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Hazardous effects on Drosophila Subject: Environmental Pollution, 341 (2024) 122968. doi:10.1016/j.envpol.2023.122968 Keywords: True-to-life nanoplastics,Internalization pathway,Oxidative stress,Gene expression genotoxicity,Drosophila melanogaster Author: Mohamed Alaraby Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Jan 1 09:58:09 2024 CET ModDate: Mon Jan 1 10:42:30 2024 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 5291390 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- GBNAGF+Univers Type 1C Builtin yes yes yes 738 0 IRFOQO+TeX_CM_Roman Type 1C Builtin yes yes yes 742 0 QZWOQU+CharisSIL CID TrueType Identity-H yes yes yes 746 0 ZLUPBC+CharisSIL-Bold CID TrueType Identity-H yes yes yes 751 0 IMNDIC+CharisSIL-Italic CID TrueType Identity-H yes yes yes 756 0 TSNKIJ+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 761 0 QTSNEO+STIX-Regular Type 1C Custom yes yes yes 765 0 FJNHOU+CharisSIL CID TrueType Identity-H yes yes yes 729 0 FHPTDP+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 327 0 PVJALS+CharisSIL-Italic CID TrueType Identity-H yes yes yes 320 0 AFTKZP+STIXGeneral Type 1C Custom yes yes yes 30 0 NTMBZO+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 333 0 JNRFSW+Arial-BoldMT TrueType WinAnsi yes yes no 159 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-02-10 02:32:02 CET RepresentationInformation: envpol_a2024m1v341p122968.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-02-09 17:50:26 CET Size: 5291390 Format: PDF Version: 1.7 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-149 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-122 Offset: 5248049 MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 827 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Titanium-doped PET nanoplastics, from opaque milk bottle degradation, as a model of environmental true-to-life nanoplastics ... Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 PET(Ti)NPLs obtention Destination: mk:H2_1 Item: Title: 2.2 PET(Ti)NPLs characterization Destination: mk:H2_2 Item: Title: 2.3 Drosophila melanogaster (experimental design) Destination: mk:H2_3 Children: Item: Title: 2.3.1 Egg-to-adult viability Destination: mk:H3_4 Item: Title: 2.3.2 Internalization analysis Destination: mk:H3_5 Item: Title: 2.3.3 Internalized titanium (Ti) quantification Destination: mk:H3_6 Item: Title: 2.3.4 Gene expression analysis by real-time PCR Destination: mk:H3_7 Item: Title: 2.3.5 Oxidative stress determination Destination: mk:H3_8 Item: Title: 2.3.6 Genotoxicity experiments (the comet assay) Destination: mk:H3_9 Item: Title: 2.4 Statistical analysis Destination: mk:H2_10 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 Characteristics of PET(Ti)NPLs and TiO2NPs Destination: mk:H2_11 Item: Title: 3.2 Toxicity and internalization of PET(Ti)NPLs and TiO2NPs Destination: mk:H2_12 Item: Title: 3.3 Quantification of Ti content in exposed larvae and in the resulting adults Destination: mk:H2_13 Item: Title: 3.4 Molecular responses to PET(Ti)NPLs and TiO2NPs exposures Destination: mk:H2_14 Item: Title: 3.5 Oxidative stress induction by PET(Ti)NPLs and TiO2NPs exposures Destination: mk:H2_15 Item: Title: 3.6 DNA damage induction by PET(Ti)NPLs and TiO2NPs exposures Destination: mk:H2_16 Item: Title: 4 Conclusions Destination: mk:H1_4 Item: Title: Author contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Data availability Destination: datalink3 Item: Title: Acknowledgments Destination: mk:HH1_4 Item: Title: Appendix A Supplementary data Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: Titanium-doped PET nanoplastics, from opaque milk bottle degradation, as a model of environmental true-to-life nanoplastics. Hazardous effects on Drosophila Author: Mohamed Alaraby Subject: Environmental Pollution, 341 (2024) 122968. doi:10.1016/j.envpol.2023.122968 Keywords: True-to-life nanoplastics,Internalization pathway,Oxidative stress,Gene expression genotoxicity,Drosophila melanogaster Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Mon Jan 01 09:58:09 CET 2024 ModDate: Mon Jan 01 10:42:30 CET 2024 ID: 0xf61f00b45edb4a88f6ab84b422257b0b, 0x2c8426c83e9a8b81b846abff487c20c6 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: IMNDIC+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: FHPTDP+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: QZWOQU+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: ZLUPBC+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: GBNAGF+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: GBNAGF+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: IRFOQO+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: IRFOQO+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: TSNKIJ+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: TSNKIJ+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: QTSNEO+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 8 FontDescriptor: FontName: QTSNEO+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: NTMBZO+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: NTMBZO+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: AFTKZP+STIXGeneral FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: AFTKZP+STIXGeneral Flags: Nonsymbolic FontBBox: -178, -443, 2000, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: ZLUPBC+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ZLUPBC+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: IMNDIC+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: IMNDIC+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: FHPTDP+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: FHPTDP+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: QZWOQU+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: QZWOQU+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true XMP: application/pdf 10.1016/j.envpol.2023.122968 Elsevier Ltd Environmental Pollution, 341 (2024) 122968. doi:10.1016/j.envpol.2023.122968 True-to-life nanoplastics Internalization pathway Oxidative stress Gene expression genotoxicity Drosophila melanogaster Titanium-doped PET nanoplastics, from opaque milk bottle degradation, as a model of environmental true-to-life nanoplastics. Hazardous effects on Drosophila Mohamed Alaraby Aliro Villacorta Doaa Abass Alba Hernández Ricard Marcos journal Environmental Pollution © 2023 The Authors. Published by Elsevier Ltd. 0269-7491 341 15 January 2024 2024-01-15 122968 122968 10.1016/j.envpol.2023.122968 https://doi.org/10.1016/j.envpol.2023.122968 2010-04-23 true 10.1016/j.envpol.2023.122968 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by/4.0/ VoR 10.1016/j.envpol.2023.122968 noindex 2010-04-23 true 1st January 2024 sciencedirect.com elsevier.com Elsevier 2024-01-01T09:42:30 2024-01-01T08:58:09 2024-01-01T09:42:30 True 1st January 2024 Acrobat Distiller 8.1.0 (Windows) True-to-life nanoplastics,Internalization pathway,Oxidative stress,Gene expression genotoxicity,Drosophila melanogaster uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 126, 559, 132, 569 Flags: Print Destination: 714 Annotation: Subtype: Link Rect: 133, 554, 218, 565 Flags: Print Destination: 714 Annotation: Subtype: Link Rect: 218, 559, 224, 569 Flags: Print Destination: 714 Annotation: 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