71433028068b03024710e8712a250852 pmc_10606246.pdf 8845426a44537ff24a7185a510081f0d4dc53e77 pmc_10606246.pdf 98c54fe3a1acd941cb6c061b7c7105b51a67503c3594e2ced0a62ac6759ba89b pmc_10606246.pdf Title: Drastic Microbial Count Reduction in Soy Milk Using Continuous Short-Wave Ultraviolet Treatments in a Tubular Annular Thin Film UV-C Reactor Subject: Vegetative cells of Listeria monocytogenes and Escherichia coli and spores of Bacillus subtilis and Aspergillus niger were inoculated in soy milk at an initial concentration of 5 log CFU/mL. Inoculated and control (non-inoculated) soy milk samples were submitted to three types of treatments using a tubular annular thin film short-wave ultraviolet (UV-C) reactor with 1 mm of layer thickness. Treatments applied depended on the flow rate and the number of entries to the reactor, with UV-C doses ranging from 20 to 160 J/mL. The number of entries into the reactor tube (NET) was established as the most determining parameter for the efficiency of the UV-C treatments. Conidiospores of A. niger were reported as the most resistant, followed by B. subtilis spores, while vegetative cells were the most sensible to UV-C, with Listeria monocytogenes being more sensible than Escherichia coli. Treatments of just 80 J/mL were needed to achieve a 5 log CFU/mL reduction of L. monocytogenes while 160 J/mL was necessary to achieve a similar reduction for A. niger spores. Keywords: Listeria monocytogenes; Escherichia coli; Bacillus subtilis; Aspergillus niger; soy milk; short-wave ultraviolet (UV-C) Author: María Martínez-García, Jezer N. Sauceda-Gálvez, Idoia Codina-Torrella, María Manuela Hernández-Herrero, Ramón Gervilla and Artur X. Roig-Sagués Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Oct 18 10:57:35 2023 CEST ModDate: Wed Oct 18 11:00:52 2023 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2924500 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OHKNFJ+VnURWPalladioL Type 1 Custom yes yes no 10 0 HTUDLU+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 EGBKUD+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 KRGXCI+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 QQOOTE+CMSY10 Type 1 Builtin yes yes no 28 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 XBNVAE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 YRXHGP+URWPalladioL-BoldItal Type 1 Custom yes yes no 75 0 MFKJDE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 86 0 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yes yes no 267 0 MFKJDI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 270 0 MFKKDD+PalatinoLinotype TrueType MacRoman yes yes no 273 0 MFKJDE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 293 0 MFKJDG+PalatinoLinotype TrueType WinAnsi yes yes no 296 0 MFKJDI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 299 0 MFKJDE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 314 0 MFKJDG+PalatinoLinotype TrueType WinAnsi yes yes no 317 0 MFKJDI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 320 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-11-10 02:32:38 CET RepresentationInformation: pmc_10606246.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-11-08 02:28:09 CET Size: 2924500 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 504 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Children: Item: Title: Soy Milk Physicochemical Characteristics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: UV-C Equipment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: UV-C Treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Dosimetry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Effective Depth of UV-C Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Preparation of Microbial Strains and Inoculation in Soy Milk Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Microbiological Analysis of the Samples and Lethality Determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Determination of the Inactivation Kinetics and Effect of Treatment Variables Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Cleaning and Disinfection of the UV Equipment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: Statistical Analysis of the Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Children: Item: Title: Effect of UV-C Treatments on the Inoculated Microorganism Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Characterization and Evaluation of the UV-C Reactor Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: Mathematical Modelling of UV-C Treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Inactivation Kinetics and Calculation of the 4Duv-c Value for UV-C Treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Info: Title: Drastic Microbial Count Reduction in Soy Milk Using Continuous Short-Wave Ultraviolet Treatments in a Tubular Annular Thin Film UV-C Reactor Author: María Martínez-García, Jezer N. Sauceda-Gálvez, Idoia Codina-Torrella, María Manuela Hernández-Herrero, Ramón Gervilla and Artur X. Roig-Sagués Subject: Vegetative cells of Listeria monocytogenes and Escherichia coli and spores of Bacillus subtilis and Aspergillus niger were inoculated in soy milk at an initial concentration of 5 log CFU/mL. Inoculated and control (non-inoculated) soy milk samples were submitted to three types of treatments using a tubular annular thin film short-wave ultraviolet (UV-C) reactor with 1 mm of layer thickness. Treatments applied depended on the flow rate and the number of entries to the reactor, with UV-C doses ranging from 20 to 160 J/mL. The number of entries into the reactor tube (NET) was established as the most determining parameter for the efficiency of the UV-C treatments. Conidiospores of A. niger were reported as the most resistant, followed by B. subtilis spores, while vegetative cells were the most sensible to UV-C, with Listeria monocytogenes being more sensible than Escherichia coli. Treatments of just 80 J/mL were needed to achieve a 5 log CFU/mL reduction of L. monocytogenes while 160 J/mL was necessary to achieve a similar reduction for A. niger spores. 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