0ea8b2cb5d593ec0de375c05edba41c0 306963.pdf f0fdb0ae6bfdb7325065a6a6748bf68d65b430d3 306963.pdf ad7f3056a6511267e0d967a237034951dce8060bbcad268bd428bf3580b857c5 306963.pdf Title: The Lipidomic Profile Is Associated with the Dietary Pattern in Subjects with and without Diabetes Mellitus from a Mediterranean Area Subject: Lipid functions can be influenced by genetics, age, disease states, and lifestyle factors, particularly dietary patterns, which are crucial in diabetes management. Lipidomics is an expanding field involving the comprehensive exploration of lipids from biological samples. In this cross-sectional study, 396 participants from a Mediterranean region, including individuals with type 1 diabetes (T1D), type 2 diabetes (T2D), and non-diabetic individuals, underwent lipidomic profiling and dietary assessment. Participants completed validated food frequency questionnaires, and lipid analysis was conducted using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC/MS). Multiple linear regression models were used to determine the association between lipid features and dietary patterns. Across all subjects, acylcarnitines (AcCa) and triglycerides (TG) displayed negative associations with the alternate Healthy Eating Index (aHEI), indicating a link between lipidomic profiles and dietary habits. Various lipid species (LS) showed positive and negative associations with dietary carbohydrates, fats, and proteins. Notably, in the interaction analysis between diabetes and the aHEI, we found some lysophosphatidylcholines (LPC) that showed a similar direction with respect to aHEI in non-diabetic individuals and T2D subjects, while an opposite direction was observed in T1D subjects. The study highlights the significant association between lipidomic profiles and dietary habits in people with and without diabetes, particularly emphasizing the role of healthy dietary choices, as reflected by the aHEI, in modulating lipid concentrations. These findings underscore the importance of dietary interventions to improve metabolic health outcomes, especially in the context of diabetes management. Keywords: lipidomic; diabetes; dietary pattern; lipid species Author: Marina Idalia Rojo-López, Maria Barranco-Altirriba, Joana Rossell, Maria Antentas, Esmeralda Castelblanco, Oscar Yanes, Ralf J. M. Weber, Gavin R. Lloyd, Catherine Winder, Warwick B. Dunn, Josep Julve, Minerva Granado-Casas and Dídac Mauricio Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Sat Jun 8 10:26:52 2024 CEST ModDate: Sat Jun 8 10:28:45 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1574262 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 AHXFCK+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 EMQQZU+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 FYOBIO+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 AEPYJE+VnURWPalladioL Type 1 Custom yes yes yes 32 0 HGMNRX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 VORBJF+CMSY10 Type 1 Builtin yes yes yes 71 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 76 0 QSECBH+URWPalladioL-BoldItal Type 1 Custom yes yes yes 87 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 100 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 104 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 108 0 BCDHEE+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 112 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 119 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 126 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 145 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 149 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 153 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 157 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 164 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 181 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 185 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 189 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 193 0 BCDJEE+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 200 0 SYVAVM+VnURWPalladioL-Italic Type 1 Custom yes yes yes 221 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-07 02:47:18 CET RepresentationInformation: 306963.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-06 19:41:17 CET Size: 1574262 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 421 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Design Destination: subsection.2.1 Item: Title: Clinical Data Destination: subsection.2.2 Item: Title: Dietary Pattern Assessment Destination: subsection.2.3 Item: Title: Lipidomic Study Destination: subsection.2.4 Item: Title: Data Analysis Destination: subsection.2.5 Item: Title: Statistical Analysis Destination: subsection.2.6 Item: Title: Results Destination: section.3 Children: Item: Title: Demographic and Clinical Characteristics Destination: subsection.3.1 Item: Title: Lipids Associated with Dietary Pattern Destination: subsection.3.2 Item: Title: Lipids and Macronutrient Intake Destination: subsection.3.3 Item: Title: Interaction between Diabetes and the Dietary Pattern Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: The Lipidomic Profile Is Associated with the Dietary Pattern in Subjects with and without Diabetes Mellitus from a Mediterranean Area Author: Marina Idalia Rojo-López, Maria Barranco-Altirriba, Joana Rossell, Maria Antentas, Esmeralda Castelblanco, Oscar Yanes, Ralf J. M. Weber, Gavin R. Lloyd, Catherine Winder, Warwick B. Dunn, Josep Julve, Minerva Granado-Casas and Dídac Mauricio Subject: Lipid functions can be influenced by genetics, age, disease states, and lifestyle factors, particularly dietary patterns, which are crucial in diabetes management. Lipidomics is an expanding field involving the comprehensive exploration of lipids from biological samples. In this cross-sectional study, 396 participants from a Mediterranean region, including individuals with type 1 diabetes (T1D), type 2 diabetes (T2D), and non-diabetic individuals, underwent lipidomic profiling and dietary assessment. Participants completed validated food frequency questionnaires, and lipid analysis was conducted using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC/MS). Multiple linear regression models were used to determine the association between lipid features and dietary patterns. Across all subjects, acylcarnitines (AcCa) and triglycerides (TG) displayed negative associations with the alternate Healthy Eating Index (aHEI), indicating a link between lipidomic profiles and dietary habits. Various lipid species (LS) showed positive and negative associations with dietary carbohydrates, fats, and proteins. Notably, in the interaction analysis between diabetes and the aHEI, we found some lysophosphatidylcholines (LPC) that showed a similar direction with respect to aHEI in non-diabetic individuals and T2D subjects, while an opposite direction was observed in T1D subjects. The study highlights the significant association between lipidomic profiles and dietary habits in people with and without diabetes, particularly emphasizing the role of healthy dietary choices, as reflected by the aHEI, in modulating lipid concentrations. These findings underscore the importance of dietary interventions to improve metabolic health outcomes, especially in the context of diabetes management. 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