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Title:          Fecal microbiota and inflammatory and antioxidant status of obese and lean dogs, and the effect of caloric restriction
Subject:        Introduction: Obesity is the most common nutritional disease in dogs, and is generally managed by caloric restriction.
Author:         Carla Giuditta Vecchiato; Stefania Golinelli; Carlo Pinna; Rachel Pilla; Jan S. Suchodolski; Asta Tvarijonaviciute; Camila Peres Rubio; Elisa Dorato; Costanza Delsante; Claudio Stefanelli; Elena Pagani; Federico Fracassi; Giacomo Biagi
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     Item: 
      Title: Fecal microbiota and inflammatory and antioxidant status of obese and lean dogs, and the effect of caloric restriction
      Children: 
       Item: 
        Title: 1. Introduction
       Item: 
        Title: 2. Materials and methods
        Children: 
         Item: 
          Title: 2.1. Animals and study design
         Item: 
          Title: 2.2. Sampling collection
         Item: 
          Title: 2.3. Laboratory analysis
         Item: 
          Title: 2.3.1. Fecal samples
         Item: 
          Title: 2.3.1.1. Chemical analysis
         Item: 
          Title: 2.3.1.2. Real-time quantitative PCR
         Item: 
          Title: 2.3.1.3. 16S rRNA gene sequencing
         Item: 
          Title: 2.3.2. Blood samples
         Item: 
          Title: 2.3.2.1. Antioxidant capacity
         Item: 
          Title: 2.3.2.2. Oxidant biomarkers
         Item: 
          Title: 2.4. Statistical analysis
       Item: 
        Title: 3. Results
        Children: 
         Item: 
          Title: 3.1. Phase 1—Fecal metabolites and microbiota, and serum analytes in CTRL and OB dogs
         Item: 
          Title: 3.1.1. Animals
         Item: 
          Title: 3.1.2. Fecal metabolites and microbiota
         Item: 
          Title: 3.1.3. Serum analytes
         Item: 
          Title: 3.2. Phase 2—Fecal metabolites and microbiota, and serum analytes in OB dogs
         Item: 
          Title: 3.2.1. Weight loss outcomes
         Item: 
          Title: 3.2.2. Fecal metabolites and microbiota
         Item: 
          Title: 3.2.3. Serum analytes
       Item: 
        Title: 4. Discussion
        Children: 
         Item: 
          Title: 4.1. Thyroid function and serum biochemical profile
         Item: 
          Title: 4.2. Inflammatory status and antioxidant response
         Item: 
          Title: 4.3. Fecal bacterial metabolome and microbiota
       Item: 
        Title: 5. Conclusion
       Item: 
        Title: Data availability statement
       Item: 
        Title: Ethics statement
       Item: 
        Title: Author contributions
       Item: 
        Title: Funding
       Item: 
        Title: Conflict of interest
       Item: 
        Title: Publisher’s note
     Item: 
      Title: References
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    Author: Carla Giuditta Vecchiato; Stefania Golinelli; Carlo Pinna; Rachel Pilla; Jan S. Suchodolski; Asta Tvarijonaviciute; Camila Peres Rubio; Elisa Dorato; Costanza Delsante; Claudio Stefanelli; Elena Pagani; Federico Fracassi; Giacomo Biagi
    Subject: Introduction: Obesity is the most common nutritional disease in dogs, and is generally managed by caloric restriction.
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      Annotation: 
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       Rect: 308, 362, 349, 352
      Annotation: 
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      Annotation: 
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       Rect: 418, 305, 495, 296
      Annotation: 
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       Rect: 448, 277, 528, 267
      Annotation: 
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       Rect: 434, 248, 482, 239
      Annotation: 
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       Rect: 308, 220, 386, 210
      Annotation: 
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       Rect: 361, 191, 430, 182
      Annotation: 
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       Rect: 413, 163, 472, 153
      Annotation: 
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       Rect: 308, 118, 378, 109
     Thumb: true
  Checksum: e56aae28
   Type: CRC32
  Checksum: 38588fad9556c89d1c8ca735a781534f
   Type: MD5
  Checksum: 9776e53913227e3e9365fa32d0ff34fa86dda21b
   Type: SHA-1