d233a4fc05a57e7efe8e812895750243 302196.pdf 673a3707b2a0937013d561693f2ab6b11ed0d1cf 302196.pdf 0c18f32319330db83e0d9ad5c1c71cdaf448979adfc2208f80bd721c64740af3 302196.pdf Title: Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3a protein levels Subject: Neuroscience Letters, 830 (2024) 137770. doi:10.1016/j.neulet.2024.137770 Keywords: Reelin,MeCP2,DNMT3a,DNA methylation,Stress,Sex differences Author: Carla Liria Sánchez-Lafuente Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Thu May 2 14:59:51 2024 CEST ModDate: Thu May 2 15:29:08 2024 CEST Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 3287118 bytes Optimized: yes PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ORDPHR+CharisSIL CID TrueType Identity-H yes yes yes 474 0 XVCOVL+STIX-Regular Type 1C Custom yes yes yes 479 0 GBNAGF+Univers Type 1C Builtin yes yes yes 484 0 RUZRAP+CharisSIL-Bold CID TrueType Identity-H yes yes yes 488 0 PTCBOE+TeX_CM_Roman Type 1C Builtin yes yes yes 493 0 JMVRCA+CharisSIL-Italic CID TrueType Identity-H yes yes yes 497 0 LHEBAM+CharisSIL CID TrueType Identity-H yes yes yes 465 0 XPXBEB+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 188 0 FGEKJX+STIXGeneral Type 1C Custom yes yes yes 193 0 WZVHHM+STIX-Italic Type 1C Custom yes yes yes 7 0 QBKAFZ+TeX_CM_Maths_Symbols Type 1C Builtin yes yes yes 198 0 AHXZXA+CharisSIL-Italic CID TrueType Identity-H yes yes yes 181 0 VVCCGG+TeX_CM_Maths_Italic Type 1C Builtin yes yes yes 39 0 WVMCAJ+CharisSIL-BoldItalic CID TrueType Identity-H yes yes yes 205 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-01-11 02:45:59 CET RepresentationInformation: 302196.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-01-10 15:12:04 CET Size: 3287118 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF, Tagged PDF PDFMetadata: Objects: 568 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3 ... Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Methodology Destination: mk:H1_2 Children: Item: Title: 2.1 Experimental design Destination: mk:H2_1 Item: Title: 2.2 Sample preparation Destination: mk:H2_2 Item: Title: 2.3 Nuclear extraction Destination: mk:H2_3 Item: Title: 2.4 Electrophoresis and Western blotting Destination: mk:H2_4 Item: Title: 2.5 DNA purification Destination: mk:H2_5 Item: Title: 2.6 Bisulfite-Sequencing and PCR Destination: mk:H2_6 Item: Title: 2.7 Statistical analysis Destination: mk:H2_7 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 CORT has sexually dimorphic effects on reelin fragments, MeCP2 and DNMT3a protein levels in the hypothalamus Destination: mk:H2_8 Item: Title: 3.2 Alterations in reelin protein levels were not paralleled by changes in Reln promoter methylation Destination: mk:H2_9 Children: Item: Title: 3.2.1 Bisulfite sequencing revealed that 15 different CpG sites were unmethylated in all samples Destination: mk:H3_10 Item: Title: 3.2.2 Methylation-sensitive primers revealed that the two CpG islands close to the TSS were unmethylated in all samples Destination: mk:H3_11 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: 5 Conclusion Destination: mk:H1_5 Item: Title: CRediT authorship contribution statement Destination: mk:H1_6 Item: Title: Declaration of Competing Interest Destination: mk:HH1_3 Item: Title: Data availability Destination: datalink4 Item: Title: Acknowledgements Destination: mk:HH1_5 Item: Title: References Destination: mk:HH2_1 Info: Title: Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3a protein levels Author: Carla Liria Sánchez-Lafuente Subject: Neuroscience Letters, 830 (2024) 137770. doi:10.1016/j.neulet.2024.137770 Keywords: Reelin,MeCP2,DNMT3a,DNA methylation,Stress,Sex differences Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Thu May 02 14:59:51 CEST 2024 ModDate: Thu May 02 15:29:08 CEST 2024 ID: 0xf2e080ff1055ecf46375c7d39b6838e6, 0x0c3f69d4e6088368bb39e2cccd000dea Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 236 ImageHeight: 298 BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 248 ImageHeight: 271 BitsPerSample: 8 BitsPerSampleUnit: integer Fonts: Type0: Font: BaseFont: JMVRCA+CharisSIL-Italic Encoding: Identity-H ToUnicode: true Font: BaseFont: RUZRAP+CharisSIL-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: ORDPHR+CharisSIL Encoding: Identity-H ToUnicode: true Font: BaseFont: XPXBEB+TimesNewRomanPSMT Encoding: Identity-H ToUnicode: true Font: BaseFont: WVMCAJ+CharisSIL-BoldItalic Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: FGEKJX+STIXGeneral FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: FGEKJX+STIXGeneral Flags: Nonsymbolic FontBBox: -178, -443, 2000, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: GBNAGF+Univers FontSubset: true FirstChar: 32 LastChar: 251 FontDescriptor: FontName: GBNAGF+Univers Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: QBKAFZ+TeX_CM_Maths_Symbols FontSubset: true FirstChar: 1 LastChar: 138 FontDescriptor: FontName: QBKAFZ+TeX_CM_Maths_Symbols Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: WZVHHM+STIX-Italic FontSubset: true FirstChar: 1 LastChar: 1 FontDescriptor: FontName: WZVHHM+STIX-Italic Flags: Nonsymbolic, Italic FontBBox: -970, -305, 1429, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: VVCCGG+TeX_CM_Maths_Italic FontSubset: true FirstChar: 1 LastChar: 127 FontDescriptor: FontName: VVCCGG+TeX_CM_Maths_Italic Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: PTCBOE+TeX_CM_Roman FontSubset: true FirstChar: 1 LastChar: 128 FontDescriptor: FontName: PTCBOE+TeX_CM_Roman Flags: Symbolic FontBBox: -50, -180, 1000, 720 FontFile3: true ToUnicode: true Font: BaseFont: XVCOVL+STIX-Regular FontSubset: true FirstChar: 1 LastChar: 9 FontDescriptor: FontName: XVCOVL+STIX-Regular Flags: Nonsymbolic FontBBox: -970, -486, 2208, 1023 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true CIDFontType2: Font: BaseFont: JMVRCA+CharisSIL-Italic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: JMVRCA+CharisSIL-Italic Flags: Nonsymbolic, Italic FontBBox: -716, -533, 1627, 1318 FontFile2: true Font: BaseFont: RUZRAP+CharisSIL-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: RUZRAP+CharisSIL-Bold Flags: Nonsymbolic, ForceBold FontBBox: -720, -533, 1759, 1320 FontFile2: true Font: BaseFont: ORDPHR+CharisSIL CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: ORDPHR+CharisSIL Flags: Nonsymbolic FontBBox: -692, -533, 1623, 1319 FontFile2: true Font: BaseFont: XPXBEB+TimesNewRomanPSMT CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: XPXBEB+TimesNewRomanPSMT Flags: Nonsymbolic FontBBox: -568, -307, 2000, 1007 FontFile2: true Font: BaseFont: WVMCAJ+CharisSIL-BoldItalic CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: WVMCAJ+CharisSIL-BoldItalic Flags: Nonsymbolic, Italic, ForceBold FontBBox: -741, -533, 1795, 1320 FontFile2: true XMP: application/pdf 10.1016/j.neulet.2024.137770 Elsevier B.V. Neuroscience Letters, 830 (2024) 137770. doi:10.1016/j.neulet.2024.137770 Reelin MeCP2 DNMT3a DNA methylation Stress Sex differences Chronic corticosterone exposure in rats induces sex-specific alterations in hypothalamic reelin fragments, MeCP2, and DNMT3a protein levels Carla Liria Sánchez-Lafuente Laura Martinez-Verbo Jenessa N. Johnston Jennifer Floyd Manel Esteller Lisa E. Kalynchuk Juan Ausió Hector J. Caruncho journal Neuroscience Letters © 2024 The Authors. Published by Elsevier B.V. 0304-3940 830 1 May 2024 2024-05-01 137770 137770 10.1016/j.neulet.2024.137770 https://doi.org/10.1016/j.neulet.2024.137770 2010-04-23 true 10.1016/j.neulet.2024.137770 elsevier.com sciencedirect.com http://creativecommons.org/licenses/by-nc/4.0/ VoR 10.1016/j.neulet.2024.137770 noindex 2010-04-23 true 2nd May 2024 sciencedirect.com elsevier.com Elsevier 2024-05-02T13:29:08 2024-05-02T12:59:51 2024-05-02T13:29:08 True 2nd May 2024 Acrobat Distiller 8.1.0 (Windows) Reelin,MeCP2,DNMT3a,DNA methylation,Stress,Sex differences uuid:1b499bed-4ce8-4c0c-b682-0d58baae1cbe uuid:b6ed5266-03cb-4855-90c0-636283357f42 1 https://www.elsevier.com/tdm/tdmrep-policy.json Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 172, 571, 285, 582 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 285, 571, 391, 582 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 391, 576, 397, 586 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 398, 571, 476, 582 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 476, 571, 483, 582 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 106, 563, 113, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 113, 563, 119, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 120, 563, 127, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 128, 558, 224, 569 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 224, 558, 288, 569 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 288, 563, 293, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 294, 566, 298, 571 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 294, 563, 300, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 301, 558, 405, 569 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 405, 563, 411, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 412, 566, 416, 571 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 412, 563, 418, 574 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 419, 558, 423, 569 Flags: Print Destination: 450 Annotation: Subtype: Link Rect: 95, 222, 113, 230 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 275, 201, 286, 209 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 173, 191, 191, 199 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 537, 254, 555, 262 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 420, 212, 438, 220 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 103, 123, 151, 130 Flags: Print Annotation: Subtype: Link Rect: 189, 123, 270, 130 Flags: Print Annotation: Subtype: Link Rect: 321, 722, 369, 730 Flags: Print Annotation: Subtype: Link Rect: 275, 667, 396, 675 Flags: Print Annotation: Subtype: Link Rect: 37, 87, 186, 94 Flags: Print Annotation: Subtype: Link Rect: 297, 749, 300, 757 Flags: Print Annotation: Subtype: Link Rect: 232, 748, 362, 760 Annotation: Subtype: Link Rect: 499, 616, 528, 645 Annotation: Subtype: Link Rect: 425, 57, 557, 69 Annotation: Subtype: Link Rect: 37, 48, 62, 59 Page: Label: 2 Annotations: Annotation: Subtype: Link Rect: 244, 699, 259, 707 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 197, 689, 213, 696 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 167, 615, 182, 623 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 270, 584, 286, 592 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 222, 563, 250, 571 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 162, 553, 178, 561 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 214, 542, 230, 550 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 205, 532, 221, 540 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 229, 511, 256, 519 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 68, 479, 106, 487 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 143, 469, 158, 477 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 236, 165, 263, 173 Flags: Print Destination: 146 Page: Label: 3 Page: Label: 4 Annotations: Annotation: Subtype: Link Rect: 174, 605, 193, 613 Flags: Print Destination: 24 Annotation: Subtype: Link Rect: 227, 490, 246, 498 Flags: Print Destination: 24 Annotation: Subtype: Link Rect: 253, 448, 274, 456 Flags: Print Destination: 24 Annotation: Subtype: Link Rect: 479, 563, 499, 571 Flags: Print Destination: 43 Annotation: Subtype: Link Rect: 461, 500, 481, 508 Flags: Print Destination: 63 Annotation: Subtype: Link Rect: 450, 479, 469, 487 Flags: Print Destination: 63 Page: Label: 5 Annotations: Annotation: Subtype: Link Rect: 81, 238, 92, 246 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 191, 144, 218, 152 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 37, 112, 53, 120 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 37, 81, 53, 89 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 306, 426, 333, 434 Flags: Print Destination: 83 Annotation: Subtype: Link Rect: 521, 384, 537, 392 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 505, 248, 532, 256 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 539, 217, 555, 225 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 358, 196, 374, 204 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 306, 154, 322, 162 Flags: Print Destination: 146 Annotation: Subtype: Link Rect: 329, 123, 345, 131 Flags: Print Destination: 146 Page: Label: 6 Annotations: Annotation: Subtype: Link Rect: 344, 696, 470, 703 Flags: Print Annotation: Subtype: Link Rect: 483, 673, 555, 679 Flags: Print Annotation: Subtype: Link Rect: 322, 665, 500, 671 Flags: Print Annotation: Subtype: Link Rect: 322, 657, 418, 663 Flags: Print Annotation: Subtype: Link Rect: 510, 633, 555, 639 Flags: Print Annotation: Subtype: Link Rect: 322, 625, 411, 631 Flags: Print Annotation: Subtype: Link Rect: 437, 609, 553, 615 Flags: Print Annotation: Subtype: Link Rect: 480, 585, 555, 591 Flags: Print Annotation: Subtype: Link Rect: 322, 577, 378, 583 Flags: Print Annotation: Subtype: Link Rect: 529, 561, 551, 567 Flags: Print Annotation: Subtype: Link Rect: 322, 553, 422, 559 Flags: Print Annotation: Subtype: Link Rect: 480, 537, 555, 543 Flags: Print Annotation: Subtype: Link Rect: 322, 529, 386, 535 Flags: Print Annotation: Subtype: Link Rect: 504, 513, 549, 520 Flags: Print Annotation: Subtype: Link Rect: 322, 505, 437, 512 Flags: Print Annotation: Subtype: Link Rect: 445, 473, 522, 480 Flags: Print Annotation: Subtype: Link Rect: 322, 465, 383, 472 Flags: Print Annotation: Subtype: Link Rect: 373, 433, 514, 440 Flags: Print Annotation: Subtype: Link Rect: 464, 417, 552, 424 Flags: Print Annotation: Subtype: Link Rect: 323, 410, 347, 416 Flags: Print Annotation: Subtype: Link Rect: 359, 386, 540, 392 Flags: Print Annotation: Subtype: Link Rect: 505, 362, 550, 368 Flags: Print Annotation: Subtype: Link Rect: 323, 354, 412, 360 Flags: Print Annotation: Subtype: Link Rect: 407, 330, 529, 336 Flags: Print Annotation: Subtype: Link Rect: 510, 306, 555, 312 Flags: Print Annotation: Subtype: Link Rect: 323, 298, 396, 304 Flags: Print Annotation: Subtype: Link Rect: 489, 274, 534, 280 Flags: Print Annotation: Subtype: Link Rect: 323, 266, 399, 272 Flags: Print Annotation: Subtype: Link Rect: 533, 242, 555, 249 Flags: Print Annotation: Subtype: Link Rect: 323, 234, 423, 241 Flags: Print Annotation: Subtype: Link Rect: 351, 210, 448, 217 Flags: Print Annotation: Subtype: Link Rect: 380, 186, 495, 193 Flags: Print Annotation: Subtype: Link Rect: 355, 115, 476, 121 Flags: Print Annotation: Subtype: Link Rect: 409, 91, 544, 97 Flags: Print Annotation: Subtype: Link Rect: 427, 59, 550, 65 Flags: Print Page: Label: 7 Annotations: Annotation: Subtype: Link Rect: 174, 724, 281, 730 Flags: Print Annotation: Subtype: Link Rect: 141, 700, 264, 706 Flags: Print Annotation: Subtype: Link Rect: 82, 620, 210, 627 Flags: Print Annotation: Subtype: Link Rect: 104, 572, 248, 579 Flags: Print Annotation: Subtype: Link Rect: 533, 724, 555, 730 Flags: Print Annotation: Subtype: Link Rect: 323, 716, 414, 722 Flags: Print Annotation: Subtype: Link Rect: 403, 692, 551, 698 Flags: Print Annotation: Subtype: Link Rect: 521, 676, 554, 682 Flags: Print Annotation: Subtype: Link Rect: 323, 668, 398, 674 Flags: Print Annotation: Subtype: Link Rect: 366, 644, 490, 651 Flags: Print Annotation: Subtype: Link Rect: 533, 628, 555, 635 Flags: Print Annotation: Subtype: Link Rect: 323, 620, 421, 627 Flags: Print Annotation: Subtype: Link Rect: 510, 596, 555, 603 Flags: Print Annotation: Subtype: Link Rect: 323, 588, 390, 595 Flags: Print Annotation: Subtype: Link Rect: 323, 557, 450, 563 Flags: Print Checksum: 594ca4a8 Type: CRC32 Checksum: d233a4fc05a57e7efe8e812895750243 Type: MD5 Checksum: 673a3707b2a0937013d561693f2ab6b11ed0d1cf Type: SHA-1 Checksum: 0c18f32319330db83e0d9ad5c1c71cdaf448979adfc2208f80bd721c64740af3 Type: SHA-256