887226403644e517b3dd83b8fc8e5acf fermentation-10-00481-v2.pdf ea78e5edfabe2b1cc6561dccfe6ac4d5b0b04591 fermentation-10-00481-v2.pdf e8cfbbbc11adab61bd92202b8da8cd20332b98256451ef98f015630e3481e32a fermentation-10-00481-v2.pdf Title: Harnessing Packed-Bed Bioreactors’ Potential in Solid-State Fermentation: The Case of Beauveria bassiana Conidia Production Subject: The packed-bed bioreactor is among the most promising reactor configurations for solid-state fermentation. However, the bed thickness poses several limitations involving mass and energy transfer, heat generation, and the homogeneity of the material, hampering its development at the industrial scale. Fungi are among the most promising microorganisms used in this configuration; however, only polypropylene bags and trays are used at the industrial scale. In this work, Beauveria bassiana is used to demonstrate the potential of solid-state fermentation for conidia production. A scale-up from 0.5 L to 22 L is presented, starting with substrate selection, optimization via design of experiments and 22 L batches. The optimized parameters were 70% moisture, 6.5 106 conidia mL-1 inoculum concentration, 20 mL min-1 airflow, 25 C; temperature, and 40 C/N ratio. After optimization, beer draff was chosen as the preferred substrate for scale-up. Air-filled porosity was found to be the key parameter in fungal solid-state fermentation scale-up, establishing values of around 80% as necessary for fungal conidia production when working in a 22 L packed-bed bioreactor. When compared with the tray bioreactor, the packed bed obtained higher conidia production due to its better use of the total reactor volume. Our study harnesses the potential of the packed-bed bioreactor and serves as a base for further scale-up to industrial scale. Keywords: solid-state fermentation; packed-bed bioreactor; Beauveria bassiana; conidia production; air-filled porosity Author: Arnau Sala, Adriana Artola, Raquel Barrena and Antoni Sánchez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Wed Sep 18 09:14:25 2024 CEST ModDate: Wed Sep 18 09:54:19 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1882599 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 DHEIVL+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 YMMXZH+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 VXTLQZ+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 PDZRND+URWPalladioL-BoldItal Type 1 Custom yes yes yes 32 0 RGUFCP+CMSY10 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241 0 NEAMEH+PalatinoLinotype TrueType WinAnsi yes yes no 247 0 NEAMFI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 250 0 NEAMMI+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 253 0 NEAMMJ+TimesNewRoman CID TrueType Identity-H yes yes yes 256 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-11-14 02:53:08 CET RepresentationInformation: fermentation-10-00481-v2.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-10-08 14:45:01 CEST Size: 1882599 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 450 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Fungal Strain and Inoculum Preparation Destination: subsection.2.1 Item: Title: Raw Materials Destination: subsection.2.2 Item: Title: Solid-State Fermentation Experimental Setups Destination: subsection.2.3 Children: Item: Title: Packed-Bed Reactors: 0.5 L Destination: subsubsection.2.3.1 Item: Title: Packed-Bed Reactors: 22 L Bioreactor Destination: subsubsection.2.3.2 Item: Title: Tray Bioreactor Destination: subsubsection.2.3.3 Item: Title: Oxygen Uptake Rate Destination: subsubsection.2.3.4 Item: Title: Conidia Counting Destination: subsection.2.4 Item: Title: Total Sugar Content Analysis Destination: subsection.2.5 Item: Title: Analytical Methods Destination: subsection.2.6 Item: Title: Statistical Analyses Destination: subsection.2.7 Item: Title: Results Destination: section.3 Children: Item: Title: Substrate Selection Destination: subsection.3.1 Item: Title: Optimization in 0.5 L Reactors Destination: subsection.3.2 Item: Title: The 22 L Experiments Destination: subsection.3.3 Item: Title: Tray Bioreactor Comparison Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.6 Info: Title: Harnessing Packed-Bed Bioreactors’ Potential in Solid-State Fermentation: The Case of Beauveria bassiana Conidia Production Author: Arnau Sala, Adriana Artola, Raquel Barrena and Antoni Sánchez Subject: The packed-bed bioreactor is among the most promising reactor configurations for solid-state fermentation. However, the bed thickness poses several limitations involving mass and energy transfer, heat generation, and the homogeneity of the material, hampering its development at the industrial scale. Fungi are among the most promising microorganisms used in this configuration; however, only polypropylene bags and trays are used at the industrial scale. In this work, Beauveria bassiana is used to demonstrate the potential of solid-state fermentation for conidia production. A scale-up from 0.5 L to 22 L is presented, starting with substrate selection, optimization via design of experiments and 22 L batches. The optimized parameters were 70% moisture, 6.5 106 conidia mL-1 inoculum concentration, 20 mL min-1 airflow, 25 C; temperature, and 40 C/N ratio. After optimization, beer draff was chosen as the preferred substrate for scale-up. Air-filled porosity was found to be the key parameter in fungal solid-state fermentation scale-up, establishing values of around 80% as necessary for fungal conidia production when working in a 22 L packed-bed bioreactor. When compared with the tray bioreactor, the packed bed obtained higher conidia production due to its better use of the total reactor volume. Our study harnesses the potential of the packed-bed bioreactor and serves as a base for further scale-up to industrial scale. 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