87e4fb2e7715913155d2ce50904c85c0 pmc_10747959.pdf 3c1d4309c4d8c26a2720fbca8916c2b98e354c7d pmc_10747959.pdf 636f99c597e2c2ca0642abef9bfd7aa447b7c2ed8efc5e8fc43e03e0ea09af66 pmc_10747959.pdf Title: Gastroprotective Effects of Oral Glycosaminoglycans with Sodium Alginate in an Indomethacin-Induced Gastric Injury Model in Rats Subject: The gastrointestinal (GI) mucosal barrier is often exposed to inflammatory and erosive insults, resulting in gastric lesions. Glycosaminoglycans (GAGs), such as hyaluronic acid (HA), chondroitin sulfate (CS), and N-acetylglucosamine (NAG) have shown potential beneficial effects as GI protectants. This study aimed to evaluate the gastroprotective effects of oral GAGs in rats with indomethacin-induced GI lesions. Forty-five Sprague–Dawley rats (8–9 weeks-old, 228 7 g) were included in the study, divided into five study groups, and given, administered orally, either sucralfate (positive control group; PC), NAG (G group), sodium alginate plus HA and CS (AHC group), sodium alginate plus HA, CS, and NAG (AHCG group), or no treatment (negative control group; NC). Animals were administered 12.5 mg/kg indomethacin orally 15 min after receiving the assigned treatment. After 4 h, stomach samples were obtained and used to perform a macroscopic evaluation of gastric lesions and to allow histological assessment of the gastric wall (via H/E staining) and mucous (via PAS staining). The AHCG group showed significant gastroprotective improvements compared to the NC group, and a similar efficacy to the PC group. This combination of sodium alginate with GAGs might, therefore, become a safe and effective alternative to prescription drugs for gastric lesions, such as sucralfate, and have potential usefulness in companion animals. Keywords: glycosaminoglycans; gastroprotection; indomethacin; chondroitin; hyaluronate; dermatan sulfate; sucralfate; sodium alginate; N-acetylglucosamine Author: Sara Traserra, Héctor Cuerda, Adriana Vallejo, Sergi Segarra, Roger Sabata and Marcel Jimenez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Nov 27 11:42:56 2023 CET ModDate: Mon Nov 27 11:49:01 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5689246 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WDGVSG+VnURWPalladioL Type 1 Custom yes yes no 10 0 MXHPFS+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 IFCDMM+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 MIZXKD+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 28 0 BTFGED+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 CTCXDQ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 CAMXTK+EURM10 Type 1 Builtin yes yes no 75 0 ZPGVCH+CMEX10 Type 1 Builtin yes yes no 82 0 JHYTSG+CMR10 Type 1 Builtin yes yes no 86 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 103 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 106 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 109 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 143 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 146 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 149 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 184 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 187 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 190 0 EMGGLL+PalatinoLinotype CID TrueType Identity-H yes yes yes 208 0 EMHCEN+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 214 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 217 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 220 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 223 0 EMGGLL+PalatinoLinotype CID TrueType Identity-H yes yes yes 245 0 EMHCEN+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 251 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 254 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 257 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 260 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 295 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 298 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 301 0 EMGGMM+PalatinoLinotype TrueType WinAnsi yes yes no 339 0 EMGGMO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 342 0 EMGGNA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 345 0 IOLAWT+PazoMath-Italic Type 1 Builtin yes yes no 360 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-02-02 02:32:24 CET RepresentationInformation: pmc_10747959.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-01-09 02:28:35 CET Size: 5689246 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 519 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: Prototype Administration and Induction of Gastric Lesions Destination: subsection.2.2 Item: Title: Drugs Destination: subsection.2.3 Item: Title: Macroscopic Evaluation Destination: subsection.2.4 Item: Title: Microscopic Evaluation Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Establishment of an In Vivo Model of Indomethacin-Induced Gastric Damage Destination: subsection.3.1 Item: Title: Macroscopic Results Destination: subsection.3.2 Item: Title: Microscopic Results Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Gastroprotective Effects of Oral Glycosaminoglycans with Sodium Alginate in an Indomethacin-Induced Gastric Injury Model in Rats Author: Sara Traserra, Héctor Cuerda, Adriana Vallejo, Sergi Segarra, Roger Sabata and Marcel Jimenez Subject: The gastrointestinal (GI) mucosal barrier is often exposed to inflammatory and erosive insults, resulting in gastric lesions. Glycosaminoglycans (GAGs), such as hyaluronic acid (HA), chondroitin sulfate (CS), and N-acetylglucosamine (NAG) have shown potential beneficial effects as GI protectants. This study aimed to evaluate the gastroprotective effects of oral GAGs in rats with indomethacin-induced GI lesions. Forty-five Sprague Dawley rats (8 9 weeks-old, 228 7 g) were included in the study, divided into five study groups, and given, administered orally, either sucralfate (positive control group; PC), NAG (G group), sodium alginate plus HA and CS (AHC group), sodium alginate plus HA, CS, and NAG (AHCG group), or no treatment (negative control group; NC). Animals were administered 12.5 mg/kg indomethacin orally 15 min after receiving the assigned treatment. After 4 h, stomach samples were obtained and used to perform a macroscopic evaluation of gastric lesions and to allow histological assessment of the gastric wall (via H/E staining) and mucous (via PAS staining). The AHCG group showed significant gastroprotective improvements compared to the NC group, and a similar efficacy to the PC group. This combination of sodium alginate with GAGs might, therefore, become a safe and effective alternative to prescription drugs for gastric lesions, such as sucralfate, and have potential usefulness in companion animals. Keywords: glycosaminoglycans; gastroprotection; indomethacin; chondroitin; hyaluronate; dermatan sulfate; sucralfate; sodium alginate; N-acetylglucosamine Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Nov 27 11:42:56 CET 2023 ModDate: Mon Nov 27 11:49:01 CET 2023 ID: 0xd88bb076ffac943c8835c8c520ead67d, 0xd88bb076ffac943c8835c8c520ead67d Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: BTFGED+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 178 FontDescriptor: FontName: BTFGED+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: ZPGVCH+CMEX10 FontSubset: true FirstChar: 18 LastChar: 19 FontDescriptor: FontName: ZPGVCH+CMEX10 Flags: Symbolic FontBBox: -24, -2960, 1454, 772 FontFile: true Font: BaseFont: IFCDMM+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 241 FontDescriptor: FontName: IFCDMM+URWPalladioL-Roma Flags: Symbolic FontBBox: 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