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Title: Assessment of brain-derived extracellular vesicle enrichment for blood biomarker analysis in age-related neurodegenerative diseases: An international overview Destination: chapter..1 Children: Item: Title: Abstract Destination: section*.2 Item: Title: 1 | INTRODUCTION Destination: section..1.1 Item: Title: 2 | METHODS Destination: section..1.2 Children: Item: Title: 2.1 | Literature search Destination: subsection..1.2.1 Item: Title: 2.2 | Data extraction Destination: subsection..1.2.2 Item: Title: 2.3 | Characterization of proteins in our list Destination: subsection..1.2.3 Item: Title: 3 | RESULTS Destination: section..1.3 Children: Item: Title: 3.1 | Literature search and identification of IP-based BEV articles Destination: subsection..1.3.1 Item: Title: 3.2 | Assessment of sample preparation, EV, and IP-based BEV enrichment protocols Destination: subsection..1.3.2 Children: Item: Title: 3.2.1 | Sample preparation for downstream enrichment of BEVs Destination: subsubsection..1.3.2.1 Item: Title: 3.2.2 | EV enrichment Destination: subsubsection..1.3.2.2 Item: Title: 3.2.3 | IP-based BEV-enrichment from blood Destination: subsubsection..1.3.2.3 Item: Title: 3.3 | EV and BEV characterization by size, shape, and cellular origin Destination: subsection..1.3.3 Item: Title: 3.4 | CNS-specificity, ECD status, and presence in public EV databases of proteins in our list Destination: subsection..1.3.4 Children: Item: Title: 3.4.1 | Brain cell-type-specificity of proteins in our list identified as CNS-specific Destination: subsubsection..1.3.4.1 Item: Title: 4 | DISCUSSION Destination: section..1.4 Children: Item: Title: 4.1 | Commonly used experimental choices across NDD studies Destination: subsection..1.4.1 Children: Item: Title: 4.1.1 | Biophysical characterization of EVs based on size and shape Destination: subsubsection..1.4.1.1 Item: Title: 4.1.2 | Choice of plasma over serum as starting material Destination: subsubsection..1.4.1.2 Item: Title: 4.1.3 | Choice of thromboplastin-D over thrombin for defibrination Destination: subsubsection..1.4.1.3 Item: Title: 4.1.4 | Choice of ExoQuick over other approaches for EV enrichment Destination: subsubsection..1.4.1.4 Item: Title: 4.1.5 | Choice of L1CAM for BEV enrichment Destination: subsubsection..1.4.1.5 Item: Title: 4.2 | Recommendations for CNS-specific alternatives to L1CAM for NEV enrichment from blood Destination: subsection..1.4.2 Item: Title: 4.3 | CONCLUSIONS Destination: subsection..1.4.3 Item: Title: AUTHOR CONTRIBUTIONS Destination: section*.4 Item: Title: ACKNOWLEDGMENTS Destination: section*.6 Item: Title: CONFLICT OF INTEREST STATEMENT Destination: section*.8 Children: Item: Title: DATA AVAILABILITY STATEMENT Destination: section*.10 Item: Title: CONSENT STATEMENT Destination: section*.12 Item: Title: REFERENCES Destination: section*.14 Item: Title: SUPPORTING INFORMATION Destination: section*.16 Item: Title: APPENDIX Destination: appendix..A Children: Item: Title: COLLABORATORS-BBB PIA Exosome Working Group (EWG), Established October 2021 Destination: section*.18 Info: Title: Assessment of brain‐derived extracellular vesicle enrichment for blood biomarker analysis in age‐related neurodegenerative diseases: An international overview Author: Subject: Keywords: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 10.0.0 (Windows) CreationDate: Tue Jul 02 15:19:29 CEST 2024 ModDate: Tue Jul 02 15:19:57 CEST 2024 ID: 0x851f5690ebe3c7879366773da9386fdf, 0xcabce7c89167222ce8971bbe6c867a2a Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 2100 ImageHeight: 1246 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2100 ImageHeight: 1528 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg 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