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Title:           Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides
Subject:         Gualdrón-López M, Ayllon-Hermida A, Cortes-Serra N, Resa-Infante P, Bech-Serra JJ, Aparici-Herraiz I, Nicolau-Fernandez M, Erkizia I, Gutierrez-Chamorro L, Marfil S, Pradenas E, Ávila Nieto C, Cucurull B, Montaner-Tarbés S, Muelas M, Sotil R, Ballana E, Urrea V, Fraile L, Montoya M, Vergara J, Segales J, Carrillo J, Izquierdo-Useros N, Blanco J, Fernandez-Becerra C, de La Torre C, Pinazo M-J, Martinez-Picado J and del Portillo HA (2024) Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides. Front. Cell. Infect. Microbiol. 14:1442743. doi: 10.3389/fcimb.2024.1442743
Keywords:        COVID-19 patients; SARS-CoV-2; antibody response; extracellular vesicles; immunocapture (CD9); ganglioside-capture (CD169/Siglec-1); size-exclusion chromatography (SEC); proteomics profiling
Author:          Melisa Gualdrón-López
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      Title: Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides
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       Item: 
        Title: Introduction
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       Item: 
        Title: Materials and methods
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          Title: Subject details and experimental model
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         Item: 
          Title: Hamster model
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         Item: 
          Title: Cell lines
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         Item: 
          Title: Sample collection and procedures
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         Item: 
          Title: SARS-CoV-2 PCR detection and viral load quantification in serum samples
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         Item: 
          Title: Determination of anti-SARS-CoV-2 antibodies by enzyme linked immunosorbent assay
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         Item: 
          Title: Pseudovirus generation and neutralization assay
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         Item: 
          Title: SARS-CoV2 hamster in vivo model
          Destination: s2_8
         Item: 
          Title: SARS-CoV-2 infection of Vero cells and lysate obtention for western blotting
          Destination: s2_9
         Item: 
          Title: CD9 Direct immune-affinity capture of EVs from plasma
          Destination: s2_10
         Item: 
          Title: mS1 capture of EVs from plasma
          Destination: s2_11
         Item: 
          Title: Size exclusion chromatography of plasma
          Destination: s2_12
         Item: 
          Title: CD9/CD63/CD81 Direct Immune-affinity capture (DIC) of EVs from plasma
          Destination: s2_13
         Item: 
          Title: Nanoparticle tracking analysis
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         Item: 
          Title: Western blotting
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         Item: 
          Title: Flow cytometry bead-based assay
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         Item: 
          Title: Protein extraction and digestion
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           Item: 
            Title: CD9+ and mS1 captured EVs
            Destination: s2_17_1
           Item: 
            Title: Human EVs isolated by SEC
            Destination: s2_17_2
           Item: 
            Title: Hamster EVs isolated by SEC
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         Item: 
          Title: Liquid chromatography mass spectrometry
          Destination: s2_18
          Children: 
           Item: 
            Title: CD9+ EVs (DIA)
            Destination: s2_18_1
           Item: 
            Title: mS1 captured EVs (DIA)
            Destination: s2_18_2
           Item: 
            Title: Human EVs isolated by SEC (DDA)
            Destination: s2_18_3
           Item: 
            Title: Hamster EVs isolated by SEC (DDA)
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         Item: 
          Title: Proteins identification and statistical analysis
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           Item: 
            Title: CD9+ EVs (DIA)
            Destination: s2_19_1
           Item: 
            Title: mS1 captured EVs (DIA)
            Destination: s2_19_2
           Item: 
            Title: Human EVs isolated by SEC
            Destination: s2_19_3
           Item: 
            Title: Hamster EVs isolated by SEC
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         Item: 
          Title: Gene ontology analysis
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       Item: 
        Title: Results
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         Item: 
          Title: Study and experimental design
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         Item: 
          Title: Clinical features of the patient’s cohort
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         Item: 
          Title: Clinical laboratory data
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         Item: 
          Title: Humoral immune response
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         Item: 
          Title: Isolation, characterization and proteomic analysis of circulating EVs during active COVID-19 infection
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         Item: 
          Title: Molecular EV-signatures in COVID-19 patients with different clinical manifestations
          Destination: s3_6
         Item: 
          Title: Molecular signatures of COVID-19 progression
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       Item: 
        Title: Discussion
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       Item: 
        Title: Data availability statement
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       Item: 
        Title: Ethics statement
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       Item: 
        Title: Author contributions
        Destination: bmark_-1208584890
       Item: 
        Title: Funding
        Destination: bmark_1865464027
       Item: 
        Title: Acknowledgments
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       Item: 
        Title: Conflict of interest
        Destination: bmark_1089982005
       Item: 
        Title: Publisher’s note
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       Item: 
        Title: Author disclaimer
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       Item: 
        Title: Supplementary material
        Destination: bmark_632582120
       Item: 
        Title: References
        Destination: bmark_2090415954
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    Title: Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides
    Author: Melisa Gualdrón-López
    Subject: Gualdrón-López M, Ayllon-Hermida A, Cortes-Serra N, Resa-Infante P, Bech-Serra JJ, Aparici-Herraiz I, Nicolau-Fernandez M, Erkizia I, Gutierrez-Chamorro L, Marfil S, Pradenas E, Ávila Nieto C, Cucurull B, Montaner-Tarbés S, Muelas M, Sotil R, Ballana E, Urrea V, Fraile L, Montoya M, Vergara J, Segales J, Carrillo J, Izquierdo-Useros N, Blanco J, Fernandez-Becerra C, de La Torre C, Pinazo M-J, Martinez-Picado J and del Portillo HA (2024) Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides. Front. Cell. Infect. Microbiol. 14:1442743. doi: 10.3389/fcimb.2024.1442743
    Keywords: COVID-19 patients; SARS-CoV-2; antibody response; extracellular vesicles; immunocapture (CD9); ganglioside-capture (CD169/Siglec-1); size-exclusion chromatography (SEC); proteomics profiling
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               <rdf:li xml:lang="x-default">Gualdrón-López M, Ayllon-Hermida A, Cortes-Serra N, Resa-Infante P, Bech-Serra JJ, Aparici-Herraiz I, Nicolau-Fernandez M, Erkizia I, Gutierrez-Chamorro L, Marfil S, Pradenas E, Ávila Nieto C, Cucurull B, Montaner-Tarbés S, Muelas M, Sotil R, Ballana E, Urrea V, Fraile L, Montoya M, Vergara J, Segales J, Carrillo J, Izquierdo-Useros N, Blanco J, Fernandez-Becerra C, de La Torre C, Pinazo M-J, Martinez-Picado J and del Portillo HA (2024) Proteomics of circulating extracellular vesicles reveals diverse clinical presentations of COVID-19 but fails to identify viral peptides. Front. Cell. Infect. Microbiol. 14:1442743. doi: 10.3389/fcimb.2024.1442743</rdf:li>
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         <dc:rights>
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               <rdf:li xml:lang="x-default">Copyright © 2024 Gualdrón-López, Ayllon-Hermida, Cortes-Serra, Resa-Infante, Bech-Serra, Aparici-Herraiz, Nicolau-Fernandez, Erkizia, Gutierrez-Chamorro, Marfil, Pradenas, Ávila Nieto, Cucurull, Montaner-Tarbés, Muelas, Sotil, Ballana, Urrea, Fraile, Montoya, Vergara, Segales, Carrillo, Izquierdo-Useros, Blanco, Fernandez-Becerra, de La Torre, Pinazo, Martinez-Picado and del Portillo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</rdf:li>
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   Type: CRC32
  Checksum: cc22e34e149930b177eb5fa5cc332532
   Type: MD5
  Checksum: 07ed12191045b3fe99911e57a7812d66bde3eefd
   Type: SHA-1
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   Type: SHA-256