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626 FreeObjects: 3 IncrementalUpdates: 3 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Interleukin-16 is increased in obesity and alters adipogenesis and inflammation in vitro Destination: bmark_-827047116 Children: Item: Title: 1 Introduction Destination: s1 Item: Title: 2 Methods Destination: s2 Children: Item: Title: 2.1 Study participants Destination: s2_1 Item: Title: 2.2 Human serological analysis Destination: s2_2 Item: Title: 2.3 Adipose tissue collection and RNA isolation and processing Destination: s2_3 Item: Title: 2.4 Cell culture experiments Destination: s2_4 Item: Title: 2.5 Oil red O staining of 3T3-L1 preadipocytes Destination: s2_5 Item: Title: 2.6 Cell RNA extraction and quantification Destination: s2_6 Item: Title: 2.7 Statistical analysis Destination: s2_7 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 IL-16 expression was highest in vWAT from individuals with obesity and was related to inflammatory pathways and serum IL-16 levels were modulated after bariatric surgery Destination: s3_1 Item: Title: 3.2 IL-16 gene expression was related to inflammatory and immune cell activation pathways Destination: s3_2 Item: Title: 3.3 IL-16 altered adipogenesis, fibrosis, and glucose and lipid metabolism during 3T3-L1 differentiation Destination: s3_3 Item: Title: 3.4 IL-16 altered inflammation, glucose and lipid metabolism, and remodeling in mature 3T3-L1 adipocytes Destination: s3_4 Item: Title: 3.5 IL-16 treatment blunted palmitate-induced lipid accumulation, lipolysis, and altered inflammation in mature 3T3-L1 adipocytes Destination: s3_5 Item: Title: 4 Discussion Destination: s4 Item: Title: 5 Limitations Destination: s5 Item: Title: 6 Conclusion Destination: s6 Item: Title: Data availability statement Destination: sx Item: Title: Ethics statement Destination: s7 Item: Title: Author contributions Destination: s8 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Conflict of interest Destination: bmark_1089982005 Item: Title: Publisher’s note Destination: bmark_-1256977648 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Interleukin-16 is increased in obesity and alters adipogenesis and inflammation in vitro Author: Marjorie Reyes-Farias Subject: Reyes-Farias M, Fernández-García P, Corrales P, González L, Soria-Gondek A, Martínez E, Pellitero S, Tarascó J, Moreno P, Sumoy L, Medina-Gómez G, Sánchez-Infantes D and Herrero L (2024) Interleukin-16 is increased in obesity and alters adipogenesis and Keywords: obesity, immunometabolism, IL-16, inflammation, adipocytes CreationDate: Tue Mar 12 07:27:37 CET 2024 ModDate: Tue Mar 12 07:27:37 CET 2024 ID: 0x56c7b6b4f68e044b4a11e2ff04cd2f3e, 0xb52c37ce3d844442acc4418dbac8fb69 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: 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Martínez, Pellitero, Tarascó, Moreno, Sumoy, Medina-Gómez, Sánchez-Infantes and Herrero. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Interleukin-16 is increased in obesity and alters adipogenesis and inflammation in vitro obesity immunometabolism IL-16 inflammation adipocytes obesity, immunometabolism, IL-16, inflammation, adipocytes True https://www.frontiersin.org/legal/copyright-statement 2024-03-12T14:27:37+08:00 2024-03-12T14:27:37+08:00 2024-03-12T14:27:37+08:00 uuid:8803837b-e9ea-40b5-aa1c-e2165faa4f56 uuid:9867b474-8130-4a29-8a87-89a06da754e0 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 221, 685, 500, 706 Annotation: Subtype: Link Rect: 221, 660, 524, 681 Annotation: Subtype: Link Rect: 221, 635, 464, 656 Annotation: Subtype: Link Rect: 49, 33, 135, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Annotation: Subtype: Link Rect: 49, 690, 125, 705 Annotation: Subtype: Link Rect: 60, 528, 112, 535 Annotation: Subtype: Link Rect: 60, 508, 132, 515 Annotation: Subtype: Link Rect: 49, 354, 156, 361 Annotation: Subtype: Link Rect: 101, 286, 194, 293 Annotation: Subtype: Link Rect: 49, 277, 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40 Checksum: 49ab2ded Type: CRC32 Checksum: e1b06fe1dddefaf41f46a077ceb6375a Type: MD5 Checksum: 399cd23b7d9550863d0526726bdb5dd924bb1a6c Type: SHA-1 Checksum: 1311d269dc8de01525f3d9c6b17d158afc9b7e6151c540d2e881b146f7990233 Type: SHA-256