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Destination: mk:title Children: Item: Title: Introduction Destination: mk:H1_1 Item: Title: Materials and methods Destination: mk:H1_2 Children: Item: Title: Materials and cell lines Destination: mk:H2_1 Item: Title: Cell growth, viability, and emergence of resistant assays Destination: mk:H2_2 Item: Title: Western blot analysis Destination: mk:H2_3 Item: Title: Flow cytometry Destination: mk:H2_4 Item: Title: DNA purification and NGS sequencing analysis Destination: mk:H2_5 Item: Title: RNA extraction and NanoString® nCounter assay Destination: mk:H2_6 Item: Title: Human sera and ELISAs Destination: mk:H2_7 Item: Title: Results Destination: mk:H1_3 Children: Item: Title: Anti-EGF VacAbs suppressed the stimulatory effects of hEGF in cell lines with KRAS, NRAS, and BRAF mutations Destination: mk:H2_8 Item: Title: hEGF significantly reduced the antiproliferative effects of drugs targeting the MAPK/ERK and PI3K/Akt pathways in tumor cells Destination: mk:H2_9 Item: Title: Anti-EGF VacAbs enhanced the activity of inhibitors targeting the MAPK/ERK and PI3K/Akt pathways, outperforming cetuximab i ... Destination: mk:H2_10 Item: Title: Anti-EGF VacAbs in combination with inhibitors targeting the MAPK/ERK and PI3K/Akt pathways efficiently blocked the corresp ... Destination: mk:H2_11 Item: Title: Anti-EGF VacAbs delayed the appearance of resistant colonies to inhibitors targeting the MAPK/ERK and PI3K/Akt pathways Destination: mk:H2_12 Item: Title: Colonies resistant to encorafenib or the combinations with cetuximab and anti-EGF VacAbs showed different phospho-protein a ... Destination: mk:H2_13 Item: Title: Sera of KRAS-mut patients immunized with the anti-EGF vaccine showed lower levels of EGF and suppressed the activation in v ... Destination: mk:H2_14 Item: Title: Discussion Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Acknowledgments Destination: mk:HH1_3 Item: Title: Supplementary materials Destination: mk:H1_6 Item: Title: References Destination: mk:HH2_1 Info: Title: Vaccine antibodies against a synthetic epidermal growth factor variant enhance the antitumor effects of inhibitors targeting the MAPK/ERK and PI3K/Akt pathways Author: Silvia García-Roman Subject: Translational Oncology, 40 (2024) 101878. doi:10.1016/j.tranon.2024.101878 Keywords: EGF,Vaccine,Inhibitors,Akt,ERK Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Sat Jan 13 15:57:55 CET 2024 ModDate: Sat Jan 13 15:59:36 CET 2024 ID: 0x30746061a23f8963027633060c1973f8, 0x71124a96b4933e03e00904f079ce44c0 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: 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Flags: Print Annotation: Subtype: Link Rect: 323, 286, 536, 292 Flags: Print Annotation: Subtype: Link Rect: 323, 278, 543, 284 Flags: Print Annotation: Subtype: Link Rect: 323, 270, 436, 276 Flags: Print Annotation: Subtype: Link Rect: 323, 262, 555, 268 Flags: Print Annotation: Subtype: Link Rect: 323, 254, 552, 260 Flags: Print Annotation: Subtype: Link Rect: 323, 246, 543, 252 Flags: Print Annotation: Subtype: Link Rect: 323, 238, 420, 244 Flags: Print Annotation: Subtype: Link Rect: 323, 230, 555, 236 Flags: Print Annotation: Subtype: Link Rect: 323, 222, 553, 228 Flags: Print Annotation: Subtype: Link Rect: 323, 214, 341, 220 Flags: Print Checksum: 4eae4b07 Type: CRC32 Checksum: 5ad72629a1ff74cdac0924b6dbec3ab0 Type: MD5 Checksum: 13c87394ec566a5bb4982b5ad38771d94e5eccc6 Type: SHA-1 Checksum: 1f250805e99f6d91ea10b8f5cfa0f45223351c2c6e7b34071276f81a5e33a4f6 Type: SHA-256