d890ead07ece35e75a445164e1c5dda0 pmc_10741101.pdf 6c33f284e1b548efa9d60b5554e07f9f4136168f pmc_10741101.pdf 3e0b956cf308fc79a28b79508b169a89decfbd751bcbee1dae16e8010b590123 pmc_10741101.pdf Title: Saliva Sampling Material Matters: Effects on the Results of Saliva Analysis in Pigs Subject: The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13 analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration. The polypropylene sponge provided a higher sample volume than the cotton roll. Although the results of some salivary analytes were equivalent for both materials, other analytes, such as creatine kinase, haptoglobin and total proteins, showed significant differences depending on the material used for saliva collection. Therefore, the type of material used for salivary collection in pigs should be considered when interpreting the results of analyses of the salivary analytes. Keywords: analytes; collection material; pig; stimulated saliva Author: Alba Ortín-Bustillo, María Botía, Marina López-Arjona, Luis Pardo-Marín, José J. Cerón, Silvia Martínez-Subiela, María José López-Martínez, Asta Tvarijonaviciute, Alberto Muñoz-Prieto, Camila P. Rubio, Silvia Martínez-Miró, Damián Escribano and Fernando Tecles Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Dec 5 14:27:19 2023 CET ModDate: Tue Dec 5 14:31:46 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2941983 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 10 0 XIEUDY+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 UFBSZC+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 HAWNPJ+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 UWJUYN+CMSY10 Type 1 Builtin yes yes no 71 0 ADIHBI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 85 0 ADIHBJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 88 0 ADIHBK+PalatinoLinotype TrueType WinAnsi yes yes no 94 0 ADIHCL+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 97 0 ADIHCM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 103 0 ADIIJE+PalatinoLinotype TrueType MacRoman yes yes no 106 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 117 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 121 0 HNPBMK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 163 0 HNPBMM+PalatinoLinotype TrueType WinAnsi yes yes no 166 0 HNPBMO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 169 0 ADIHBI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 214 0 ADIHBK+PalatinoLinotype TrueType WinAnsi yes yes no 217 0 ADIHCM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 220 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-01-11 02:29:39 CET RepresentationInformation: pmc_10741101.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-01-09 02:28:44 CET Size: 2941983 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 385 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: Saliva Sampling Destination: subsection.2.2 Item: Title: Validation of the Sampling Procedure Used for the Comparison of the Materials Destination: subsection.2.3 Item: Title: Comparison between Cotton versus Polypropylene for Saliva Collection Destination: subsection.2.4 Item: Title: Measurements Destination: subsection.2.5 Children: Item: Title: Stress Biomarkers Destination: subsubsection.2.5.1 Item: Title: Immune System and Inflammation Biomarkers Destination: subsubsection.2.5.2 Item: Title: Sepsis Biomarkers Destination: subsubsection.2.5.3 Item: Title: Redox Biomarkers Destination: subsubsection.2.5.4 Item: Title: Analytes Related to Metabolism and Status of Different Tissues and Organs Destination: subsubsection.2.5.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Saliva Sampling Material Matters: Effects on the Results of Saliva Analysis in Pigs Author: Alba Ortín-Bustillo, María Botía, Marina López-Arjona, Luis Pardo-Marín, José J. Cerón, Silvia Martínez-Subiela, María José López-Martínez, Asta Tvarijonaviciute, Alberto Muñoz-Prieto, Camila P. Rubio, Silvia Martínez-Miró, Damián Escribano and Fernando Tecles Subject: The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13 analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration. The polypropylene sponge provided a higher sample volume than the cotton roll. Although the results of some salivary analytes were equivalent for both materials, other analytes, such as creatine kinase, haptoglobin and total proteins, showed significant differences depending on the material used for saliva collection. Therefore, the type of material used for salivary collection in pigs should be considered when interpreting the results of analyses of the salivary analytes. 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