da2b76277dcb29f108c54cd1b4502f95 300117.pdf e0ea71f03c4651271f03ca8f7bc18230f0842ad5 300117.pdf b0aa73d6ec5618c981f69bc8a792f127cadb0240a93f8127fedbb49fc1e0092d 300117.pdf Title: Relationship between Respiratory Microbiome and Systemic Inflammatory Markers in COPD: A Pilot Study Subject: The respiratory microbiome may influence the development and progression of COPD by modulating local immune and inflammatory events. We aimed to investigate whether relative changes in respiratory bacterial abundance are also associated with systemic inflammation, and explore their relationship with the main clinical COPD phenotypes. Multiplex analysis of inflammatory markers and transcript eosinophil-related markers were analyzed on peripheral blood in a cohort of stable COPD patients (n = 72). Respiratory microbiome composition was analyzed by 16S rRNA microbial sequencing on spontaneous sputum. Spearman correlations were applied to test the relationship between the microbiome composition and systemic inflammation. The concentration of the plasma IL-8 showed an inverted correlation with the relative abundance of 17 bacterial genera in the whole COPD cohort. COPD patients categorized as eosinophilic showed positive relationships with blood eosinophil markers and inversely correlated with the degree of airway obstruction and the number of exacerbations during the previous year. COPD patients categorized as frequent exacerbators were enriched with the bacterial genera Pseudomonas which, in turn, was positively associated with the severity of airflow limitation and the prior year’s exacerbation history. The associative relationships of the sputum microbiome with the severity of the disease emphasize the relevance of the interaction between the respiratory microbiota and systemic inflammation. Keywords: COPD; respiratory microbiome; systemic inflammation; frequent exacerbators; eosinophils Author: Carme Casadevall, Sara Quero, Laura Millares, Rosa Faner, Borja G. Cosío, Germán Peces-Barba, Ady Castro-Acosta, Concepción Montón, Alexandre Palou, Sergi Pascual-Guardia, Alvar Agustí, Joaquim Gea, Eduard Monsó and on behalf of the BIOMEPOC group Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Mon Aug 5 03:44:44 2024 CEST ModDate: Mon Aug 5 09:08:49 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2272067 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 VDZJHA+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 OENWTF+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 FKKUHQ+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 SDIDBK+VnURWPalladioL Type 1 Custom yes yes yes 32 0 IRWHZX+TimesNewRomanPSMT Type 1C 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TrueType Identity-H yes yes yes 241 0 AGFKKG+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 247 0 AGFKKH+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 250 0 AGFKKI+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 253 0 KPHRXZ+VnURWPalladioL-Italic Type 1 Custom yes yes yes 273 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-09-03 02:37:19 CEST RepresentationInformation: 300117.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-09-02 15:50:03 CEST Size: 2272067 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 465 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Demographic and Clinical Features of Participants Destination: subsection.2.1 Item: Title: All Patients: Blood Inflammatory Mediators and Microbiome Interactions Destination: subsection.2.2 Item: Title: Blood Inflammatory Mediators and Host Microbiome Interactions in the FE Phenotype Destination: subsection.2.3 Item: Title: Blood Inflammatory Mediators and Host Microbiome Interactions in the EOS Pheno/Endotype Destination: subsection.2.4 Item: Title: Discussion Destination: section.3 Children: Item: Title: Microbiome and Inflammation in COPD Destination: subsection.3.1 Item: Title: The FE Phenotype Destination: subsection.3.2 Item: Title: The EOS Phenotype/Endotype Destination: subsection.3.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Study Design and Population Destination: subsection.4.1 Item: Title: Samples Processing Destination: subsection.4.2 Item: Title: 16S rRNA Gene Sequencing Analysis Destination: subsection.4.3 Item: Title: Measurement of Systemic Inflammatory Mediators Destination: subsection.4.4 Item: Title: RT-qPCR Assays Destination: subsection.4.5 Item: Title: Statistical Analyses Destination: subsection.4.6 Item: Title: Appendix A Destination: appendix.A. Item: Title: References Destination: appendix.B. Info: Title: Relationship between Respiratory Microbiome and Systemic Inflammatory Markers in COPD: A Pilot Study Author: Carme Casadevall, Sara Quero, Laura Millares, Rosa Faner, Borja G. Cosío, Germán Peces-Barba, Ady Castro-Acosta, Concepción Montón, Alexandre Palou, Sergi Pascual-Guardia, Alvar Agustí, Joaquim Gea, Eduard Monsó and on behalf of the BIOMEPOC group Subject: The respiratory microbiome may influence the development and progression of COPD by modulating local immune and inflammatory events. We aimed to investigate whether relative changes in respiratory bacterial abundance are also associated with systemic inflammation, and explore their relationship with the main clinical COPD phenotypes. Multiplex analysis of inflammatory markers and transcript eosinophil-related markers were analyzed on peripheral blood in a cohort of stable COPD patients (n = 72). Respiratory microbiome composition was analyzed by 16S rRNA microbial sequencing on spontaneous sputum. Spearman correlations were applied to test the relationship between the microbiome composition and systemic inflammation. The concentration of the plasma IL-8 showed an inverted correlation with the relative abundance of 17 bacterial genera in the whole COPD cohort. COPD patients categorized as eosinophilic showed positive relationships with blood eosinophil markers and inversely correlated with the degree of airway obstruction and the number of exacerbations during the previous year. COPD patients categorized as frequent exacerbators were enriched with the bacterial genera Pseudomonas which, in turn, was positively associated with the severity of airflow limitation and the prior year’s exacerbation history. The associative relationships of the sputum microbiome with the severity of the disease emphasize the relevance of the interaction between the respiratory microbiota and systemic inflammation. 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