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Title:           Pericardium decellularization in a one-day, two-step protocol
Subject:         Molecular and Cellular Biochemistry, https://doi.org/10.1007/s11010-024-05086-x
Keywords:        Decellularization; Pericardial allograft; Human acellular matrix; Tissue establishment; Tissue engineering
Author:          P. López-Chicón 
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      Title: Pericardium decellularization in a one-day, two-step protocol
      Children: 
       Item: 
        Title: Abstract
       Item: 
        Title: Introduction
       Item: 
        Title: Materials and methods
        Children: 
         Item: 
          Title: Ethical considerations
         Item: 
          Title: Pericardium procurement
         Item: 
          Title: Reagents
         Item: 
          Title: Decellularization protocols
         Item: 
          Title: Microbiological assessment
         Item: 
          Title: Analysis of the extracellular matrix
          Children: 
           Item: 
            Title: Qualitative assay: histology and structure
         Item: 
          Title: Quantitative assays: ECM biomolecule analysis
         Item: 
          Title: DNA evaluation
         Item: 
          Title: Cell viability
          Children: 
           Item: 
            Title: Biomechanical testing by uniaxial mechanical assay
           Item: 
            Title: Residual glycerol quantification
         Item: 
          Title: Statistical analysis
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Histological and macroscopic evaluation of DPM
         Item: 
          Title: Microbiological assessment
         Item: 
          Title: DNA quantification
          Children: 
           Item: 
            Title: Structural ECM biomolecule content
           Item: 
            Title: Biomechanical properties
           Item: 
            Title: Residual glycerol quantification
         Item: 
          Title: Cell viability
       Item: 
        Title: Discussion
       Item: 
        Title: Conclusion
       Item: 
        Title: Acknowledgements 
       Item: 
        Title: References
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   Type: CRC32
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   Type: MD5
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   Type: SHA-1
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   Type: SHA-256