0ebb3161da3f2d99d12472fe08bba62c bioba_a2024_v9_p531.pdf ec334770fd2da6ee9bae525950f0446d09bc36e9 bioba_a2024_v9_p531.pdf d5b8ae81c01aec22340cb3ee94c7348963291173097375b47fe509b6be83ec4c bioba_a2024_v9_p531.pdf Title: Mussel-Inspired Multifunctional Polyethylene Glycol Nanoparticle Interfaces Subject: Nanoparticles (NPs) are receiving increasing interest in biomedical applications. However, due to their large surface area, in physiological environments, they tend to interact with plasma proteins, inducing their agglomeration and ultimately resulting in a substantial efficiency decrease in diagnostic and therapeutic applications. To overcome such problems, NPs are typically coated with a layer of hydrophilic and biocompatible polymers, such as PEG chains. However, few examples exist in which this property could be systematically fine-tuned and combined with added properties, such as emission. Herein, we report a novel mussel-inspired catechol-based strategy to obtain biocompatible and multifunctional coatings, using a previously developed polymerization methodology based on the formation of disulfide bridges under mild oxidative conditions. Two families of NPs were selected as the proof of concept: mesoporous silica NPs (MSNPs), due to their stability and known applications, and magnetite NPs (Fe3O4 NPs), due to their small size (<10 nm) and magnetic properties. The PEG coating confers biocompatibility on the NPs and can be further functionalized with bioactive molecules, such as glucose units, through the end carboxylic acid moieties. Once we demonstrated the feasibility of our approach to obtaining PEG-based coatings on different families of NPs, we also obtained multifunctional coatings by incorporating fluorescein functionalities. The resulting coatings not only confer biocompatibility and excellent cell internalization, but also allow for the imaging and tracking of NPs within cells. Keywords: catechol; functional coatings; bioinspired; functional nanoparticles; biocompatible; magnetite NPs; MSNPs Author: Carolina Casagualda, Alba López-Moral, Paula Alfonso-Triguero, Julia Lorenzo, Ramon Alibés, Félix Busqué and Daniel Ruiz-Molina Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Wed Sep 4 09:00:36 2024 CEST ModDate: Wed Sep 4 09:08:53 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5210316 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 ZXBFQV+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 GCQHJW+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 BNFWWD+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 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GBINBF+TimesNewRoman CID TrueType Identity-H yes yes yes 636 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-11-21 02:49:12 CET RepresentationInformation: bioba_a2024_v9_p531.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-10-25 07:07:40 CEST Size: 5210316 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 828 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: General Procedures Destination: subsection.2.1 Item: Title: Synthesis of Monomers Destination: subsection.2.2 Item: Title: Synthesis and Characterization of Oligomeric Materials P1, P2, P5, and C2 3 Destination: subsection.2.3 Item: Title: Coating of Mesoporous Silica Nanoparticles Destination: subsection.2.4 Item: Title: Coating of Magnetite Nanoparticles Destination: subsection.2.5 Children: Item: Title: Preparation of Magnetite NPs (MNPs) B48-biomimetics-3072959 Destination: subsubsection.2.5.1 Item: Title: Coating of MNPs: Synthesis of Fe3O4@P5 NPs Destination: subsubsection.2.5.2 Item: Title: Functionalization of Fe3O4@P5 NPs: Synthesis of Targeted Fe3O4@P5-Glucose NPs Destination: subsubsection.2.5.3 Item: Title: Cytotoxicity and Internalization Assays of C2 3 Coated MSNPs Destination: subsection.2.6 Children: Item: Title: Cytotoxicity Assay Destination: subsubsection.2.6.1 Item: Title: Internalization Assay Destination: subsubsection.2.6.2 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Synthesis of Monomers 1 6 and the Corresponding Oligomeric Materials P1 5 Destination: subsection.3.1 Item: Title: Coating of Mesoporous Silica Nanoparticles Destination: subsection.3.2 Item: Title: Coating of Fe3O4 NPs Destination: subsection.3.3 Item: Title: Multifunctional Coatings Destination: subsection.3.4 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: appendix.A. Info: Title: Mussel-Inspired Multifunctional Polyethylene Glycol Nanoparticle Interfaces Author: Carolina Casagualda, Alba López-Moral, Paula Alfonso-Triguero, Julia Lorenzo, Ramon Alibés, Félix Busqué and Daniel Ruiz-Molina Subject: Nanoparticles (NPs) are receiving increasing interest in biomedical applications. However, due to their large surface area, in physiological environments, they tend to interact with plasma proteins, inducing their agglomeration and ultimately resulting in a substantial efficiency decrease in diagnostic and therapeutic applications. To overcome such problems, NPs are typically coated with a layer of hydrophilic and biocompatible polymers, such as PEG chains. However, few examples exist in which this property could be systematically fine-tuned and combined with added properties, such as emission. Herein, we report a novel mussel-inspired catechol-based strategy to obtain biocompatible and multifunctional coatings, using a previously developed polymerization methodology based on the formation of disulfide bridges under mild oxidative conditions. Two families of NPs were selected as the proof of concept: mesoporous silica NPs (MSNPs), due to their stability and known applications, and magnetite NPs (Fe3O4 NPs), due to their small size (<10 nm) and magnetic properties. The PEG coating confers biocompatibility on the NPs and can be further functionalized with bioactive molecules, such as glucose units, through the end carboxylic acid moieties. Once we demonstrated the feasibility of our approach to obtaining PEG-based coatings on different families of NPs, we also obtained multifunctional coatings by incorporating fluorescein functionalities. The resulting coatings not only confer biocompatibility and excellent cell internalization, but also allow for the imaging and tracking of NPs within cells. 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