73b9994017c1d092089b67e41087d5d2 pmc_9965300.pdf 70772ef9ca24ced51c186c4a2aed034fc445ba31 pmc_9965300.pdf 7dc1d30e5ae7d0638a726980a67edd27622e3a321762c998d55bcc6297a2323c pmc_9965300.pdf Title: Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats Subject: Supplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria diet either alone or combined with oral administration of the polyphenol oleuropein in obese adult male rats, compared with a control group fed standard chow and a group fed cafeteria diet. Animals were sacrificed at the age of 26 weeks and several tissues of interest were removed. The results showed that both dietary interventions reduced the adiposity index (p < 0.05 and p < 0.01, respectively), and specifically the abdominal fat depots (mesenteric: p < 0.01 and p < 0.01, respectively; and epididymal: both diets p < 0.001) and restored the decreased soleus skeletal muscle mass. Both interventions decreased leptin mRNA expression in mesenteric white adipose tissue (p < 0.05) and normalized hypothalamic Agrp mRNA expression compared to cafeteria-fed obese rats (p < 0.05). However, only the calorie-restricted cafeteria diet supplemented with oleuropein induced additional lower retroperitoneal adipose accretion (p < 0.05) and increased hypothalamic leptin receptor mRNA levels (p < 0.05). Experiments with female animals, at different doses and longer intervention periods, are needed to better determine the potential benefits of this dietary treatment. Keywords: adiponectin; calorie restriction; dietary supplements; hypothalamus; leptin; metabolic syndrome; white adipose tissue Author: Alex Subias-Gusils, Adam Álvarez-Monell, Noemi Boqué, Antoni Caimari, Roger Mariné-Casadó, Rosa M. Escorihuela and Montserrat Solanas Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Jan 18 09:39:48 2023 CET ModDate: Wed Jan 18 09:44:53 2023 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1444453 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IBIITV+URWPalladioL-Bold Type 1 Custom yes yes no 10 0 DKDBUA+URWPalladioL-Roma Type 1 Custom yes yes no 15 0 CARFRU+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 VACJGA+VnURWPalladioL Type 1 Custom yes yes no 23 0 MIDETX+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 ZZPUEX+ArialMT Type 1C WinAnsi yes yes no 47 0 TWGUDY+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 50 0 CAMXTK+EURM10 Type 1 Builtin yes yes no 72 0 TRXZTX+CMSY10 Type 1 Builtin yes yes no 79 0 JBCRLF+PazoMath Type 1 Builtin yes yes no 87 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 95 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 99 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 103 0 ArialMT TrueType WinAnsi no no no 108 0 Arial-BoldMT TrueType WinAnsi no no no 111 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 128 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 132 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 136 0 ArialMT TrueType WinAnsi no no no 141 0 Arial-BoldMT TrueType WinAnsi no no no 144 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 161 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 165 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 169 0 ArialMT TrueType WinAnsi no no no 174 0 Arial-BoldMT TrueType WinAnsi no no no 177 0 Arial-BoldItalicMT TrueType WinAnsi no no no 180 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 187 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 191 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 195 0 ArialMT TrueType WinAnsi no no no 201 0 Arial-BoldMT TrueType WinAnsi no no no 204 0 Arial-BoldItalicMT TrueType WinAnsi no no no 207 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-07-05 02:31:55 CEST RepresentationInformation: pmc_9965300.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-02 02:16:54 CET Size: 1444453 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 445 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Experimental Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: mRNA Expression Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Statistical Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Adipose Depots and Other Tissues Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: mRNA Expression in HPT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: mRNA Expression in mWAT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Info: Title: Effects of a Calorie-Restricted Cafeteria Diet and Oleuropein Supplementation on Adiposity and mRNA Expression of Energy Balance Related Genes in Obese Male Rats Author: Alex Subias-Gusils, Adam Álvarez-Monell, Noemi Boqué, Antoni Caimari, Roger Mariné-Casadó, Rosa M. Escorihuela and Montserrat Solanas Subject: Supplementation with natural bioactive compounds has been proposed to be a complementary tool to the calorie-restricted diets and physical exercise programs used to tackle human overweight, obesity and Metabolic syndrome. Herein, we evaluated the effects of 14 weeks of calorie-restricted cafeteria diet either alone or combined with oral administration of the polyphenol oleuropein in obese adult male rats, compared with a control group fed standard chow and a group fed cafeteria diet. Animals were sacrificed at the age of 26 weeks and several tissues of interest were removed. The results showed that both dietary interventions reduced the adiposity index (p < 0.05 and p < 0.01, respectively), and specifically the abdominal fat depots (mesenteric: p < 0.01 and p < 0.01, respectively; and epididymal: both diets p < 0.001) and restored the decreased soleus skeletal muscle mass. Both interventions decreased leptin mRNA expression in mesenteric white adipose tissue (p < 0.05) and normalized hypothalamic Agrp mRNA expression compared to cafeteria-fed obese rats (p < 0.05). However, only the calorie-restricted cafeteria diet supplemented with oleuropein induced additional lower retroperitoneal adipose accretion (p < 0.05) and increased hypothalamic leptin receptor mRNA levels (p < 0.05). Experiments with female animals, at different doses and longer intervention periods, are needed to better determine the potential benefits of this dietary treatment. 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