390a349d38940905641a9ab02eb81939 ANI_a2024v14p3366.pdf f2d76dfea04f8c538f791b5a948edc7996e8c99f ANI_a2024v14p3366.pdf c09cbcb5bf2b50ee6b369822288a48fd573ecc26bc35490ae0035a5de278c171 ANI_a2024v14p3366.pdf Title: Use of Different Synbiotic Strategies to Improve Gut Health in Dogs Subject: This study evaluated the effects of two synbiotic strategies on the intestinal microbiota and immune response in Beagle dogs. Twelve dogs were subjected to a crossover design with three diets: a control diet (CON), a diet supplemented with fiber and B. velezensis DSM 15544 (SYN), and the SYN diet with added porcine plasma (SYN+). Over three periods of seven weeks, fecal samples were analyzed for digestibility, short-chain fatty acids (SCFA), fecal markers, and microbiome composition, while blood samples were assessed for biochemical parameters, leucocytic counts including CD4/CD8 lymphocyte populations, and phagocytic activity. Both SYN and SYN+ diets increased the fecal volume without affecting the consistency and slightly reduced the organic matter and energy digestibility, while increasing SCFA concentrations and reducing branched-chain fatty acids. A microbiome analysis revealed no changes in the alpha diversity, but significant shifts in the beta diversity, with increases in beneficial taxa such as Faecalibacterium prausnitzii and reductions in potentially harmful bacteria like Prevotella copri. Immune response indicators showed increased fecal IgA and higher blood leukocyte counts, particularly lymphocytes and neutrophils, in the SYN diet group. Overall, both synbiotic strategies positively modulated the microbiota and immune response, though the addition of porcine plasma did not confer additional benefits. Keywords: synbiotic; dietary fiber; prebiotic; probiotic; dried plasma; immunity; dog; microbiota Author: Miquel Montserrat-Malagarriga, Lorena Castillejos, Anna Salas-Mani, Celina Torre and Susana María Martín-Orúe Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Nov 22 14:39:55 2024 CET ModDate: Fri Nov 22 14:42:23 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2256206 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LPYGPS+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 HELLCU+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 QDIYHE+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 JDARIY+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 SXQPWE+VnURWPalladioL Type 1 Custom yes yes yes 32 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Results Destination: section.3 Children: Item: Title: Fecal Quality Destination: subsection.3.1 Item: Title: Apparent Digestibility of Nutrients and Metabolizable Energy Destination: subsection.3.2 Item: Title: Fermentative Products in the Feces Destination: subsection.3.3 Item: Title: Fecal Microbiota Destination: subsection.3.4 Item: Title: Serum Biochemistry and Immune Parameters Destination: subsection.3.5 Item: Title: Discussion Destination: section.4 Children: Item: Title: Effects Driven by the Synbiotic Combination Destination: subsection.4.1 Item: Title: Specific Effects Associated with SDP Supplementation Destination: subsection.4.2 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Use of Different Synbiotic Strategies to Improve Gut Health in Dogs Author: Miquel Montserrat-Malagarriga, Lorena Castillejos, Anna Salas-Mani, Celina Torre and Susana María Martín-Orúe Subject: This study evaluated the effects of two synbiotic strategies on the intestinal microbiota and immune response in Beagle dogs. Twelve dogs were subjected to a crossover design with three diets: a control diet (CON), a diet supplemented with fiber and B. velezensis DSM 15544 (SYN), and the SYN diet with added porcine plasma (SYN+). Over three periods of seven weeks, fecal samples were analyzed for digestibility, short-chain fatty acids (SCFA), fecal markers, and microbiome composition, while blood samples were assessed for biochemical parameters, leucocytic counts including CD4/CD8 lymphocyte populations, and phagocytic activity. Both SYN and SYN+ diets increased the fecal volume without affecting the consistency and slightly reduced the organic matter and energy digestibility, while increasing SCFA concentrations and reducing branched-chain fatty acids. A microbiome analysis revealed no changes in the alpha diversity, but significant shifts in the beta diversity, with increases in beneficial taxa such as Faecalibacterium prausnitzii and reductions in potentially harmful bacteria like Prevotella copri. Immune response indicators showed increased fecal IgA and higher blood leukocyte counts, particularly lymphocytes and neutrophils, in the SYN diet group. Overall, both synbiotic strategies positively modulated the microbiota and immune response, though the addition of porcine plasma did not confer additional benefits. 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