61b7877f4961728a0a2caf8bac8c0f65 SciTotEnv_a2024v934p173236.pdf 9b2aeae13943fcc02bbaa3ecf49bc4345f546190 SciTotEnv_a2024v934p173236.pdf 333854b3c0bd56e182f900a3feddc9ce3e714751737c4b39e9b637a6252ecc6b SciTotEnv_a2024v934p173236.pdf Title: Hazard assessment of nanoplastics is driven by their surface-functionalization. Effects in human-derived primary endothelial cells Subject: Science of the Total Environment, 934 (2024) 173236. doi:10.1016/j.scitotenv.2024.173236 Keywords: HUVEC,Polystyrene nanoplastics,Functionalization,Internalization,Genotoxicity Author: Joan Martín-Pérez Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue May 28 17:53:18 2024 CEST ModDate: Tue May 28 18:13:36 2024 CEST Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 4745592 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QMMDIX+CharisSIL CID TrueType Identity-H yes yes yes 838 0 ORVAOY+STIX-Regular Type 1C Custom yes yes yes 843 0 GBNAGF+Univers Type 1C Builtin yes yes yes 848 0 XUQIXG+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 852 0 IPUFCG+TeX_CM_Roman Type 1C Builtin yes yes yes 856 0 IKEQYJ+CharisSIL-Italic CID TrueType Identity-H yes yes yes 860 0 UXBLCW+CharisSIL CID TrueType Identity-H yes yes yes 829 0 PSWCWA+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 365 0 OPGJIL+CharisSIL-Bold CID TrueType Identity-H yes yes yes 370 0 EBDXZY+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 375 0 HAQIZO+CharisSIL-Italic CID TrueType Identity-H yes yes yes 358 0 PNFGLD+STIX-Italic Type 1C Custom yes yes yes 40 0 ZJYGCW+TeX_CM_Maths_Extension Type 1C Builtin yes yes yes 45 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-07-11 02:46:20 CEST RepresentationInformation: SciTotEnv_a2024v934p173236.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-07-10 19:10:03 CEST Size: 4745592 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 929 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Hazard assessment of nanoplastics is driven by their surface-functionalization. Effects in human-derived primary endothelia ... Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Polystyrene nanoplastics characterization Destination: mk:H2_1 Item: Title: 2.2 Cell line and culture conditions Destination: mk:H2_2 Item: Title: 2.3 Polystyrene nanoplastics treatments Destination: mk:H2_3 Item: Title: 2.4 Cell viability assay Destination: mk:H2_4 Item: Title: 2.5 Cellular uptake (FACS, fluorescent activated cell sorter) and cell complexity (flow cytometry) Destination: mk:H2_5 Item: Title: 2.6 Cellular uptake assessment by confocal microscopy Destination: mk:H2_6 Item: Title: 2.7 Cellular uptake assessment by transmission electron microscopy (TEM) Destination: mk:H2_7 Item: Title: 2.8 Production of intracellular reactive oxygen species (ROS) Destination: mk:H2_8 Item: Title: 2.9 Genotoxicity induction detected by the comet assay Destination: mk:H2_9 Item: Title: 2.10 Statistical analysis Destination: mk:H2_10 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 Polystyrene nanoplastics characterization Destination: mk:H2_11 Item: Title: 3.2 Toxicity of the evaluated PS-NPLs Destination: mk:H2_12 Item: Title: 3.3 Assessing cell internalization of PSNPLs by different analytical methods Destination: mk:H2_13 Children: Item: Title: 3.3.1 Cellular internalization by FACS Destination: mk:H3_14 Item: Title: 3.3.2 Cellular internalization by confocal microscopy Destination: mk:H3_15 Item: Title: 3.3.3 Cellular internalization by TEM Destination: mk:H3_16 Item: Title: 3.4 Size and inner complexity as assessed by flow cytometry Destination: mk:H2_17 Item: Title: 3.5 Intracellular ROS production Destination: mk:H2_18 Item: Title: 3.6 Genotoxicity detection by the comet assay Destination: mk:H2_19 Item: Title: 4 Conclusions Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_4 Item: Title: Data availability Destination: datalink5 Item: Title: Acknowledgments Destination: mk:HH1_6 Item: Title: Appendix A Supplementary data Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: Hazard assessment of nanoplastics is driven by their surface-functionalization. Effects in human-derived primary endothelial cells Author: Joan Martín-Pérez Subject: Science of the Total Environment, 934 (2024) 173236. doi:10.1016/j.scitotenv.2024.173236 Keywords: HUVEC,Polystyrene nanoplastics,Functionalization,Internalization,Genotoxicity Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue May 28 17:53:18 CEST 2024 ModDate: Tue May 28 18:13:36 CEST 2024 ID: 0x0f7092bb38b4ceb5b81ebcadf460b690, 0x9a4dc5fd4187c4cb9f570de56fc49724 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: OPGJIL+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: QMMDIX+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: IKEQYJ+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: PSWCWA+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: GBNAGF+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: GBNAGF+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: XUQIXG+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: XUQIXG+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: EBDXZY+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: EBDXZY+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: IPUFCG+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: IPUFCG+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: PNFGLD+STIX-Italic FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: PNFGLD+STIX-Italic Flags: Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: ORVAOY+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 9 FontDescriptor: FontName: ORVAOY+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: ZJYGCW+TeX_CM_Maths_Extension FontSubset: true FirstChar: 1 LastChar: 150 FontDescriptor: FontName: ZJYGCW+TeX_CM_Maths_Extension Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true CIDFontType2: Font: BaseFont: OPGJIL+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: OPGJIL+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: QMMDIX+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: QMMDIX+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: IKEQYJ+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: IKEQYJ+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: PSWCWA+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: PSWCWA+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true XMP: application/pdf 10.1016/j.scitotenv.2024.173236 Elsevier B.V. Science of the Total Environment, 934 (2024) 173236. doi:10.1016/j.scitotenv.2024.173236 HUVEC Polystyrene nanoplastics Functionalization Internalization Genotoxicity Hazard assessment of nanoplastics is driven by their surface-functionalization. Effects in human-derived primary endothelial cells Joan Martín-Pérez Aliro Villacorta Gooya Banaei Michelle Morataya-Reyes Alireza Tavakolpournegari Ricard Marcos Alba Hernández Alba García-Rodriguez journal Science of the Total Environment © 2024 The Author(s). Published by Elsevier B.V. 0048-9697 934 15 July 2024 2024-07-15 173236 173236 10.1016/j.scitotenv.2024.173236 https://doi.org/10.1016/j.scitotenv.2024.173236 2010-04-23 true 10.1016/j.scitotenv.2024.173236 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by/4.0/ VoR 10.1016/j.scitotenv.2024.173236 noindex 2010-04-23 true 28th May 2024 sciencedirect.com elsevier.com Elsevier 2024-05-28T16:13:36 2024-05-28T15:53:18 2024-05-28T16:13:36 True 28th May 2024 Acrobat Distiller 8.1.0 (Windows) HUVEC,Polystyrene nanoplastics,Functionalization,Internalization,Genotoxicity uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 1 https://www.elsevier.com/tdm/tdmrep-policy.json Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 125, 559, 216, 569 Flags: Print Destination: 809 Annotation: Subtype: Link Rect: 217, 554, 294, 565 Flags: Print Destination: 809 Annotation: Subtype: Link Rect: 294, 554, 432, 565 Flags: Print Destination: 809 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