2a8f730ec5b5bb1f72bf2275fb756125 pmc_10792485.pdf 32ab85a2a5351c8445b6be78e14aa1bd49693d56 pmc_10792485.pdf ae53ee15cba56d8ad3ec5dbb2674ccf911e115bcabbe1277c07684a1cf329075 pmc_10792485.pdf Title: DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers Subject: Materials Today Bio, 24 (2024) 100900. doi:10.1016/j.mtbio.2023.100900 Keywords: DNA data storage,Nanofibers,Melt-electrowriting,Electrospinning,Additive manufacturing Author: Diana Soukarie Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Sat Jan 13 09:14:35 2024 CET ModDate: Sat Jan 13 17:15:07 2024 CET Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 7395656 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EGMNDK+CharisSIL CID TrueType Identity-H yes yes yes 558 0 BWWBBP+STIX-Regular Type 1C Custom yes yes yes 563 0 LGNXJK+CharisSIL-Italic CID TrueType Identity-H yes yes yes 568 0 PYJLJP+CharisSIL-Bold CID TrueType Identity-H yes yes yes 573 0 QJFZWB+TeX_CM_Roman Type 1C Builtin yes yes yes 578 0 RRFKWB+Univers Type 1C Builtin yes yes yes 582 0 AKEMVM+CharisSIL CID TrueType Identity-H yes yes yes 549 0 CQGNPQ+STIX-Italic Type 1C Custom yes yes yes 226 0 VQXFPH+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 231 0 WIISYJ+CharisSIL-Italic CID TrueType Identity-H yes yes yes 219 0 DXCYOY+STIXGeneral Type 1C Custom yes yes yes 235 0 NTMBZO+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 80 0 KKMYLN+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 91 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-06-03 02:46:04 CEST RepresentationInformation: pmc_10792485.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-06-02 10:33:37 CEST Size: 7395656 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 657 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Results and discussion Destination: mk:H1_2 Children: Item: Title: 2.1 DNA encapsulation in polymer fibers via solution electrospinning Destination: mk:H2_1 Item: Title: 2.2 Controllable retrieval of DNA from electrospun nanofibers Destination: mk:H2_2 Item: Title: 2.3 Encoding and retrieval of message-loaded oligonucleotides Destination: mk:H2_3 Item: Title: 2.4 Accelerated aging tests Destination: mk:H2_4 Item: Title: 2.5 Melt-electrowritten PCL/DNA microfibers Destination: mk:H2_5 Item: Title: 3 Conclusion Destination: mk:H1_3 Item: Title: 4 Materials and methods Destination: mk:H1_4 Children: Item: Title: 4.1 Encoding and oligonucleotide design Destination: mk:H2_6 Item: Title: 4.2 Chemicals Destination: mk:H2_7 Item: Title: 4.3 Polymer/DNA mixture preparation Destination: mk:H2_8 Item: Title: 4.4 Electrospinning Destination: mk:H2_9 Item: Title: 4.5 DNA passive release study with UV spectroscopy Destination: mk:H2_10 Item: Title: 4.6 PCR DNA amplification Destination: mk:H2_11 Item: Title: 4.7 DNA sanger sequencing Destination: mk:H2_12 Item: Title: 4.8 Accelerated aging test Destination: mk:H2_13 Item: Title: 4.9 Melt-electrowriting (MEW) Destination: mk:H2_14 Item: Title: 4.10 Fibers characterization Destination: mk:H2_15 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Data availability Destination: datalink3 Item: Title: Acknowledgements Destination: mk:HH1_4 Item: Title: Appendix A Supplementary data Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers Author: Diana Soukarie Subject: Materials Today Bio, 24 (2024) 100900. doi:10.1016/j.mtbio.2023.100900 Keywords: DNA data storage,Nanofibers,Melt-electrowriting,Electrospinning,Additive manufacturing Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Sat Jan 13 09:14:35 CET 2024 ModDate: Sat Jan 13 17:15:07 CET 2024 ID: 0x6a7808ce483191080065f627d7e2edf3, 0xcad4cfa88092a234856c78f897140a17 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: LGNXJK+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: KKMYLN+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: PYJLJP+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: EGMNDK+CharisSIL Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: NTMBZO+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: NTMBZO+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: QJFZWB+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: QJFZWB+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: CQGNPQ+STIX-Italic FontSubset: true FirstChar: 1 LastChar: 6 FontDescriptor: FontName: CQGNPQ+STIX-Italic Flags: Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: BWWBBP+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 9 FontDescriptor: FontName: BWWBBP+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: RRFKWB+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: RRFKWB+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: VQXFPH+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: VQXFPH+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: DXCYOY+STIXGeneral FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: DXCYOY+STIXGeneral Flags: Nonsymbolic FontBBox: -178, -443, 2000, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: EGMNDK+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: EGMNDK+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: LGNXJK+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: LGNXJK+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: KKMYLN+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: KKMYLN+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: PYJLJP+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: PYJLJP+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true XMP: application/pdf 10.1016/j.mtbio.2023.100900 Elsevier Ltd Materials Today Bio, 24 (2024) 100900. doi:10.1016/j.mtbio.2023.100900 DNA data storage Nanofibers Melt-electrowriting Electrospinning Additive manufacturing DNA data storage in electrospun and melt-electrowritten composite nucleic acid-polymer fibers Diana Soukarie Lluis Nocete Alexander M. Bittner Ibon Santiago journal Materials Today Bio © 2023 The Author(s). Published by Elsevier Ltd. 2590-0064 24 1 February 2024 2024-02-01 100900 100900 10.1016/j.mtbio.2023.100900 https://doi.org/10.1016/j.mtbio.2023.100900 2010-04-23 true 10.1016/j.mtbio.2023.100900 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by-nc-nd/4.0/ VoR 10.1016/j.mtbio.2023.100900 noindex 2010-04-23 true 13th January 2024 sciencedirect.com elsevier.com Elsevier 2024-01-13T16:15:07 2024-01-13T08:14:35 2024-01-13T16:15:07 True 13th January 2024 Acrobat Distiller 8.1.0 (Windows) DNA data storage,Nanofibers,Melt-electrowriting,Electrospinning,Additive manufacturing uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 110, 571, 181, 582 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 181, 571, 292, 582 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 292, 576, 298, 587 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 299, 571, 376, 582 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 376, 576, 382, 587 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 383, 579, 387, 584 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 383, 571, 387, 582 Flags: Print Destination: 534 Annotation: Subtype: Link Rect: 78, 329, 82, 337 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 216, 235, 220, 243 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 66, 224, 70, 232 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 117, 224, 121, 232 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 123, 224, 128, 232 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 200, 193, 204, 201 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 155, 161, 159, 169 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 161, 161, 166, 169 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 547, 350, 552, 358 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 543, 318, 552, 326 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 337, 276, 346, 284 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 379, 255, 388, 263 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 506, 255, 510, 263 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 344, 245, 353, 253 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 479, 224, 483, 232 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 379, 172, 388, 180 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 468, 151, 477, 159 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 365, 140, 369, 148 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 485, 140, 494, 148 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 98, 104, 176, 111 Flags: Print Annotation: Subtype: Link Rect: 321, 722, 369, 730 Flags: Print Annotation: Subtype: Link Rect: 246, 667, 425, 675 Flags: Print Annotation: Subtype: Link Rect: 37, 87, 185, 94 Flags: Print Annotation: Subtype: Link Rect: 297, 749, 300, 757 Flags: Print Annotation: Subtype: Link Rect: 235, 748, 359, 760 Annotation: Subtype: Link Rect: 499, 616, 528, 645 Annotation: Subtype: Link Rect: 425, 59, 557, 70 Annotation: Subtype: Link Rect: 37, 49, 72, 61 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 274, 615, 283, 623 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 93, 584, 102, 592 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 231, 584, 240, 592 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 274, 553, 283, 561 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 172, 364, 181, 372 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 142, 333, 151, 341 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 391, 584, 411, 592 Flags: Print Destination: 1 Annotation: Subtype: Link Rect: 400, 458, 421, 466 Flags: Print Destination: 18 Annotation: Subtype: Link Rect: 473, 417, 494, 425 Flags: Print Destination: 18 Page: Label: 3 Annotations: Annotation: Subtype: Link Rect: 93, 385, 112, 393 Flags: Print Destination: 1 Annotation: Subtype: Link Rect: 258, 344, 267, 352 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 266, 302, 286, 310 Flags: Print Destination: 18 Annotation: Subtype: Link Rect: 507, 385, 516, 393 Flags: Print Destination: 100 Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 99, 720, 120, 728 Flags: Print Destination: 18 Annotation: Subtype: Link Rect: 37, 668, 57, 676 Flags: Print Destination: 18 Annotation: Subtype: Link Rect: 93, 563, 102, 571 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 104, 563, 113, 571 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 225, 448, 234, 456 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 408, 532, 428, 540 Flags: Print Destination: 37 Annotation: Subtype: Link Rect: 495, 427, 515, 435 Flags: Print Destination: 52 Page: Label: 5 Page: Label: 6 Annotations: Annotation: Subtype: Link Rect: 180, 657, 189, 665 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 157, 448, 200, 456 Flags: Print Destination: 37 Annotation: Subtype: Link Rect: 230, 154, 239, 162 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 309, 720, 329, 728 Flags: Print Destination: 72 Annotation: Subtype: Link Rect: 351, 699, 372, 707 Flags: Print Destination: 72 Annotation: Subtype: Link Rect: 363, 689, 386, 696 Flags: Print Destination: 72 Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 400, 105, 409, 113 Flags: Print Destination: 100 Page: Label: 8 Annotations: Annotation: Subtype: Link Rect: 114, 385, 123, 393 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 230, 228, 239, 236 Flags: Print Destination: 100 Annotation: Subtype: Link Rect: 516, 103, 557, 111 Flags: Print Annotation: Subtype: Link Rect: 306, 92, 429, 100 Flags: Print Page: Label: 9 Annotations: Annotation: Subtype: Link Rect: 268, 712, 284, 719 Flags: Print Annotation: Subtype: Link Rect: 53, 704, 237, 711 Flags: Print Annotation: Subtype: Link Rect: 53, 696, 283, 703 Flags: Print Annotation: Subtype: Link Rect: 53, 688, 230, 695 Flags: Print Annotation: Subtype: Link Rect: 53, 681, 268, 687 Flags: Print Annotation: Subtype: Link Rect: 53, 673, 285, 679 Flags: Print Annotation: Subtype: Link Rect: 53, 665, 121, 671 Flags: Print Annotation: Subtype: Link Rect: 53, 657, 284, 663 Flags: Print Annotation: Subtype: Link Rect: 53, 649, 140, 655 Flags: Print Annotation: Subtype: Link Rect: 53, 641, 273, 647 Flags: Print Annotation: Subtype: Link Rect: 53, 633, 284, 639 Flags: Print Annotation: Subtype: Link Rect: 53, 625, 279, 631 Flags: Print Annotation: Subtype: Link Rect: 53, 617, 272, 623 Flags: Print Annotation: Subtype: Link Rect: 53, 609, 146, 615 Flags: Print Annotation: Subtype: Link Rect: 53, 601, 286, 607 Flags: Print Annotation: Subtype: Link Rect: 53, 593, 263, 599 Flags: Print Annotation: Subtype: Link Rect: 53, 585, 246, 591 Flags: Print Annotation: Subtype: Link Rect: 53, 577, 271, 583 Flags: Print Annotation: Subtype: Link Rect: 53, 569, 286, 575 Flags: Print Annotation: Subtype: Link Rect: 53, 561, 146, 567 Flags: Print Annotation: Subtype: Link Rect: 53, 553, 286, 559 Flags: Print Annotation: Subtype: Link Rect: 53, 545, 264, 551 Flags: Print Annotation: Subtype: Link Rect: 53, 537, 197, 543 Flags: Print Annotation: Subtype: Link Rect: 54, 529, 286, 535 Flags: Print Annotation: Subtype: Link Rect: 54, 521, 272, 527 Flags: Print Annotation: Subtype: Link Rect: 54, 513, 286, 520 Flags: Print Annotation: Subtype: Link Rect: 54, 505, 165, 512 Flags: Print Annotation: Subtype: Link Rect: 54, 497, 286, 504 Flags: Print Annotation: Subtype: Link Rect: 54, 489, 280, 496 Flags: Print Annotation: Subtype: Link Rect: 54, 481, 177, 488 Flags: Print Annotation: Subtype: Link Rect: 54, 473, 264, 480 Flags: Print Annotation: Subtype: Link Rect: 54, 465, 195, 472 Flags: Print Annotation: Subtype: Link Rect: 54, 457, 286, 464 Flags: Print Annotation: Subtype: Link Rect: 54, 449, 283, 456 Flags: Print Annotation: Subtype: Link Rect: 54, 441, 205, 448 Flags: Print Annotation: Subtype: Link Rect: 54, 433, 286, 440 Flags: Print Annotation: Subtype: Link Rect: 54, 425, 286, 432 Flags: Print Annotation: Subtype: Link Rect: 54, 417, 214, 424 Flags: Print Annotation: Subtype: Link Rect: 323, 732, 542, 738 Flags: Print Annotation: Subtype: Link Rect: 323, 724, 514, 730 Flags: Print Annotation: Subtype: Link Rect: 323, 716, 555, 722 Flags: Print Annotation: Subtype: Link Rect: 323, 708, 415, 714 Flags: Print Annotation: Subtype: Link Rect: 323, 700, 555, 706 Flags: Print Annotation: Subtype: Link Rect: 323, 692, 514, 698 Flags: Print Annotation: Subtype: Link Rect: 323, 684, 555, 690 Flags: Print Annotation: Subtype: Link Rect: 323, 676, 388, 682 Flags: Print Annotation: Subtype: Link Rect: 323, 668, 538, 674 Flags: Print Annotation: Subtype: Link Rect: 323, 660, 540, 666 Flags: Print Annotation: Subtype: Link Rect: 323, 652, 486, 658 Flags: Print Annotation: Subtype: Link Rect: 323, 644, 546, 651 Flags: Print Annotation: Subtype: Link Rect: 323, 636, 544, 643 Flags: Print Annotation: Subtype: Link Rect: 323, 628, 348, 635 Flags: Print Annotation: Subtype: Link Rect: 323, 620, 555, 627 Flags: Print Annotation: Subtype: Link Rect: 323, 612, 351, 619 Flags: Print Annotation: Subtype: Link Rect: 323, 604, 552, 611 Flags: Print Annotation: Subtype: Link Rect: 323, 596, 427, 603 Flags: Print Annotation: Subtype: Link Rect: 323, 588, 520, 595 Flags: Print Annotation: Subtype: Link Rect: 323, 580, 555, 587 Flags: Print Annotation: Subtype: Link Rect: 323, 572, 385, 579 Flags: Print Annotation: Subtype: Link Rect: 323, 565, 545, 571 Flags: Print Annotation: Subtype: Link Rect: 323, 557, 552, 563 Flags: Print Annotation: Subtype: Link Rect: 323, 549, 390, 555 Flags: Print Annotation: Subtype: Link Rect: 323, 541, 555, 547 Flags: Print Annotation: Subtype: Link Rect: 323, 533, 429, 539 Flags: Print Annotation: Subtype: Link Rect: 323, 525, 544, 531 Flags: Print Annotation: Subtype: Link Rect: 323, 517, 481, 523 Flags: Print Annotation: Subtype: Link Rect: 323, 509, 544, 515 Flags: Print Annotation: Subtype: Link Rect: 323, 501, 546, 507 Flags: Print Annotation: Subtype: Link Rect: 323, 493, 376, 499 Flags: Print Annotation: Subtype: Link Rect: 323, 485, 555, 491 Flags: Print Annotation: Subtype: Link Rect: 323, 477, 543, 483 Flags: Print Annotation: Subtype: Link Rect: 323, 469, 552, 475 Flags: Print Annotation: Subtype: Link Rect: 323, 461, 367, 467 Flags: Print Annotation: Subtype: Link Rect: 323, 453, 543, 459 Flags: Print Annotation: Subtype: Link Rect: 323, 445, 548, 451 Flags: Print Annotation: Subtype: Link Rect: 323, 437, 372, 443 Flags: Print Annotation: Subtype: Link Rect: 436, 429, 505, 435 Flags: Print Annotation: Subtype: Link Rect: 323, 421, 545, 427 Flags: Print Annotation: Subtype: Link Rect: 323, 413, 471, 419 Flags: Print Checksum: ebc23cb3 Type: CRC32 Checksum: 2a8f730ec5b5bb1f72bf2275fb756125 Type: MD5 Checksum: 32ab85a2a5351c8445b6be78e14aa1bd49693d56 Type: SHA-1 Checksum: ae53ee15cba56d8ad3ec5dbb2674ccf911e115bcabbe1277c07684a1cf329075 Type: SHA-256