2347f28c92cdeeea2dffeccda5d72e73 pmc_9864738.pdf eac0d2c296bb425b2cabb6afebf4b3d56033cf0c pmc_9864738.pdf 6b147f200e92998a1642924c8eca5390ffd50155a342ac02113578a962361359 pmc_9864738.pdf Title: Enzymatic Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor Subject: Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. This study aimed at evaluating the enzymatic synthesis of ascorbyl palmitate in a rotating basket reactor operated in sequential batches. Different commercial immobilized lipases were tested, and the most suitable reaction conditions were established. Among those lipases studied were Amano Lipase PS, Lipozyme® TL IM, Lipozyme® Novo 40086, Lipozyme® RM IM and Lipozyme® 435. Initially, the enzymes were screened based on previously defined synthesis conditions, showing clear differences in behavior. Lipozyme® 435 proved to be the best catalyst, reaching the highest values of initial reaction rate and yield. Therefore, it was selected for the following studies. Among the solvents assayed, 2-methyl-2-butanol and acetone showed the highest yields, but the operational stability of the catalyst was better in 2-methyl-2-butanol. The tests in a basket reactor showed great potential for large-scale application. Yields remained over 80% after four sequential batches, and the basket allowed for easy catalyst recycling. The results obtained in basket reactor are certainly a contribution to the enzymatic synthesis of ascorbyl palmitate as a competitive alternative to chemical synthesis. This may inspire future cost-effectiveness studies of the process to assess its potential as a viable alternative to be implemented. Keywords: rotating bed reactor; lipase; enzymatic synthesis; ascorbyl palmitate Author: Jessica Holtheuer, Luigi Tavernini, Claudia Bernal, Oscar Romero, Carminna Ottone and Lorena Wilson Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jan 17 12:19:39 2023 CET ModDate: Thu Jan 19 05:10:20 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1206763 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LBCJZW+VnURWPalladioL Type 1 Custom yes yes no 10 0 CTZCNN+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 HKZIFS+URWPalladioL-Roma Type 1 Custom yes yes no 20 0 VIJKDS+URWPalladioL-Ital Type 1 Custom yes yes no 24 0 PUSVWS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 42 0 YIVWYZ+CMSY10 Type 1 Builtin yes yes no 64 0 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yes no 159 0 BCFGOM+PalatinoLinotype TrueType WinAnsi yes yes no 162 0 BCFGOO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 165 0 BCFIFI+Arial CID TrueType Identity-H yes yes yes 168 0 RHFKGB+EURM10 Type 1 Builtin yes yes no 177 0 BCFGOK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 184 0 BCFGOL+PalatinoLinotype CID TrueType Identity-H yes yes yes 187 0 BCFGOM+PalatinoLinotype TrueType WinAnsi yes yes no 193 0 BCFGOO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 196 0 BCFIFI+Arial CID TrueType Identity-H yes yes yes 199 0 JHYTSG+CMR10 Type 1 Builtin yes yes no 211 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-10-19 02:23:37 CEST RepresentationInformation: pmc_9864738.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-01-26 02:11:39 CET Size: 1206763 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 374 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Biocatalyst Screening Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Comparison of Different Biocatalysts on Ascorbyl Palmitate Synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: The Effect of Solvent and Temperature on Ascorbyl Palmitate Synthesis by Lipozyme 435 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Solvent Effect on the Operational Stability of the Biocatalyst Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor in a Sequential Batch Operation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Children: Item: Title: Materials Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Hydrolysis Activity Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Synthesis Activity Assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Molecular Sieve Activation and Solvent Drying Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: HPLC Analysis of Reagents and Product Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Synthesis of Ascorbyl Palmitate with Different Commercial Immobilized Lipases Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Evaluation of Reactions Conditions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Operational Stability of Lipozyme 435 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Synthesis of Ascorbyl Palmitate in a Basket Reactor Operated in Sequential Batches Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Info: Title: Enzymatic Synthesis of Ascorbyl Palmitate in a Rotating Bed Reactor Author: Jessica Holtheuer, Luigi Tavernini, Claudia Bernal, Oscar Romero, Carminna Ottone and Lorena Wilson Subject: Ascorbyl palmitate, an ascorbic acid ester, is an important amphipathic antioxidant that has several applications in foods, pharmaceuticals, and cosmetics. The enzymatic synthesis of ascorbyl palmitate is very attractive, but few efforts have been made to address its process scale-up and implementation. This study aimed at evaluating the enzymatic synthesis of ascorbyl palmitate in a rotating basket reactor operated in sequential batches. Different commercial immobilized lipases were tested, and the most suitable reaction conditions were established. Among those lipases studied were Amano Lipase PS, Lipozyme® TL IM, Lipozyme® Novo 40086, Lipozyme® RM IM and Lipozyme® 435. Initially, the enzymes were screened based on previously defined synthesis conditions, showing clear differences in behavior. Lipozyme® 435 proved to be the best catalyst, reaching the highest values of initial reaction rate and yield. Therefore, it was selected for the following studies. Among the solvents assayed, 2-methyl-2-butanol and acetone showed the highest yields, but the operational stability of the catalyst was better in 2-methyl-2-butanol. The tests in a basket reactor showed great potential for large-scale application. Yields remained over 80% after four sequential batches, and the basket allowed for easy catalyst recycling. The results obtained in basket reactor are certainly a contribution to the enzymatic synthesis of ascorbyl palmitate as a competitive alternative to chemical synthesis. This may inspire future cost-effectiveness studies of the process to assess its potential as a viable alternative to be implemented. 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