d16981f2277513efdbe9ba1eaf4786d8 pmc_10756948.pdf e72a107b05833e4f864e4fb110306ddffd0fedac pmc_10756948.pdf 3ff9b8ed22227434658fcc98cdb4bf3879c67feb054c8819732154bcffd362ef pmc_10756948.pdf Title: High‐throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome Creator: Adobe InDesign 15.1 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Thu Dec 28 09:33:09 2023 CET ModDate: Thu Dec 28 09:33:10 2023 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 844283 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- KRTQWO+Lato-Regular CID TrueType Identity-H yes yes yes 443 0 YZUWKM+Lato-Bold CID TrueType Identity-H yes yes yes 444 0 MCVCYK+STIX-Regular Type 1C Custom yes yes yes 445 0 QAVACC+Lato-Italic TrueType WinAnsi yes yes yes 446 0 EIWGQA+Lato-Regular TrueType WinAnsi yes yes yes 447 0 TPHSSW+Lato-Bold TrueType WinAnsi yes yes yes 448 0 EIWGQA+Lato-Regular TrueType WinAnsi yes yes yes 263 0 IUYKZS+Lato-Regular-SC700 TrueType WinAnsi yes yes yes 264 0 EIWGQA+Lato-Regular TrueType WinAnsi yes yes yes 265 0 IUYKZS+Lato-Regular-SC700 TrueType WinAnsi yes yes yes 266 0 GTTSSW+STIX-Italic Type 1C Custom yes yes yes 70 0 DRFACC+HelveticaLTStd-Bold Type 1C WinAnsi yes yes no 71 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-05-01 02:42:38 CEST RepresentationInformation: pmc_10756948.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-04-24 02:38:20 CEST Size: 844283 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 476 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: High-throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome Children: Item: Title: Abstract Item: Title: 1|INTRODUCTION Item: Title: 2|MATERIALS AND METHODS Children: Item: Title: 2.1|Patients and samples Item: Title: 2.2|Cell isolation and RNA extraction Item: Title: 2.3|High-throughput RNA sequencing Item: Title: 2.4|Sequence analysis, definitions, and interpretation Item: Title: 2.5|Comparison of CDR3 sequences Item: Title: 2.6|High-resolution HLA typing Item: Title: 2.7|Statistical analysis Item: Title: 3|RESULTS Children: Item: Title: 3.1|Highly expanded predominant TRA and TRB CDR3 regions only found in SS Item: Title: 3.2|CDR3 regions almost identical to the predominant in the same SS patient Item: Title: 3.3|HLA similarities between SS patients Item: Title: 3.4|Shared CDR3 regions between distinct patients Item: Title: 3.5|Antigen matches mainly detected for shared CDR3 regions Item: Title: 4|DISCUSSION Item: Title: AUTHOR CONTRIBUTIONS Item: Title: ACKNOWLEDGMENTS Item: Title: CONFLICT OF INTEREST STATEMENT Item: Title: REFERENCES Info: Title: High‐throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome Creator: Adobe InDesign 15.1 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Thu Dec 28 10:03:09 CET 2023 ModDate: Thu Dec 28 10:03:10 CET 2023 ID: 0x4ee67342c22e0a41bd51f21abf533058, 0x7965a9f863b38409c823ac7781d5cb0b Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 47 ImageHeight: 43 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 48 ImageHeight: 41 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 47 ImageHeight: 43 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 48 ImageHeight: 41 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 408 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 408 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 4 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 4 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 409 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 409 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 3 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 411 ImageHeight: 3 BitsPerSample: 8 BitsPerSampleUnit: integer ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 264 ImageHeight: 333 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode ColorSpace: Separation Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 264 ImageHeight: 333 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode ColorSpace: Separation Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: KRTQWO+Lato-Regular Encoding: Identity-H ToUnicode: true Font: BaseFont: YZUWKM+Lato-Bold Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: GTTSSW+STIX-Italic FontSubset: true FirstChar: 31 LastChar: 31 FontDescriptor: FontName: GTTSSW+STIX-Italic Flags: Serif, Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: DRFACC+HelveticaLTStd-Bold FontSubset: true FirstChar: 40 LastChar: 70 FontDescriptor: FontName: DRFACC+HelveticaLTStd-Bold Flags: Nonsymbolic FontBBox: -170, -228, 1003, 962 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: MCVCYK+STIX-Regular FontSubset: true FirstChar: 29 LastChar: 62 FontDescriptor: FontName: MCVCYK+STIX-Regular Flags: Serif, Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true TrueType: Font: BaseFont: TPHSSW+Lato-Bold FontSubset: true FirstChar: 32 LastChar: 243 FontDescriptor: FontName: TPHSSW+Lato-Bold Flags: Nonsymbolic FontBBox: -603, -285, 1344, 1100 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: QAVACC+Lato-Italic FontSubset: true FirstChar: 32 LastChar: 243 FontDescriptor: FontName: QAVACC+Lato-Italic Flags: Nonsymbolic, Italic FontBBox: -501, -268, 1290, 1077 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: EIWGQA+Lato-Regular FontSubset: true FirstChar: 32 LastChar: 243 FontDescriptor: FontName: EIWGQA+Lato-Regular Flags: Nonsymbolic FontBBox: -546, -268, 1342, 1079 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true CIDFontType2: Font: BaseFont: KRTQWO+Lato-Regular CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: KRTQWO+Lato-Regular Flags: Symbolic FontBBox: -546, -268, 1342, 1079 FontFile2: true Font: BaseFont: YZUWKM+Lato-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: YZUWKM+Lato-Bold Flags: Symbolic FontBBox: -603, -285, 1344, 1100 FontFile2: true XMP: VoR 10.1002/jcla.24982 10.1002/jcla.24982 10.1002/(ISSN)1098-2825 3 High‐throughput RNA sequencing of the T cell receptor alpha and beta chains for simultaneous clonality and biological analyses in Sezary syndrome Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 45, 25, 155, 16 Annotation: Subtype: Link Rect: 410, 34, 516, 25 Annotation: Subtype: Link Rect: 128, 585, 147, 569 ActionDest: 139 Annotation: Subtype: Link Rect: 147, 583, 160, 570 Annotation: Subtype: Link Rect: 293, 585, 305, 569 ActionDest: 139 Annotation: Subtype: Link Rect: 305, 583, 317, 570 Annotation: Subtype: Link Rect: 421, 585, 433, 569 ActionDest: 139 Annotation: Subtype: Link Rect: 433, 583, 445, 570 Annotation: Subtype: Link Rect: 161, 567, 180, 551 ActionDest: 139 Annotation: Subtype: Link Rect: 180, 565, 193, 552 Annotation: Subtype: Link Rect: 288, 567, 301, 551 ActionDest: 139 Annotation: Subtype: Link Rect: 301, 565, 313, 552 Annotation: Subtype: Link Rect: 461, 567, 465, 551 ActionDest: 139 Annotation: Subtype: Link Rect: 465, 565, 478, 552 Annotation: Subtype: Link Rect: 123, 549, 127, 533 ActionDest: 139 Annotation: Subtype: Link Rect: 127, 547, 140, 534 Annotation: Subtype: Link Rect: 276, 549, 286, 533 ActionDest: 139 Annotation: Subtype: Link Rect: 286, 547, 298, 534 Annotation: Subtype: Link Rect: 419, 549, 429, 533 ActionDest: 139 Annotation: Subtype: Link Rect: 429, 547, 441, 534 Annotation: Subtype: Link Rect: 131, 531, 141, 515 ActionDest: 139 Annotation: Subtype: Link Rect: 141, 529, 153, 516 Annotation: Subtype: Link Rect: 260, 531, 281, 515 ActionDest: 139 Annotation: Subtype: Link Rect: 281, 529, 294, 516 Annotation: Subtype: Link Rect: 385, 531, 390, 515 ActionDest: 139 Annotation: Subtype: Link Rect: 390, 529, 403, 516 Annotation: Subtype: Link Rect: 499, 531, 512, 515 ActionDest: 139 Annotation: Subtype: Link Rect: 512, 529, 524, 516 Annotation: Subtype: Link Rect: 133, 513, 177, 497 ActionDest: 139 Annotation: Subtype: Link Rect: 177, 511, 189, 498 Annotation: Subtype: Link Rect: 306, 513, 306, 497 Annotation: Subtype: Link Rect: 306, 513, 318, 497 ActionDest: 139 Annotation: Subtype: Link Rect: 318, 511, 330, 498 Annotation: Subtype: Link Rect: 66, 433, 160, 424 Annotation: Subtype: Link Rect: 211, 74, 395, 65 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 116, 536, 120, 526 ActionDest: 159 Annotation: Subtype: Link Rect: 118, 432, 127, 422 ActionDest: 159 Annotation: Subtype: Link Rect: 76, 380, 86, 370 ActionDest: 159 Annotation: Subtype: Link Rect: 63, 328, 66, 318 ActionDest: 159 Annotation: Subtype: Link Rect: 224, 315, 228, 305 ActionDest: 159 Annotation: Subtype: Link Rect: 124, 250, 128, 240 ActionDest: 159 Annotation: Subtype: Link Rect: 155, 224, 167, 214 ActionDest: 159 Annotation: Subtype: Link Rect: 67, 211, 83, 201 ActionDest: 159 Annotation: Subtype: Link Rect: 129, 81, 138, 71 ActionDest: 253 Annotation: Subtype: Link Rect: 328, 588, 337, 578 ActionDest: 253 Annotation: Subtype: Link Rect: 332, 523, 339, 513 ActionDest: 159 Annotation: Subtype: Link Rect: 337, 510, 346, 500 ActionDest: 253 Annotation: Subtype: Link Rect: 362, 289, 369, 279 ActionDest: 159 Annotation: Subtype: Link Rect: 410, 289, 419, 279 ActionDest: 253 Annotation: Subtype: Link Rect: 463, 276, 549, 266 Annotation: Subtype: Link Rect: 306, 263, 415, 253 Annotation: Subtype: Link Rect: 399, 250, 486, 240 Annotation: Subtype: Link Rect: 543, 237, 549, 227 ActionDest: 159 Annotation: Subtype: Link Rect: 358, 211, 366, 201 ActionDest: 253 Annotation: Subtype: Link Rect: 309, 94, 384, 84 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 94, 445, 174, 435 Annotation: Subtype: Link Rect: 178, 445, 184, 435 ActionDest: 159 Annotation: Subtype: Link Rect: 281, 328, 288, 318 ActionDest: 159 Annotation: Subtype: Link Rect: 376, 328, 385, 318 ActionDest: 253 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 146, 692, 151, 682 ActionDest: 45 Annotation: Subtype: Link Rect: 182, 653, 192, 641 ActionDest: 57 Annotation: Subtype: Link Rect: 194, 432, 204, 420 ActionDest: 57 Annotation: Subtype: Link Rect: 394, 731, 403, 721 ActionDest: 253 Annotation: Subtype: Link Rect: 457, 653, 466, 643 ActionDest: 253 Annotation: Subtype: Link Rect: 443, 575, 453, 563 ActionDest: 57 Annotation: Subtype: Link Rect: 334, 536, 344, 526 ActionDest: 57 Annotation: Subtype: Link Rect: 380, 471, 385, 461 ActionDest: 99 Annotation: Subtype: Link Rect: 354, 354, 360, 344 ActionDest: 159 Annotation: Subtype: Link Rect: 387, 354, 396, 344 ActionDest: 253 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 161, 276, 171, 264 ActionDest: 57 Annotation: Subtype: Link Rect: 111, 133, 120, 123 ActionDest: 253 Annotation: Subtype: Link Rect: 260, 55, 269, 45 ActionDest: 253 Annotation: Subtype: Link Rect: 330, 263, 335, 253 ActionDest: 115 Annotation: Subtype: Link Rect: 522, 224, 531, 212 ActionDest: 57 Annotation: Subtype: Link Rect: 402, 55, 406, 45 ActionDest: 115 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 253, 510, 263, 500 ActionDest: 159 Annotation: Subtype: Link Rect: 212, 341, 215, 331 ActionDest: 159 Annotation: Subtype: Link Rect: 197, 146, 220, 136 ActionDest: 159 Annotation: Subtype: Link Rect: 218, 68, 233, 58 ActionDest: 159 Annotation: Subtype: Link Rect: 543, 471, 549, 461 ActionDest: 159 Annotation: Subtype: Link Rect: 450, 432, 464, 422 ActionDest: 159 Annotation: Subtype: Link Rect: 428, 406, 442, 396 ActionDest: 159 Annotation: Subtype: Link Rect: 422, 328, 436, 318 ActionDest: 159 Annotation: Subtype: Link Rect: 347, 198, 361, 188 ActionDest: 159 Annotation: Subtype: Link Rect: 458, 55, 465, 45 ActionDest: 159 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 160, 692, 174, 682 ActionDest: 159 Annotation: Subtype: Link Rect: 281, 666, 288, 656 ActionDest: 159 Annotation: Subtype: Link Rect: 160, 601, 167, 591 ActionDest: 159 Annotation: Subtype: Link Rect: 205, 419, 220, 409 ActionDest: 253 Annotation: Subtype: Link Rect: 231, 354, 234, 344 ActionDest: 159 Annotation: Subtype: Link Rect: 188, 159, 203, 149 ActionDest: 253 Annotation: Subtype: Link Rect: 215, 146, 230, 136 ActionDest: 253 Annotation: Subtype: Link Rect: 439, 679, 445, 669 ActionDest: 159 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 98, 642, 110, 630 Annotation: Subtype: Link Rect: 110, 640, 257, 630 Annotation: Subtype: Link Rect: 117, 629, 130, 617 Annotation: Subtype: Link Rect: 130, 627, 277, 617 Annotation: Subtype: Link Rect: 99, 616, 111, 604 Annotation: Subtype: Link Rect: 111, 614, 259, 604 Annotation: Subtype: Link Rect: 119, 603, 131, 591 Annotation: Subtype: Link Rect: 131, 601, 278, 591 Annotation: Subtype: Link Rect: 94, 590, 106, 578 Annotation: Subtype: Link Rect: 106, 588, 253, 578 Annotation: Subtype: Link Rect: 126, 577, 138, 565 Annotation: Subtype: Link Rect: 138, 575, 284, 565 Annotation: Subtype: Link Rect: 95, 564, 107, 552 Annotation: Subtype: Link Rect: 107, 562, 254, 552 Annotation: Subtype: Link Rect: 120, 551, 132, 539 Annotation: Subtype: Link Rect: 132, 549, 280, 539 Annotation: Subtype: Link Rect: 110, 538, 123, 526 Annotation: Subtype: Link Rect: 123, 536, 270, 526 Annotation: Subtype: Link Rect: 99, 525, 112, 513 Annotation: Subtype: Link Rect: 112, 523, 259, 513 Annotation: Subtype: Link Rect: 101, 512, 114, 500 Annotation: Subtype: Link Rect: 113, 510, 261, 500 Annotation: Subtype: Link Rect: 91, 499, 103, 487 Annotation: Subtype: Link Rect: 103, 497, 251, 487 Annotation: Subtype: Link Rect: 95, 486, 108, 474 Annotation: Subtype: Link Rect: 108, 484, 256, 474 Annotation: Subtype: Link Rect: 101, 473, 113, 461 Annotation: Subtype: Link Rect: 113, 471, 262, 461 Annotation: Subtype: Link Rect: 108, 460, 121, 448 Annotation: Subtype: Link Rect: 121, 458, 269, 448 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 395, 673, 465, 662 Checksum: aa0f276b Type: CRC32 Checksum: d16981f2277513efdbe9ba1eaf4786d8 Type: MD5 Checksum: e72a107b05833e4f864e4fb110306ddffd0fedac Type: SHA-1 Checksum: 3ff9b8ed22227434658fcc98cdb4bf3879c67feb054c8819732154bcffd362ef Type: SHA-256