ae5311dc9f69160ceb035fda65384cf0 pmc_9911463.pdf cf464788dfa9702cefa60cbaa5c7775874a4f4bd pmc_9911463.pdf 5f2f506ad98d290792b7944d95e81757af0dabf7d08d50fb4014db5a5848c4b7 pmc_9911463.pdf Title: Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method Subject: African swine fever virus (ASFV) currently represents the biggest threat to the porcine industry worldwide, with high economic impact and severe animal health and welfare concerns Keywords: African swine fever, surveillance, disease control, LAMP PCR, isothermal amplification, point of care testing Author: Jose Alejandro Bohorquez; Saraswathi Lanka; Rosa Rosell; Marta Pérez-Simó; Mònica Alberch; Fernando Rodriguez; Llilianne Ganges; Carol W. Maddox Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Tue Jan 24 03:02:50 2023 CET ModDate: Tue Jan 24 03:02:50 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.389 x 841.663 pts Page rot: 0 File size: 13004475 bytes Optimized: yes PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DKNHGK+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 606 0 DKNHGL+AdvTTd3a5f740+fb Type 1C Custom yes yes yes 607 0 DKNHGM+AdvPSA18C Type 1C WinAnsi yes yes no 608 0 DKNHGN+AdvTTd3a5f740+20 Type 1C WinAnsi yes yes no 609 0 DKNHGO+AdvTT9b12cd41 Type 1C WinAnsi yes yes no 610 0 DKNHGP+AdvTT9b12cd41+20 Type 1C WinAnsi yes yes no 611 0 DKNHHA+AdvPSA323 Type 1C WinAnsi yes yes no 612 0 DKNHIA+AdvTT9b12cd41+fb Type 1C Custom yes yes yes 622 0 DKNHIB+AdvTT2c8ce45a Type 1C WinAnsi yes yes no 623 0 DKNHIC+AdvTT2c8ce45a+fb Type 1C Custom yes yes yes 624 0 DKNKIB+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 632 0 DKNKMB+AdvOT1ef757c0 Type 1C WinAnsi yes yes no 315 0 DKNKMC+AdvOT1ef757c0+fb Type 1C Custom yes yes yes 326 0 DKNKNC+AdvTT58d178b9+03 Type 1C Custom yes yes yes 316 0 DKNKND+AdvOT1ef757c0+22 Type 1C Custom yes yes yes 324 0 DKNKNE+AdvOT1ef757c0+20 Type 1C Custom yes yes yes 325 0 DKNLAE+AdvOTd877c31c Type 1C WinAnsi yes yes no 346 0 DKNLAF+AdvOT7d6df7ab.I Type 1C WinAnsi yes yes no 337 0 DKNLAG+AdvOTd877c31c+21 Type 1C WinAnsi yes yes no 340 0 DKNLBH+AdvOT3f84ef53 Type 1C WinAnsi yes yes no 341 0 DKNOCF+AdvOT46dcae81 Type 1C WinAnsi yes yes no 349 0 DKNOCG+AdvOT45bf69de.BI Type 1C WinAnsi yes yes no 348 0 DKNOCH+AdvOTb65e897d.B Type 1C WinAnsi yes yes no 347 0 DKOMLO+HelveticaNeueLTStd-Bd Type 1C WinAnsi yes yes no 350 0 DLEBMF+AdvOT1ef757c0+01 Type 1C MacRoman yes yes no 353 0 DLEBMG+AdvOT7d6df7ab.I+fb Type 1C Custom yes yes yes 356 0 DLEBMH+AdvOT7d6df7ab.I+20 Type 1C WinAnsi yes yes no 357 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-03-08 02:41:54 CET RepresentationInformation: pmc_9911463.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-03 02:16:05 CET Size: 13004475 Format: PDF Version: 1.4 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - 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referenced object 'B47' cannot be found Offset: 12984022 MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 704 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Children: Item: Title: 1 Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: 2 Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Children: Item: Title: 2.1 Cells and viruses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: 2.2 ASFV sequence analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: 2.3 Plasmid insert designs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: 2.4 ASFV-LAMP primer design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: 2.5 qPCR reference methods for ASFV detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: 2.6 Fluorometric LAMP reactions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: 2.7 Colorimetric LAMP reactions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: 2.8 Thermal stability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: 2.9 LAMP analytical limit of detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: 2.10 LAMP biological limit of detection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Item: Title: 2.11 LAMP testing of DNA-spiked samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: 2.12 Detection of ASFV DNA from experimentally infected animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: 2.13 ASFV-LAMP detection of viral DNA extracted using minimal equipment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: 2.14 Specificity testing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: 2.15 Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: 3 Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Children: Item: Title: 3.1 Selection of ASFV-LAMP primer candidates Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: 3.2 The ASFV-LAMP-BG2 and ASFV-LAMP-BG3 primer sets showed thermal stability Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: 3.3 The two selected ASFV-LAMP assays are able to detect as few as 1 gene copy/µL Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: 3.4 The ASFV-LAMP-BG3 assay detected target DNA in a variety of matrices Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: 3.5 The ASFV-LAMP assays showed highly efficient detection of ASFV DNA from multiple viral genotypes Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: 3.6 The ASFV-LAMP-BG3 primer set was highly efficient at detecting viral DNA from ASFV-infected tissues and clinical samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: 3.7 ASFV-LAMP amplification can be efficiently performed using minimal equipment DNA extraction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Item: Title: 4 Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7cbd213e Item: Title: Data availability statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@192d3247 Item: Title: Ethics statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3ecd23d9 Item: Title: Author contributions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@569cfc36 Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@43bd930a Item: Title: Supplementary material Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@33723e30 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@64f6106c Info: Title: Efficient detection of African Swine Fever Virus using minimal equipment through a LAMP PCR method Author: Jose Alejandro Bohorquez; Saraswathi Lanka; Rosa Rosell; Marta Pérez-Simó; Mònica Alberch; Fernando Rodriguez; Llilianne Ganges; Carol W. Maddox Subject: African swine fever virus (ASFV) currently represents the biggest threat to the porcine industry worldwide, with high economic impact and severe animal health and welfare concerns Keywords: African swine fever, surveillance, disease control, LAMP PCR, isothermal amplification, point of care testing Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Tue Jan 24 03:02:50 CET 2023 ModDate: Tue Jan 24 03:02:50 CET 2023 ID: 0xbf05a4bcdd755d5d8a6987ef28de6bd8, 0x931f91cbcca48b4293528d0b09771aee Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2919 ImageHeight: 577 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2021 ImageHeight: 668 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/png 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