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Quesada-López Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Jan 16 08:27:27 2024 CET ModDate: Wed Jan 24 20:31:36 2024 CET Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 2950303 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WHZCXB+CharisSIL CID TrueType Identity-H yes yes yes 591 0 KKDBAV+CharisSIL-Bold CID TrueType Identity-H yes yes yes 596 0 VXCJFZ+Univers Type 1C Builtin yes yes yes 601 0 KCRVGR+STIX-Italic Type 1C Custom yes yes yes 605 0 ERNRUV+TeX_CM_Roman Type 1C Builtin yes yes yes 610 0 NQILRU+CharisSIL-Italic CID TrueType Identity-H yes yes yes 614 0 SEDKEL+CharisSIL CID TrueType Identity-H yes yes yes 582 0 NMPOOG+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 235 0 YKIJSX+STIX-Regular Type 1C Custom yes yes yes 239 0 BPRWCF+CharisSIL-Italic CID TrueType Identity-H yes yes yes 227 0 DHMSPY+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 244 0 LXLVMZ+STIXTwoMath Type 1C Custom yes yes yes 35 0 NTMBZO+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 251 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-01 08:20:09 CEST RepresentationInformation: 298680.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-07-22 12:00:53 CEST Size: 2950303 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 695 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Divergent effects of the antiretroviral drugs, dolutegravir, tenofovir alafenamide, and tenofovir disoproxil fumarate, on h ... Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Reagents Destination: mk:H2_1 Item: Title: 2.2 Cell culture Destination: mk:H2_2 Item: Title: 2.3 Cytotoxicity assessment Destination: mk:H2_3 Item: Title: 2.4 Cell proliferation assays Destination: mk:H2_4 Item: Title: 2.5 Assessment of lipid accumulation in adipocytes Destination: mk:H2_5 Item: Title: 2.6 RNA isolation, conventional reverse transcription, and quantitative polymerase chain reaction (qRT-PCR) Destination: mk:H2_6 Item: Title: 2.7 Western blot assay Destination: mk:H2_7 Item: Title: 2.8 Secretion of adipokines and cytokines by adipocytes Destination: mk:H2_8 Item: Title: 2.9 Cell respiration assays Destination: mk:H2_9 Item: Title: 2.10 Glycerol release to the culture media Destination: mk:H2_10 Item: Title: 2.11 Statistical analyses Destination: mk:H2_11 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 DTG alone or in combination with TAF and TDF do not affect lipid accumulation but rather alter mRNA expression of adipo ... Destination: mk:H2_12 Item: Title: 3.2 TAF and TDF ameliorate the DTG-induced mRNA downregulation of mitochondrial OXPHOS components and respiration in human ... Destination: mk:H2_13 Item: Title: 3.3 TAF, but not TDF, prevents DTG-induced inflammation but exacerbates cellular stress profile in differentiating human ad ... Destination: mk:H2_14 Item: Title: 3.4 TAF and TDF ameliorate DTG-induced impairment of OXPHOS subunits and basal respiration in fully differentiated human ad ... Destination: mk:H2_15 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_3 Item: Title: Data availability Destination: datalink4 Item: Title: Acknowledgments Destination: mk:HH1_5 Item: Title: References Destination: mk:HH2_1 Info: Title: Divergent effects of the antiretroviral drugs, dolutegravir, tenofovir alafenamide, and tenofovir disoproxil fumarate, on human adipocyte function Author: T. Quesada-López Subject: Biochemical Pharmacology, 220 (2024) 116010. doi:10.1016/j.bcp.2023.116010 Keywords: Adipocyte,Adiposity,Dolutegravir,Tenofovir alafenamide,Tenofovir disoproxil fumarate,Adipokine Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Tue Jan 16 08:27:27 CET 2024 ModDate: Wed Jan 24 20:31:36 CET 2024 ID: 0xae711e04c26b3fb51f3713581d660ed5, 0x3e05c9a54cf37496a2009992f3ce89a8 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: KKDBAV+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: DHMSPY+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: NQILRU+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: WHZCXB+CharisSIL Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: ERNRUV+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: ERNRUV+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: LXLVMZ+STIXTwoMath FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: LXLVMZ+STIXTwoMath Flags: Nonsymbolic FontBBox: -994, -1641, 3072, 2627 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: VXCJFZ+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: VXCJFZ+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: NMPOOG+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: NMPOOG+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: NTMBZO+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: NTMBZO+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: KCRVGR+STIX-Italic FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: KCRVGR+STIX-Italic Flags: Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: YKIJSX+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 12 FontDescriptor: FontName: YKIJSX+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: WHZCXB+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: WHZCXB+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: KKDBAV+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: KKDBAV+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: DHMSPY+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: DHMSPY+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: NQILRU+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: NQILRU+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true XMP: application/pdf 10.1016/j.bcp.2023.116010 Elsevier Inc. Biochemical Pharmacology, 220 (2024) 116010. doi:10.1016/j.bcp.2023.116010 Adipocyte Adiposity Dolutegravir Tenofovir alafenamide Tenofovir disoproxil fumarate Adipokine Divergent effects of the antiretroviral drugs, dolutegravir, tenofovir alafenamide, and tenofovir disoproxil fumarate, on human adipocyte function T. Quesada-López R. Cereijo A. Blasco-Roset A. Mestres-Arenas P. Prieto J.C. Domingo F. Villarroya P. Domingo M. Giralt journal Biochemical Pharmacology © 2023 The Author(s). Published by Elsevier Inc. 0006-2952 220 1 February 2024 2024-02-01 116010 116010 10.1016/j.bcp.2023.116010 https://doi.org/10.1016/j.bcp.2023.116010 2010-04-23 true 10.1016/j.bcp.2023.116010 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by-nc/4.0/ VoR 10.1016/j.bcp.2023.116010 noindex 2010-04-23 true 24th January 2024 sciencedirect.com elsevier.com Elsevier 2024-01-24T19:31:36 2024-01-16T07:27:27 2024-01-24T19:31:36 True 24th January 2024 Acrobat Distiller 8.1.0 (Windows) Adipocyte,Adiposity,Dolutegravir,Tenofovir alafenamide,Tenofovir disoproxil fumarate,Adipokine uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 122, 559, 128, 569 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 129, 559, 136, 569 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 137, 559, 143, 569 Flags: Print Destination: 567 Annotation: Subtype: Link Rect: 143, 559, 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