ddced29d493308801a8fb6b2dc4b0aae pmc_9960669.pdf 55b70df7ee0d9bb1b7acb96dbb8d5210f52e6026 pmc_9960669.pdf 72773ab12a5d83b13acc0ed9f84a240514158b5e760a8d4d8a293504d2fad583 pmc_9960669.pdf Title: Surface Modified -Ti-18Mo-6Nb-5Ta (wt%) Alloy for Bone Implant Applications: Composite Characterization and Cytocompatibility Assessment Subject: Commercially available titanium alloys such as Ti-6Al-4V are established in clinical use as load-bearing bone implant materials. However, concerns about the toxic effects of vanadium and aluminum have prompted the development of Al- and V-free -Ti alloys. Herein, a new alloy composed of non-toxic elements, namely Ti-18Mo-6Nb-5Ta (wt%), has been fabricated by arc melting. The resulting single -phase alloy shows improved mechanical properties (Young’s modulus and hardness) and similar corrosion behavior in simulated body fluid when compared with commercial Ti-6Al-4V. To increase the cell proliferation capability of the new biomaterial, the surface of Ti-18Mo-6Nb-5Ta was modified by electrodepositing calcium phosphate (CaP) ceramic layers. Coatings with a Ca/P ratio of 1.47 were obtained at pulse current densities, -jc, of 1.8–8.2 mA/cm2, followed by 48 h of NaOH post-treatment. The thickness of the coatings has been measured by scanning electron microscopy from an ion beam cut, resulting in an average thickness of about 5 m. Finally, cytocompatibility and cell adhesion have been evaluated using the osteosarcoma cell line Saos-2, demonstrating good biocompatibility and enhanced cell proliferation on the CaP-modified Ti-18Mo-6Nb-5Ta material compared with the bare alloy, even outperforming their CaP-modified Ti-6-Al-4V counterparts. Keywords: titanium alloy; electrodeposition; ceramic coating; indentation; corrosion resistance; cell proliferation Author: Michael Escobar, Oriol Careta, Nora Fernández Navas, Aleksandra Bartkowska, Ludovico Andrea Alberta, Jordina Fornell, Pau Solsona, Thomas Gemming, Annett Gebert, Elena Ibáñez, Andreu Blanquer, Carme Nogués, Jordi Sort and Eva Pellicer Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Feb 9 10:20:31 2023 CET ModDate: Thu Feb 9 10:24:12 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4264576 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ZYYJXI+EURB10 Type 1 Builtin yes yes no 10 0 GIFXFT+VnURWPalladioL-Bold Type 1 Custom yes yes no 14 0 ZKGJWL+VnURWPalladioL Type 1 Custom yes yes no 19 0 IUFFFR+EURM10 Type 1 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RXWYHQ+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 390 0 CQLMFU+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 403 0 DXYSTS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 411 0 LBBOBC+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 415 0 RXWYHQ+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 419 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2023-12-15 02:34:47 CET RepresentationInformation: pmc_9960669.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-02 02:17:30 CET Size: 4264576 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 621 FreeObjects: 3 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Materials Preparation Destination: subsection.2.1 Item: Title: Characterization of Structural, Physical, and Physicochemical Properties Destination: subsection.2.2 Item: Title: Cytocompatibility Studies Destination: subsection.2.3 Children: Item: Title: Cell Culture Destination: subsubsection.2.3.1 Item: Title: Cell Proliferation and Cell Viability Analysis Destination: subsubsection.2.3.2 Item: Title: Cell Morphology and Cell Adhesion Analysis Destination: subsubsection.2.3.3 Item: Title: Statistical Analysis Destination: subsubsection.2.3.4 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Fabrication and Characterization of the Ti-18Mo-6Nb-5Ta Alloy Destination: subsection.3.1 Item: Title: Electrodeposition of CaP Coatings and Their Characterization Destination: subsection.3.2 Item: Title: Cytocompatibility of CaP-Coated and Uncoated Alloys Destination: subsection.3.3 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Surface Modified -Ti-18Mo-6Nb-5Ta (wt%) Alloy for Bone Implant Applications: Composite Characterization and Cytocompatibility Assessment Author: Michael Escobar, Oriol Careta, Nora Fernández Navas, Aleksandra Bartkowska, Ludovico Andrea Alberta, Jordina Fornell, Pau Solsona, Thomas Gemming, Annett Gebert, Elena Ibáñez, Andreu Blanquer, Carme Nogués, Jordi Sort and Eva Pellicer Subject: Commercially available titanium alloys such as Ti-6Al-4V are established in clinical use as load-bearing bone implant materials. However, concerns about the toxic effects of vanadium and aluminum have prompted the development of Al- and V-free -Ti alloys. Herein, a new alloy composed of non-toxic elements, namely Ti-18Mo-6Nb-5Ta (wt%), has been fabricated by arc melting. The resulting single -phase alloy shows improved mechanical properties (Young’s modulus and hardness) and similar corrosion behavior in simulated body fluid when compared with commercial Ti-6Al-4V. To increase the cell proliferation capability of the new biomaterial, the surface of Ti-18Mo-6Nb-5Ta was modified by electrodepositing calcium phosphate (CaP) ceramic layers. Coatings with a Ca/P ratio of 1.47 were obtained at pulse current densities, -jc, of 1.8 8.2 mA/cm2, followed by 48 h of NaOH post-treatment. The thickness of the coatings has been measured by scanning electron microscopy from an ion beam cut, resulting in an average thickness of about 5 m. Finally, cytocompatibility and cell adhesion have been evaluated using the osteosarcoma cell line Saos-2, demonstrating good biocompatibility and enhanced cell proliferation on the CaP-modified Ti-18Mo-6Nb-5Ta material compared with the bare alloy, even outperforming their CaP-modified Ti-6-Al-4V counterparts. 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