835293202253dc3278ce59d2cb9a28a9 pets-01-00033.pdf 2c8910c7f36acd3cfff3443f23dbde6e81b9b603 pets-01-00033.pdf 908ff246e9f1cfa5dd7248a513520e4b16ab3fbb3fd492afd1e55eb1bf3a2c67 pets-01-00033.pdf Title: Dietary Inulin Supplementation Modulates Fecal Microbiota and Vaccine Response in Cats—A Double-Blind Randomized Controlled Pilot Study Subject: Twenty-six cats were fed either a control or an inulin-supplemented diet (0.6%) for six weeks with measurements of fecal short-chain fatty acids (SCFAs) and microbiota. In the second phase, cats were fed their respective diets for another six weeks and were then primo-vaccinated against the feline leukemia virus (FeLV) at days 0 and 21. Serum anti-FeLV IgG responses were monitored. Compared to the control group, the inulin group exhibited higher Firmicutes (+14%) and lower Bacteroidetes (-56%) and Prevotellaceae (-51%) after six weeks of supplementation. Compared to T0, SCFAs initially decreased at week 3 but subsequently increased at week 6 with inulin supplementation, leading to higher butyrate compared to the control group at week 6. A significant diet-by-time interaction was also observed for propionate and total SCFAs. Compared to the control group, the inulin group tended to show a higher serum anti-FeLV IgG response after the first vaccination (p = 0.09), with significant differences at days 5 and 11 post-vaccination. These preliminary results suggest that a six-week dietary supplementation with a low dose of inulin in cats can modify the fecal microbiota and its functional metabolites, potentially influencing the early immune response to vaccination. Keywords: inulin; fecal microbiota; prebiotic; immune response; fructans; short-chain fatty acids Author: Isabelle C. Jeusette, Emmanuelle Apper, Marina L. Meli, Regina Hofmann-Lehmann, Anna Salas-Mani, Anna Fernández-Pinteño, Olga Francino and Celina Torre Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Dec 20 15:25:30 2024 CET ModDate: Fri Dec 20 15:28:02 2024 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2019195 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 VPCSMT+URWPalladioL-Bold Type 1 Custom yes yes yes 16 0 UFFUPH+URWPalladioL-Roma Type 1 Custom yes yes yes 22 0 BNHPLZ+URWPalladioL-Ital Type 1 Custom yes yes yes 27 0 FRRFFK+CMSY10 Type 1 Builtin yes yes yes 32 0 MWHIBA+VnURWPalladioL Type 1 Custom yes yes yes 37 0 VVSYYB+EURM10 Type 1 Builtin yes yes yes 42 0 IIABFN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 82 0 IIABFO+PalatinoLinotype CID TrueType Identity-H yes yes yes 85 0 IIABGP+PalatinoLinotype TrueType WinAnsi yes yes no 91 0 IIABHB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 94 0 IIABNP+PalatinoLinotype TrueType MacRoman yes yes no 97 0 IIABFN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 114 0 IIABFO+PalatinoLinotype CID TrueType Identity-H yes yes yes 117 0 IIABGP+PalatinoLinotype TrueType WinAnsi yes yes no 123 0 IIABHB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 126 0 IIABNP+PalatinoLinotype TrueType MacRoman yes yes no 129 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 139 0 IIAFBA+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 154 0 IIABFN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 157 0 IIABFO+PalatinoLinotype CID TrueType Identity-H yes yes yes 160 0 IIABGP+PalatinoLinotype TrueType WinAnsi yes yes no 166 0 IIABHB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 169 0 IIABNP+PalatinoLinotype TrueType MacRoman yes yes no 172 0 IIAFBA+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 184 0 IIABFN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 187 0 IIABFO+PalatinoLinotype CID TrueType Identity-H yes yes yes 190 0 IIABGP+PalatinoLinotype TrueType WinAnsi yes yes no 196 0 IIABHB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 199 0 IIABNP+PalatinoLinotype TrueType MacRoman yes yes no 202 0 IIABFN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 221 0 IIABFO+PalatinoLinotype CID TrueType Identity-H yes yes yes 224 0 IIABGP+PalatinoLinotype TrueType WinAnsi yes yes no 230 0 IIABHB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 233 0 IIABNP+PalatinoLinotype TrueType MacRoman yes yes no 236 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-21 02:10:22 CET RepresentationInformation: pets-01-00033.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-20 15:01:17 CET Size: 2019195 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 453 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: Study Part 1: Short-Chain Fatty Acids, CD4+, CD8+, Microbiota Destination: subsection.2.2 Item: Title: Study Part 2: Vaccine Challenge and Specific Immune Response Destination: subsection.2.3 Item: Title: Statistics Destination: subsection.2.4 Item: Title: Ethical Approval Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: SCFAs, CD4+, CD8+, and Fecal Microbiota (Part 1) Destination: subsection.3.1 Item: Title: Vaccine Response (Part 2) Destination: subsection.3.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: appendix.A. Info: Title: Dietary Inulin Supplementation Modulates Fecal Microbiota and Vaccine Response in Cats A Double-Blind Randomized Controlled Pilot Study Author: Isabelle C. Jeusette, Emmanuelle Apper, Marina L. Meli, Regina Hofmann-Lehmann, Anna Salas-Mani, Anna Fernández-Pinteño, Olga Francino and Celina Torre Subject: Twenty-six cats were fed either a control or an inulin-supplemented diet (0.6%) for six weeks with measurements of fecal short-chain fatty acids (SCFAs) and microbiota. In the second phase, cats were fed their respective diets for another six weeks and were then primo-vaccinated against the feline leukemia virus (FeLV) at days 0 and 21. Serum anti-FeLV IgG responses were monitored. Compared to the control group, the inulin group exhibited higher Firmicutes (+14%) and lower Bacteroidetes (-56%) and Prevotellaceae (-51%) after six weeks of supplementation. Compared to T0, SCFAs initially decreased at week 3 but subsequently increased at week 6 with inulin supplementation, leading to higher butyrate compared to the control group at week 6. A significant diet-by-time interaction was also observed for propionate and total SCFAs. Compared to the control group, the inulin group tended to show a higher serum anti-FeLV IgG response after the first vaccination (p = 0.09), with significant differences at days 5 and 11 post-vaccination. These preliminary results suggest that a six-week dietary supplementation with a low dose of inulin in cats can modify the fecal microbiota and its functional metabolites, potentially influencing the early immune response to vaccination. 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