d841f52d2246076a73f6b80660d145e7 pmc_10624686.pdf e1a73593e68b83df23b41f466cb975c6e3749b5e pmc_10624686.pdf 7ec13f8fc687a7766e2f381687596eb671e146c59f77ff0d70d26f8afb682b6b pmc_10624686.pdf Title: Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers Subject: Scientific Reports, https://doi.org/10.1038/s41598-023-39874-0 Author: Verónica Pablo-Fontecha Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Sat Oct 28 11:10:21 2023 CEST ModDate: Fri Nov 3 10:59:28 2023 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2308961 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- UQLFJC+Wingdings-Regular Type 1C WinAnsi yes yes yes 409 0 MinionPro-Regular Type 1C Custom yes no yes 40 0 Corbel-BoldItalic TrueType WinAnsi yes no yes 410 0 NUATED+Corbel-Bold CID TrueType Identity-H yes yes yes 44 0 Corbel2 TrueType WinAnsi yes no yes 45 0 YXORCH+Corbel CID TrueType Identity-H yes yes yes 46 0 Corbel3 CID TrueType Identity-H yes no yes 444 0 MinionPro-Bold Type 1C Custom yes no yes 41 0 MinionPro-It Type 1C WinAnsi yes no yes 42 0 MZEFLF+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 43 0 Corbel4 TrueType WinAnsi yes no yes 74 0 TimesNewRomanPSMT2 TrueType WinAnsi yes no yes 228 0 VZMBHN+MinionPro-Regular2 Type 1C Custom yes yes no 229 0 WDWHVL+MinionPro-It2 Type 1C Custom yes yes no 230 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-03-12 02:09:55 CET RepresentationInformation: pmc_10624686.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-03-12 02:03:43 CET Size: 2308961 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 482 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers Children: Item: Title: Results Children: Item: Title: Selection of pathogenic variants in normal colonic mucosa Item: Title: Sensitivity and selectivity of SuperSelective primers Item: Title: Validation of low represented variants in normal colonic mucosa using SuperSelective primers by ddPCR Item: Title: Discussion Item: Title: Materials and methods Children: Item: Title: Primary samples and nucleic acid extraction Item: Title: Whole-exome sequencing and criteria for SNV selection Item: Title: Control template generation Children: Item: Title: Cloning in plasmid vectors Item: Title: Site-directed mutagenesis by PCR Item: Title: SuperSelective primer design Item: Title: Droplet digital PCR assays Item: Title: qPCR assays Item: Title: References Item: Title: Acknowledgements Info: Title: Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers Author: Verónica Pablo-Fontecha Subject: Scientific Reports, https://doi.org/10.1038/s41598-023-39874-0 Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Sat Oct 28 11:40:21 CEST 2023 ModDate: Fri Nov 03 10:59:28 CET 2023 ID: 0xeed0a6d2776a619916f83fc3a65b1d98, 0xbc83e301b08aec38969ddf70794e2f04 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2032 ImageHeight: 1348 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1418 ImageHeight: 2150 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1772 ImageHeight: 2197 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: MZEFLF+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: NUATED+Corbel-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: YXORCH+Corbel Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: VZMBHN+MinionPro-Regular2 FontSubset: true FirstChar: 30 LastChar: 33 FontDescriptor: FontName: VZMBHN+MinionPro-Regular2 Flags: Symbolic, AllCap FontBBox: 0, -12, 500, 700 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: WDWHVL+MinionPro-It2 FontSubset: true FirstChar: 6 LastChar: 33 FontDescriptor: FontName: WDWHVL+MinionPro-It2 Flags: Symbolic FontBBox: -140, -257, 760, 712 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: UQLFJC+Wingdings-Regular FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: UQLFJC+Wingdings-Regular Flags: Nonsymbolic FontBBox: 0, -103, 1359, 439 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true TrueType: Font: BaseFont: TimesNewRomanPSMT2 FirstChar: 32 LastChar: 118 FontDescriptor: FontName: TimesNewRomanPSMT2 Flags: Serif, Nonsymbolic FontBBox: -568, -307, 2046, 1040 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: Corbel-BoldItalic FirstChar: 0 LastChar: 255 FontDescriptor: FontName: Corbel-BoldItalic Flags: Nonsymbolic, Italic FontBBox: -494, -239, 1194, 952 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: Corbel2 FirstChar: 0 LastChar: 255 FontDescriptor: FontName: Corbel2 Flags: Nonsymbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true CIDFontType2: Font: BaseFont: DTTVOX+Corbel CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: DTTVOX+Corbel Flags: Symbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Font: BaseFont: MZEFLF+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: MZEFLF+TimesNewRomanPSMT Flags: Serif, Symbolic FontBBox: -568, -307, 2046, 1040 FontFile2: true Font: BaseFont: NUATED+Corbel-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: NUATED+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true XMP: 2023-10-28T14:40:21+05:30 Springer 2023-11-03T10:59:28+01:00 2023-11-03T10:59:28+01:00 application/pdf https://doi.org/10.1038/s41598-023-39874-0 Nature Publishing Group UK Scientific Reports, https://doi.org/10.1038/s41598-023-39874-0 Quantification of rare somatic single nucleotide variants by droplet digital PCR using SuperSelective primers Verónica Pablo-Fontecha Eva Hernández-Illán Andrea Reparaz Elena Asensio Jordi Morata Raúl Tonda Sara Lahoz Carolina Parra Juan José Lozano Anabel García-Heredia Alejandro Martínez-Roca Sergi Beltran Francesc Balaguer Rodrigo Jover Antoni Castells Ramon Trullàs Petar Podlesniy Jordi Camps 10.1038/s41598-023-39874-0 2010-04-23 true springer.com springerlink.com https://doi.org/10.1038/s41598-023-39874-0 10.1038/s41598-023-39874-0 2045-2322 journal Scientific Reports The Author(s) 2010-04-23 true 10.1038/s41598-023-39874-0 noindex springer.com springerlink.com VoR uuid:4ab5c275-69c6-47d3-9064-7dbaf2daa215 uuid:ae0c89f7-51fa-41e7-8702-26b9ce06e768 default 1 converted uuid:db98364b-f610-42ab-9f6a-4a033df95727 converted to PDF/A-2b pdfToolbox 2023-10-28T14:41:14+05:30 converted uuid:7503284f-478a-4a91-982c-369bdaea0c10 converted to PDF/A-2b pdfToolbox 2023-10-28T14:41:50+05:30 Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) 2 B http://ns.adobe.com/pdfx/1.3/ pdfx Adobe Document Info PDF eXtension Schema external Mirrors crossmark:MajorVersionDate CrossmarkMajorVersionDate Text external Mirrors crossmark:CrossmarkDomainExclusive CrossmarkDomainExclusive Text internal Mirrors crossmark:DOI doi Text external Mirrors crossmark:CrosMarkDomains CrossMarkDomains seq Text internal A name object indicating whether the document has been modified to include trapping information robots Text internal ID of PDF/X standard GTS_PDFXVersion Text internal Conformance level of PDF/X standard GTS_PDFXConformance Text internal Company creating the PDF Company Text internal Date when document was last modified SourceModified Text http://crossref.org/crossmark/1.0/ crossmark Crossmark Schema internal Usual same as prism:doi DOI Text external The date when a publication was publishe. MajorVersionDate Text internal CrossmarkDomainExclusive CrossmarkDomainExclusive Text internal CrossMarkDomains CrossMarkDomains seq Text http://prismstandard.org/namespaces/basic/2.0/ prism Prism Schema external This element provides the url for an article or unit of content. The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary. url URI external The Digital Object Identifier for the article. The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL). doi Text external ISSN for an electronic version of the issue in which the resource occurs. Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version. issn Text internal Volume number volume Text internal Issue number number Text internal Starting page startingPage Text internal Ending page endingPage Text external The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary. aggregationType Text external Title of the magazine, or other publication, in which a resource was/will be published. Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.” publicationName Text external Copyright copyright Text http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. DocumentID URI internal The common identifier for all versions and renditions of a document. OriginalDocumentID URI internal A reference to the original document from which this one is derived. It is a minimal reference; missing components can be assumed to be unchanged. For example, a new version might only need to specify the instance ID and version number of the previous version, or a rendition might only need to specify the instance ID and rendition class of the original. DerivedFrom ResourceRef Identifies a portion of a document. This can be a position at which the document has been changed since the most recent event history (stEvt:changed). For a resource within an xmpMM:Ingredients list, the ResourceRef uses this type to identify both the portion of the containing document that refers to the resource, and the portion of the referenced resource that is referenced. http://ns.adobe.com/xap/1.0/sType/Part# stPart Part http://www.aiim.org/pdfa/ns/id/ pdfaid PDF/A ID Schema internal Part of PDF/A standard part Integer internal Amendment of PDF/A standard amd Text internal Conformance level of PDF/A standard conformance Text http://ns.adobe.com/xap/1.0/t/pg/ xmpTPg XMP Paged-Text internal XMP08 Spec: An ordered array of plate names that are needed to print the document (including any in contained documents). PlateNames Seq Text http://www.niso.org/schemas/jav/1.0/ jav NISO external Values for Journal Article Version are one of the following: AO = Author’s Original SMUR = Submitted Manuscript Under Review AM = Accepted Manuscript P = Proof VoR = Version of Record CVoR = Corrected Version of Record EVoR = Enhanced Version of Record journal_article_version Closed Choice of Text Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 387, 256, 390, 244 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 437, 246, 440, 234 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 241, 226, 243, 214 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 245, 226, 247, 214 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 509, 196, 512, 184 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 515, 196, 518, 184 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 481, 675, 556, 658 Flags: Print AppearanceDictionary: true Thumb: true Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 371, 714, 373, 702 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 208, 704, 211, 692 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 546, 704, 552, 692 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 261, 654, 266, 642 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 268, 654, 273, 642 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 273, 624, 279, 612 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 280, 624, 286, 612 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 279, 544, 285, 532 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 442, 534, 447, 522 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 449, 534, 454, 522 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 351, 504, 357, 492 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 358, 504, 364, 492 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 367, 504, 373, 492 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 452, 322, 457, 310 Flags: Print AppearanceDictionary: true Destination: 3 Thumb: true Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 289, 311, 294, 299 Flags: Print AppearanceDictionary: true Destination: 59 Annotation: Subtype: Link Rect: 209, 241, 214, 229 Flags: Print AppearanceDictionary: true Destination: 78 Annotation: Subtype: Link Rect: 421, 231, 426, 219 Flags: Print AppearanceDictionary: true Destination: 93 Thumb: true Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 200, 167, 204, 155 Flags: Print AppearanceDictionary: true Destination: 101 Annotation: Subtype: Link Rect: 224, 167, 228, 155 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 506, 127, 510, 115 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 302, 87, 306, 75 Flags: Print AppearanceDictionary: true Destination: 145 Thumb: true Page: Sequence: 5 Thumb: true Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 430, 571, 434, 559 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 540, 541, 544, 529 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 333, 471, 338, 459 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 365, 461, 369, 449 Flags: Print AppearanceDictionary: true Destination: 145 Annotation: Subtype: Link Rect: 461, 419, 467, 407 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 468, 419, 474, 407 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 475, 419, 481, 407 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 221, 379, 226, 367 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 228, 379, 233, 367 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 225, 359, 230, 347 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 232, 359, 238, 347 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 222, 339, 228, 327 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 281, 259, 286, 247 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 194, 189, 200, 177 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 482, 189, 488, 177 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 489, 189, 495, 177 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 218, 179, 223, 167 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 469, 169, 474, 157 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 223, 129, 228, 117 Flags: Print AppearanceDictionary: true Destination: 202 Thumb: true Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 195, 68, 200, 56 Flags: Print AppearanceDictionary: true Destination: 202 Annotation: Subtype: Link Rect: 203, 68, 209, 56 Flags: Print AppearanceDictionary: true Destination: 245 Thumb: true Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 537, 674, 543, 662 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 546, 674, 552, 662 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 203, 450, 209, 438 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 546, 450, 552, 438 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 436, 440, 441, 428 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 244, 430, 250, 418 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 409, 420, 415, 408 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 351, 390, 357, 378 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 467, 370, 472, 358 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 528, 370, 533, 358 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 189, 360, 194, 348 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 261, 360, 267, 348 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 373, 360, 379, 348 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 271, 350, 277, 338 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 404, 310, 410, 298 Flags: Print AppearanceDictionary: true Destination: 245 Annotation: Subtype: Link Rect: 210, 166, 216, 154 Flags: Print AppearanceDictionary: true Destination: 245 Thumb: true Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 365, 480, 369, 468 Flags: Print AppearanceDictionary: true Destination: 59 Annotation: Subtype: Link Rect: 487, 68, 491, 56 Flags: Print AppearanceDictionary: true Destination: 59 Thumb: true Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 484, 580, 554, 568 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 155, 570, 269, 558 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 532, 448, 554, 438 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 169, 439, 281, 429 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 520, 278, 554, 268 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 169, 269, 269, 259 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 458, 116, 544, 106 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 332, 65, 435, 55 Flags: Print AppearanceDictionary: true Thumb: true Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 429, 475, 512, 463 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 422, 315, 554, 303 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 155, 305, 164, 293 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 499, 235, 554, 222 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 155, 224, 260, 212 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 351, 195, 443, 182 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 358, 84, 517, 72 Flags: Print AppearanceDictionary: true Thumb: true Checksum: ea754dc7 Type: CRC32 Checksum: d841f52d2246076a73f6b80660d145e7 Type: MD5 Checksum: e1a73593e68b83df23b41f466cb975c6e3749b5e Type: SHA-1 Checksum: 7ec13f8fc687a7766e2f381687596eb671e146c59f77ff0d70d26f8afb682b6b Type: SHA-256