d0ca71f690d0ae3982738dcbda3a71b5 pmc_9928156.pdf 53508f0d6a3c3f9bf8e52722b01b4aff3c60a8cf pmc_9928156.pdf 4740b34661ee75449374b0d05e80d3640d9003624cabf0507a0bda1579f1411e pmc_9928156.pdf Title: Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection Subject: Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows Keywords: "CTL, NKT, NK, PRRSV-1, transplacental infection" Author: Yanli Li; Ivan Díaz; Gerard Martín-Valls; Niklas Beyersdorf; Enric Mateu Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Mon Jan 30 02:33:06 2023 CET ModDate: Mon Jan 30 09:09:09 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.389 x 841.663 pts Page rot: 0 File size: 4084244 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object 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edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'B41' cannot be found Offset: 4044534 ID: PDF-HUL-122 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'B42' cannot be found Offset: 4044534 MIMEtype: application/pdf PDFMetadata: Objects: 661 FreeObjects: 1 IncrementalUpdates: 4 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Children: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Materials and methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Children: Item: Title: Farm and sampling of gilts Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Isolation of peripheral blood mononuclear cells (PBMC) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: Determination of vertical transmission: sows delivering PRRSV-1-infected or PRRSV-1-free piglets Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Item: Title: Isolation and propagation of the virus Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612679d6 Item: Title: Flow cytometry analysis of CTL NKT NK phenotype Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@11758f2a Item: Title: Cell Trace Violet proliferation assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@e720b71 Item: Title: IFN-γ and TNF-α intracellular cytokine staining assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1b26f7b2 Item: Title: IgG ELISPOT assay Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@491cc5c9 Item: Title: Statistical analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@74ad1f1f Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6a1aab78 Children: Item: Title: A higher frequency of virus-specific IgG-SC was detected in sows delivering healthy piglets in ex vivo IgG ELISPOT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@462d5aee Item: Title: Sows that did not suffer transplacental infection harbored a higher frequency of degranulated IFN-γ- and TNF-α-producing 吀 挀攀氀氀猀 琀栀愀琀 愀挀挀甀洀甀氀愀琀攀搀 椀渀 䌀䐀㐀 吀 挀攀氀氀猀 愀渀搀 䌀䐀㐀⼀䌀䐀㠃넀 䐀一 吀 挀攀氀氀猀 爀攀猀瀀攀挀琀椀瘀攀氀礩㸾൥湤潢樍ㄠ〠潢樼㰯䍲潰䉯硛〠〠㔹㔮㌸㤠㠴ㄮ㘶㍝⽁湮潴獛㈠〠删㌠〠删㐠〠删㔠〠删㘠〠删㜠〠删㠠〠删㤠〠删㄰‰⁒‱ㄠ〠删ㄲ‰⁒‱㌠〠删ㄴ‰⁒‱㔠〠删ㄶ‰⁒‱㜠〠删ㄸ‰⁒‱㤠〠剝⽐慲敮琠㌷㜠〠刯䉛㔶〠〠删㔵㤠〠删㔵㠠〠删㔵㜠〠删㔵㘠〠删㔵㔠〠剝⽃潮瑥湴猠㈱‰⁒⽒潴慴攠〯䵥摩慂潸嬰‰‵㤵⸳㠹‸㐱⸶㘳崯剥獯畲捥猠㈰‰⁒⽔祰支偡来㸾൥湤潢樍㈠〠潢樼㰯剥捴嬸ㄮ㘹㐠㔱㈮㈷㜠㠶⸰〳‵㈱⸲㤱崯卵扴祰支䱩湫⽂潲摥牛〠〠そ⽄敳琨B1 Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@69b0fd6f Item: Title: Infection status of newborn piglets correlated with the frequency of IFN-γ-producing T cells in sows Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@757942a1 Item: Title: CD4 SP and CD4/CD8α DN T cells proliferated to a higher extent in sows that delivered healthy piglets Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4a87761d Item: Title: A higher frequency of virus-specific IgG-SC was detected in sows delivering healthy piglets in ex vivo IgG ELISPOT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@66d1af89 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@8646db9 Item: Title: Data availability statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@37374a5e Item: Title: Ethics statement Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4671e53b Item: Title: Author contributions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@2db7a79b Item: Title: Funding Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6950e31 Item: Title: Acknowledgments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@b7dd107 Item: Title: Supplementary material Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@42eca56e Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@52f759d7 Info: Title: Systemic CD4 cytotoxic T cells improve protection against PRRSV-1 transplacental infection Author: Yanli Li; Ivan Díaz; Gerard Martín-Valls; Niklas Beyersdorf; Enric Mateu Subject: Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows Keywords: "CTL, NKT, NK, PRRSV-1, transplacental infection" Creator: Arbortext Advanced Print Publisher 10.0.1306/W Unicode Producer: Acrobat Distiller 7.0 (Windows) CreationDate: Mon Jan 30 02:33:06 CET 2023 ModDate: Mon Jan 30 09:09:09 CET 2023 ID: 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Mateu Background: Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the major swine pathogens causing reproductive failure in sows CTL, NKT, NK, PRRSV-1, transplacental infection uuid:600e7d46-ed9a-4b28-812d-4907d3889574 uuid:53beb965-703e-42e8-aa0a-a490906ff803 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 222, 686, 521, 707 Annotation: Subtype: Link Rect: 222, 661, 484, 682 Annotation: Subtype: Link Rect: 222, 636, 534, 657 Annotation: Subtype: Link Rect: 56, 39, 136, 46 Annotation: Subtype: Link Rect: 492, 39, 538, 46 Annotation: Subtype: Link Rect: 56, 691, 133, 706 Annotation: Subtype: Link Rect: 67, 511, 136, 518 Annotation: Subtype: Link Rect: 67, 491, 133, 498 Annotation: Subtype: Link Rect: 56, 327, 166, 334 Annotation: Subtype: Link Rect: 68, 277, 162, 284 Annotation: Subtype: Link Rect: 56, 268, 106, 275 Annotation: Subtype: Link Rect: 447, 793, 503, 800 Annotation: Subtype: Link Rect: 463, 784, 515, 791 Annotation: Subtype: Link 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