f6a5daccbb4e3250ce1d65cead3f9424 pmc_9809533.pdf 7f5c95be71f47d583606d20169cdb3f935a7a869 pmc_9809533.pdf bee1fa931e77b58241a917dd46cd4db61168e8df77a5f6ef4a842c207f181cfb pmc_9809533.pdf Title: Dynamic release of neuronal extracellular vesicles containing miR‐21a‐5p is induced by hypoxia Subject: Keywords: Author: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 11.0 (Windows) CreationDate: Mon Jan 2 09:40:52 2023 CET ModDate: Mon Jan 2 09:41:38 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 21 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 5734857 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AOFDKO+MinionPro-Regular Type 1C Custom yes yes yes 609 0 AOFDON+MinionPro-Bold Type 1C Custom yes yes yes 604 0 AOFEDM+MinionPro-It Type 1C Custom yes yes yes 599 0 AOFEPJ+STIXTwoText-Italic Type 1C Custom yes yes yes 263 0 AOFFHH+STIX-Regular Type 1C WinAnsi yes yes no 280 0 AOFFJH+STIXTwoMath Type 1C Custom yes yes yes 275 0 AOFFLH+STIXTwoText Type 1C Custom yes yes yes 270 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-04 02:29:41 CEST RepresentationInformation: pmc_9809533.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-19 02:11:31 CET Size: 5734857 Format: PDF Version: 1.6 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'jev212297-supitem-0001' cannot be found Offset: 5699996 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'jev212297-supitem-0003' cannot be found Offset: 5699996 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: 2 | MATERIAL AND METHODS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Children: Item: Title: 2.1 | Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Children: Item: Title: 2.1.1 | Permanent middle cerebral artery occlusion (pMCAO) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: 2.1.2 | Perfusion and tissue collection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: 2.2 | Cell culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Children: Item: Title: 2.2.1 | Primary mouse cortical neurons Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: 2.2.2 | Primary mouse microglia Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: 2.2.3 | Immortalised mouse cell lines Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: 2.3 | Hypoxia and inflammation in vitro models Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: 2.4 | Extracellular vesicle isolation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2.5 | Western blotting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 2.6 | Nanoparticle tracking analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2.7 | EVQuant Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 2.8 | Analysis of EV uptake and functions in vitro Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 2.9 | Cryo-electron microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 2.10 | RNase treatment of the EVs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: 2.11 | RNA extraction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 2.12 | Reverse transcription and qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 2.13 | Small RNA sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 2.14 | Global run-on sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 2.15 | HITS-clip sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 2.16 | MitoSOX and caspase-3/7 assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 2.17 | Proliferation and phagocytosis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 2.18 | Stroke patients Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: 2.19 | Figures and data analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: 3 | RESULTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Children: Item: Title: 3.1 | The levels of miR-21a-5p are increased in the in vitro and in vivo stroke models Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: 3.2 | Extracellular vesicles secreted by neuronal cells are taken up by several recipient cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: 3.3 | Hypoxia induces the release of neuronal EVs and EV associated miR-21a-5p before the cellular level of miR-21a-5p increases Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: 3.4 | EV preparations from dying neurons differ in abundance, size and contamination profile compared to the healthy cell isolated EVs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Item: Title: 3.5 | Most of the miR-21a-5p in the neuron conditioned medium is non-EV associated but the EV associated fraction is well protected from enzymatic degradation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@553a3d88 Item: Title: 3.6 | EVs from hypoxic neurons do not affect microglial proliferation or phagocytosis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a30d1e6 Item: Title: 3.7 | Peripheral blood levels of miR-21-5p correlate with the functional outcome in ischemic stroke patients Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5891e32e Item: Title: 4 | DISCUSSION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@cb0ed20 Item: Title: AUTHOR CONTRIBUTIONS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8e24743 Item: Title: ACKNOWLEDGEMENTS Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74a10858 Item: Title: CONFLICT OF INTEREST Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@23fe1d71 Item: Title: GEOLOCATION INFORMATION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@28ac3dc3 Children: Item: Title: DATA AVAILABILITY STATEMENT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@32eebfca Item: Title: REFERENCES Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4e718207 Item: Title: SUPPORTING INFORMATION Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1d371b2d Info: Title: Dynamic release of neuronal extracellular vesicles containing miR‐21a‐5p is induced by hypoxia Author: Subject: Keywords: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 11.0 (Windows) CreationDate: Mon Jan 02 10:10:52 CET 2023 ModDate: Mon Jan 02 10:11:38 CET 2023 ID: 0xf8bb891c820fd450237bd52ee567867d, 0xc21dbec8b84a89711df55639dafc974b Filters: 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