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Title:          CHIR99021 causes inactivation of Tyrosine Hydroxylase and depletion of dopamine in rat brain striatum
Subject:        Neuropharmacology, 242 (2024) 109759. doi:10.1016/j.neuropharm.2023.109759
Keywords:       Dopamine,Tyrosine Hydroxylase,Striatum,GSK3,HPLC,CHIR99021
Author:         Sally Hamdon
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      Title: CHIR99021 causes inactivation of Tyrosine Hydroxylase and depletion of dopamine in rat brain striatum
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Animals
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          Title: 2.2 Preparation of striatal minces
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          Title: 2.3 Determination of endogenous l-DOPA, DA and DOPAC levels by HPLC-EC
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          Title: 2.4 Newly-synthesized [3H]-DA determination by HPLC-UV
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          Title: 2.5 Tyrosine Hydroxylase activity in homogenates obtained from striatum
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          Title: 2.6 Western blot
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          Title: 2.7 Statistical Analysis
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        Title: 3 Results
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          Title: 3.1 CHIR99021 strongly decreases endogenous dopamine accumulation and TH activity in rat striatal minces
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          Title: 3.2 SB216763 and lithium ion do not decrease dopamine content or alter DA metabolism
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          Title: 3.3 CHIR99021 and SB216763, but not lithium, inhibit GSK3β activity on β-catenin in striatum minces
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          Title: 3.4 CHIR99021 and SB216763 decrease TH phosphorylation in serine 19, but not in serines 31 and 40
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          Title: 3.5 CHIR99021 impairs the accumulation of exogenous DA into striatal minces
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          Title: 3.6 Tetrabenazine strongly decreases endogenous dopamine accumulation
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        Title: 4 Discussion
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        Title: CRediT authorship contribution statement
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        Title: Declaration of competing interest
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        Title: Data availability
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        Title: Acknowledgements
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        Title: Appendix A Supplementary data
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        Title: References
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    Author: Sally Hamdon
    Subject: Neuropharmacology, 242 (2024) 109759. doi:10.1016/j.neuropharm.2023.109759
    Keywords: Dopamine,Tyrosine Hydroxylase,Striatum,GSK3,HPLC,CHIR99021
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  Checksum: e554b544
   Type: CRC32
  Checksum: 5c9f5c3fbad3f5c3e99fda2cefdd660d
   Type: MD5
  Checksum: 55c118d845460463dff7d8cc6c51542a5f3cfd55
   Type: SHA-1
  Checksum: 78f0a83f700d9ca206e9e565336261e9f77f42c4e4a2aab15fd605f29bc46c1f
   Type: SHA-256