colobran R, Novoa Bolívar EM and Ocejo Vinyals JG (2023) Novel frameshift variants expand the map of the genetic defects in IRF2BP2. Front. Immunol. 14:1279171 Copyright © 2023 García-Aznar, Maneiro Pampín, García Ramos, Acuña Pérez, Paz Gandiaga, Minguell Domingo, Calavia, Soler-Palacin, Colobran, Novoa Bolívar and Ocejo Vinyals. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 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