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Carmona Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Fri Nov 10 19:15:22 2023 CET ModDate: Sat Nov 11 01:37:16 2023 CET Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 9971178 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AHUPVM+CharisSIL CID TrueType Identity-H yes yes yes 1191 0 ZGEUEP+STIX-Regular Type 1C Custom yes yes yes 1196 0 HNIRNJ+STIX-Italic Type 1C Custom yes yes yes 1201 0 NNLAUQ+TeX_CM_Roman Type 1C Builtin yes yes yes 1206 0 TPXCDQ+CharisSIL-Bold CID TrueType Identity-H yes yes yes 1210 0 MFLPPM+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 1215 0 ACPSBG+CharisSIL-Italic CID TrueType Identity-H yes yes yes 1219 0 GBNAGF+Univers Type 1C Builtin yes yes yes 1224 0 DZSTTR+CharisSIL CID TrueType Identity-H yes yes yes 1182 0 VVCCGG+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 532 0 QAZKBX+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 7 0 YBCBJM+CharisSIL-Italic CID TrueType Identity-H yes yes yes 525 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-03-07 02:35:34 CET RepresentationInformation: 289752.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-03-06 09:13:30 CET Size: 9971178 Format: PDF Version: 1.7 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-149 ErrorMessage: edu.harvard.hul.ois.jhove.module.pdf.PdfInvalidException: Invalid indirect destination - referenced object 'appsec1' cannot be found ID: PDF-HUL-122 Offset: 9903192 MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 1294 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Single-cell transcriptomic profiling reveals a pathogenic role of cytotoxic CD4+ T cells in giant cell arteritis Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Material and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Study samples Destination: mk:H2_1 Item: Title: 2.2 Single-cell library preparation and sequencing Destination: mk:H2_2 Item: Title: 2.3 Single-cell data analysis Destination: mk:H2_3 Item: Title: 2.4 Flow cytometric analysis Destination: mk:H2_4 Item: Title: 2.5 Single-cell TCR analysis Destination: mk:H2_5 Item: Title: 2.6 Pseudotime analysis Destination: mk:H2_6 Item: Title: 2.7 Transcription factor analysis Destination: mk:H2_7 Item: Title: 2.8 Single-cell resolution drug targeting Destination: mk:H2_8 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 Single-cell landscape of CD4+ T cells in GCA Destination: mk:H2_9 Item: Title: 3.2 CD4+ CTLs are expanded in active GCA patients compared to those in remission Destination: mk:H2_10 Item: Title: 3.3 Effector Tregs are decreased in GCA patients Destination: mk:H2_11 Item: Title: 3.4 CD4+ T cells from active patients present higher expression levels of cytotoxicity-related genes and decreased regulato ... Destination: mk:H2_12 Item: Title: 3.5 CTLs show a marked clonal expansion Destination: mk:H2_13 Item: Title: 3.6 CD4+ T cells of GCA patients in remission shifted toward less differentiated states Destination: mk:H2_14 Item: Title: 3.7 Differential activity of relevant transcription factors in GCA patients Destination: mk:H2_15 Item: Title: 3.8 Potentially repositionable drugs in GCA Destination: mk:H2_16 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: 5 Conclusions Destination: mk:H1_5 Item: Title: Contributors Destination: mk:H1_6 Item: Title: Funding Destination: mk:H1_7 Item: Title: Ethics approval Destination: mk:H1_8 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Data availability Destination: datalink3 Item: Title: Acknowledgements Destination: mk:HH1_4 Item: Title: Appendix A Supplementary data Destination: mk:H1_9 Item: Title: References Destination: mk:HH2_1 Info: Title: Single-cell transcriptomic profiling reveals a pathogenic role of cytotoxic CD4+ T cells in giant cell arteritis Author: Elio G. Carmona Subject: Journal of Autoimmunity, 142 (2024) 103124. doi:10.1016/j.jaut.2023.103124 Keywords: Giant cell arteritis,Single-cell RNA sequencing,CD4+ T cells,T cell receptor profiling Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Fri Nov 10 19:15:22 CET 2023 ModDate: Sat Nov 11 01:37:16 CET 2023 ID: 0xed76a5132258016b5c0bd81b43da6160, 0xeb7b81ebe82c7b886a65caa62467e2a2 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: ACPSBG+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: AHUPVM+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: QAZKBX+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: TPXCDQ+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: HNIRNJ+STIX-Italic FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: HNIRNJ+STIX-Italic Flags: Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: VVCCGG+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: VVCCGG+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: NNLAUQ+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: NNLAUQ+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: GBNAGF+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: GBNAGF+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: ZGEUEP+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 12 FontDescriptor: FontName: ZGEUEP+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MFLPPM+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: MFLPPM+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true CIDFontType2: Font: BaseFont: ACPSBG+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ACPSBG+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: AHUPVM+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: AHUPVM+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: QAZKBX+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: QAZKBX+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: TPXCDQ+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: TPXCDQ+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true XMP: application/pdf 10.1016/j.jaut.2023.103124 Elsevier Ltd Journal of Autoimmunity, 142 (2024) 103124. doi:10.1016/j.jaut.2023.103124 Giant cell arteritis Single-cell RNA sequencing CD4+ T cells T cell receptor profiling Single-cell transcriptomic profiling reveals a pathogenic role of cytotoxic CD4+ T cells in giant cell arteritis Elio G. Carmona José Luis Callejas-Rubio Enrique Raya Raquel Ríos-Fernández Gonzalo Villanueva-Martín María C. Cid José Hernández-Rodríguez Esteban Ballestar Bernd Timmermann Norberto Ortego-Centeno Javier Martín Ana Márquez journal Journal of Autoimmunity © 2023 The Authors. 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