ef0475dd5736d38565b45c9adae311f5 pmc_10747942.pdf a3636e6a5d6cf758c269c52aa04c0dc8d9847e4a pmc_10747942.pdf 59ebf3f2e8c167c56a0a9e0a5520ab0170f2a489cc85da97dc1a45cebf73ed2e pmc_10747942.pdf Title: Enhanced Susceptibility to Tomato Chlorosis Virus (ToCV) in Hsp90- and Sgt1-Silenced Plants: Insights from Gene Expression Dynamics Subject: The emerging whitefly-transmitted crinivirus tomato chlorosis virus (ToCV) causes substantial economic losses by inducing yellow leaf disorder in tomato crops. This study explores potential resistance mechanisms by examining early-stage molecular responses to ToCV. A time-course transcriptome analysis compared naïve, mock, and ToCV-infected plants at 2, 7, and 14 days post-infection (dpi). Gene expression changes were most notable at 2 and 14 dpi, likely corresponding to whitefly feeding and viral infection. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed key genes and pathways associated with ToCV infection, including those related to plant immunity, flavonoid and steroid biosynthesis, photosynthesis, and hormone signaling. Additionally, virus-derived small interfering RNAs (vsRNAs) originating from ToCV predominantly came from RNA2 and were 22 nucleotides in length. Furthermore, two genes involved in plant immunity, Hsp90 (heat shock protein 90) and its co-chaperone Sgt1 (suppressor of the G2 allele of Skp1) were targeted through viral-induced gene silencing (VIGS), showing a potential contribution to basal resistance against viral infections since their reduction correlated with increased ToCV accumulation. This study provides insights into tomato plant responses to ToCV, with potential implications for developing effective disease control strategies. Keywords: basal resistance; Hsp90; Sgt1; ToCV; Bemisia tabaci; tomato Author: Irene Ontiveros, Noé Fernández-Pozo, Anna Esteve-Codina, Juan José López-Moya and Juan Antonio Díaz-Pendón Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Dec 1 05:00:22 2023 CET ModDate: Fri Dec 1 05:05:26 2023 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2377708 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- GCRDPW+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 EPUDDC+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 QNCZHJ+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 LVZEVR+URWPalladioL-BoldItal Type 1 Custom yes yes no 23 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 27 0 HOWKDX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 46 0 YUWHZX+CMSY10 Type 1 Builtin yes yes no 68 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 72 0 YWQWLZ+VnURWPalladioL Type 1 Custom yes yes no 79 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 89 0 EKMFDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 103 0 EKMFDO+PalatinoLinotype CID TrueType Identity-H yes yes yes 106 0 EKMFDP+PalatinoLinotype TrueType WinAnsi yes yes no 112 0 EKMFEB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 115 0 EKMFPP+PalatinoLinotype TrueType MacRoman yes yes no 118 0 EKMFDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 132 0 EKMFDO+PalatinoLinotype CID TrueType Identity-H yes yes yes 135 0 EKMFDP+PalatinoLinotype TrueType WinAnsi yes yes no 141 0 EKMFEB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 144 0 EKMFPP+PalatinoLinotype TrueType MacRoman yes yes no 147 0 EKMFDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 162 0 EKMFDO+PalatinoLinotype CID TrueType Identity-H yes yes yes 165 0 EKMFDP+PalatinoLinotype TrueType WinAnsi yes yes no 171 0 EKMFEB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 174 0 EKMFPP+PalatinoLinotype TrueType MacRoman yes yes no 177 0 EKMFDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 191 0 EKMFDO+PalatinoLinotype CID TrueType Identity-H yes yes yes 194 0 EKMFDP+PalatinoLinotype TrueType WinAnsi yes yes no 200 0 EKMFEB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 203 0 EKMFPP+PalatinoLinotype TrueType MacRoman yes yes no 206 0 EKMFDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 220 0 EKMFDO+PalatinoLinotype CID TrueType Identity-H yes yes yes 223 0 EKMFDP+PalatinoLinotype TrueType WinAnsi yes yes no 229 0 EKMFEB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 232 0 EKMFPP+PalatinoLinotype TrueType MacRoman yes yes no 235 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-07-17 02:36:30 CEST RepresentationInformation: pmc_10747942.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-07-12 02:35:34 CEST Size: 2377708 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 555 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Plant Material and Virus Infection Destination: subsection.2.1 Item: Title: Sample Collection and RNA Extraction Destination: subsection.2.2 Item: Title: Libraries Construction and Sequencing Destination: subsection.2.3 Item: Title: RNA-Seq and sRNA Data Analysis Destination: subsection.2.4 Item: Title: Virus-Induced Gene Silencing Destination: subsection.2.5 Item: Title: Quantitative Real-Time PCR Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Identification of DEGs in Tomato Leaves at Different Stages of ToCV Infection Destination: subsection.3.1 Item: Title: Gene Ontology Enrichment Analysis in Response to ToCV Destination: subsection.3.2 Item: Title: Impacted Pathways in ToCV Infection Destination: subsection.3.3 Item: Title: Characterizing Viral Small RNAs in ToCV-Infected Plants Destination: subsection.3.4 Item: Title: Enhanced ToCV Susceptibility in Tomato Plants after Silencing of Hsp90 and Sgt1 Genes Destination: subsection.3.5 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: section.A. Info: Title: Enhanced Susceptibility to Tomato Chlorosis Virus (ToCV) in Hsp90- and Sgt1-Silenced Plants: Insights from Gene Expression Dynamics Author: Irene Ontiveros, Noé Fernández-Pozo, Anna Esteve-Codina, Juan José López-Moya and Juan Antonio Díaz-Pendón Subject: The emerging whitefly-transmitted crinivirus tomato chlorosis virus (ToCV) causes substantial economic losses by inducing yellow leaf disorder in tomato crops. This study explores potential resistance mechanisms by examining early-stage molecular responses to ToCV. A time-course transcriptome analysis compared naïve, mock, and ToCV-infected plants at 2, 7, and 14 days post-infection (dpi). Gene expression changes were most notable at 2 and 14 dpi, likely corresponding to whitefly feeding and viral infection. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed key genes and pathways associated with ToCV infection, including those related to plant immunity, flavonoid and steroid biosynthesis, photosynthesis, and hormone signaling. Additionally, virus-derived small interfering RNAs (vsRNAs) originating from ToCV predominantly came from RNA2 and were 22 nucleotides in length. Furthermore, two genes involved in plant immunity, Hsp90 (heat shock protein 90) and its co-chaperone Sgt1 (suppressor of the G2 allele of Skp1) were targeted through viral-induced gene silencing (VIGS), showing a potential contribution to basal resistance against viral infections since their reduction correlated with increased ToCV accumulation. This study provides insights into tomato plant responses to ToCV, with potential implications for developing effective disease control strategies. 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Link Rect: 415, 169, 451, 180 Annotation: Subtype: Link Rect: 439, 135, 476, 146 Annotation: Subtype: Link Rect: 312, 112, 350, 123 Annotation: Subtype: Link Rect: 396, 89, 434, 100 Annotation: Subtype: Link Rect: 180, 66, 217, 77 Page: Sequence: 16 Annotations: Annotation: Subtype: Link Rect: 59, 711, 96, 721 Annotation: Subtype: Link Rect: 510, 687, 547, 698 Annotation: Subtype: Link Rect: 520, 676, 557, 687 Annotation: Subtype: Link Rect: 59, 665, 95, 675 Annotation: Subtype: Link Rect: 519, 641, 557, 652 Annotation: Subtype: Link Rect: 59, 630, 95, 640 Annotation: Subtype: Link Rect: 344, 607, 382, 617 Annotation: Subtype: Link Rect: 388, 607, 424, 617 Annotation: Subtype: Link Rect: 443, 583, 481, 594 Annotation: Subtype: Link Rect: 131, 550, 168, 560 Checksum: fb0f9bd3 Type: CRC32 Checksum: ef0475dd5736d38565b45c9adae311f5 Type: MD5 Checksum: a3636e6a5d6cf758c269c52aa04c0dc8d9847e4a Type: SHA-1 Checksum: 59ebf3f2e8c167c56a0a9e0a5520ab0170f2a489cc85da97dc1a45cebf73ed2e Type: SHA-256