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Title:          Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
Subject:        Mycobacterium brumae is a rapid-growing, non-pathogenic Mycobacterium species, originally isolated from environmental and human samples in Barcelona, Spain.
Author:         Chantal Renau-Mínguez; Paula Herrero-Abadía; Paula Ruiz-Rodriguez; Vicente Sentandreu; Eduard Torrents; Álvaro Chiner-Oms; Manuela Torres-Puente; Iñaki Comas; Esther Julián; Mireia Coscolla
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     Item: 
      Title: Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype
      Children: 
       Item: 
        Title: Introduction
       Item: 
        Title: Materials and methods
        Children: 
         Item: 
          Title: Bacterial strains
         Item: 
          Title: Genomes
         Item: 
          Title: Drug resistance analysis
         Item: 
          Title: DNA extraction
         Item: 
          Title: Library construction and genome sequencing
         Item: 
          Title: Assembly and annotation of the completed genome
         Item: 
          Title: Virulence genes analysis
         Item: 
          Title: Immunogenic genes analysis
         Item: 
          Title: Cell wall biosynthesis genes analysis
         Item: 
          Title: Mapping and variant calling in illumina sequences
         Item: 
          Title: Phylogenetic reconstruction
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Genome composition of the reference strain
         Item: 
          Title: Phylogenetic position of Mycobacterium brumae in the genus
         Item: 
          Title: Drug resistance phenotypic and genotypic profile
         Item: 
          Title: Virulence related genes
         Item: 
          Title: Comparison of the immunogenic capacity
         Item: 
          Title: Cell wall composition
         Item: 
          Title: Intraspecific variability
       Item: 
        Title: Discussion
       Item: 
        Title: Data availability statement
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        Title: Author contributions
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        Title: Funding
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        Title: Conflict of interest
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        Title: Publisher’s note
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   Type: CRC32
  Checksum: 48f4c3be8e27858145701f8096659a0e
   Type: MD5
  Checksum: e93ce7acf952ed78015f0d53da809abe34996d62
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