97cbaae03f01d5ddda64304a25516e3f 309003.pdf 061218d962e03170c197a32a6a2f2d5d3ecc129d 309003.pdf 5530b6472326c528a094b0fd255732c27ab14a1ab5c9cbf59e6b2e6f6cf67e6f 309003.pdf Title: Characterization of Immune Cell Populations and Acid-Sensing Receptors in the Human Esophagus Subject: Introduction: Esophageal inflammatory diseases are frequent diagnoses in clinical practice and have diverse etiologies, the most common being those associated with the exposure to gastric content, drugs and allergens. In diseases, the immunological component is well identified in endoscopic biopsies, which mainly contain the epithelium and the lamina propria; however, deeper layers are less studied. Moreover, the esophageal capacity of sensing luminal compounds is poorly understood. Methods: In transmural sections from proximal, middle and distal esophagus obtained from deceased patients, we performed a phenotypic analysis of the main immune cell populations and acid-sensing receptors by immunohistochemistry and immunofluorescence methods. Results: A total of nine donors were studied (absence of pathology, optimal tissue preservation and orientation). We found the following: (1) the vascular papillae and the lamina propria are the most infiltrated layers by the lymphoid lineage (T and B lymphocytes), followed by the epithelium, while the smooth muscular layers are mainly populated by the myeloid lineage (macrophages and mast cells); (2) intraepithelial macrophages are consistently found along the esophagus; and (3) eosinophils are absent in all the esophageal layers. The acid-sensing receptors ASIC-1, ASIC-2 and ENAC are expressed in the esophageal epithelium and in the lamina propria, yet only ASIC-2 is expressed in the muscularis mucosae. Conclusions: The human esophagus contains a differential distribution of immune cells and acid-sensing receptors across its layers. This study extends the esophageal histological knowledge previously described and reinforces its role as a defensive and sensing organ. Keywords: esophagus; immune system; acid-sensing receptors Author: Marina Fortea, Leen Hacour, Francesc Sancho, Carlos Boada, Cesar Sevillano-Aguilera, Ana María. González-Castro, Eloisa Salvo-Romero, Beatriz Lobo, Danila Guagnozzi, Laurens J. Ceulemans, Jacques Pirenne, Carmen Alonso-Cotoner, Javier Santos, Ricard Farré and Maria Vicario Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25 CreationDate: Fri Sep 20 09:30:41 2024 CEST ModDate: Fri Sep 20 09:37:02 2024 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4932074 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes yes 10 0 NKXLQD+EURM10 Type 1 Builtin yes yes yes 16 0 OQVACK+URWPalladioL-Bold Type 1 Custom yes yes yes 21 0 BNBIKU+URWPalladioL-Roma Type 1 Custom yes yes yes 27 0 BWHAZV+URWPalladioL-Ital Type 1 Custom yes yes yes 32 0 CIQLLF+VnURWPalladioL Type 1 Custom yes yes yes 37 0 QYNZBG+CMSY10 Type 1 Builtin yes yes yes 76 0 XATTIN+PazoMath-Italic Type 1 Builtin yes yes yes 84 0 QQXUPP+PazoMath Type 1 Builtin yes yes yes 89 0 CZKGAS+CMR10 Type 1 Builtin yes yes yes 94 0 AFONKM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 109 0 AFONKN+PalatinoLinotype CID TrueType Identity-H yes yes yes 112 0 AFONKO+PalatinoLinotype TrueType WinAnsi yes yes no 118 0 AFONLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 121 0 AFOOKN+PalatinoLinotype TrueType MacRoman yes yes no 124 0 AFONKM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 138 0 AFONKN+PalatinoLinotype CID TrueType Identity-H yes yes yes 141 0 AFONKO+PalatinoLinotype TrueType WinAnsi yes yes no 147 0 AFONLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 150 0 AFOOKN+PalatinoLinotype TrueType MacRoman yes yes no 153 0 KPQTSC+URWPalladioL-BoldItal Type 1 Custom yes yes yes 161 0 AFONKM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 176 0 AFONKO+PalatinoLinotype TrueType WinAnsi yes yes no 179 0 AFONLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 182 0 AFOOKN+PalatinoLinotype TrueType MacRoman yes yes no 185 0 AFONKM+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 199 0 AFONKN+PalatinoLinotype CID TrueType Identity-H yes yes yes 202 0 AFONKO+PalatinoLinotype TrueType WinAnsi yes yes no 208 0 AFONLA+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 211 0 AFOOKN+PalatinoLinotype TrueType MacRoman yes yes no 214 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-03-15 02:43:05 CET RepresentationInformation: 309003.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-03-14 13:29:11 CET Size: 4932074 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 444 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Material and Methods Destination: section.2 Children: Item: Title: Biological Samples Destination: subsection.2.1 Item: Title: Tissue Collection Destination: subsection.2.2 Item: Title: Histological Procedures Destination: subsection.2.3 Children: Item: Title: Immunohistochemistry Destination: subsubsection.2.3.1 Item: Title: Immunofluorescence Destination: subsubsection.2.3.2 Item: Title: Quantitative Analysis of Immune Cells Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Study Population Destination: subsection.3.1 Item: Title: Structural Analysis Destination: subsection.3.2 Item: Title: Distribution and Phenotype of Immune Cells in the Esophagus Destination: subsection.3.3 Item: Title: Distribution of Acid-Sensing Receptors in the Esophagus Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: appendix.A. Info: Title: Characterization of Immune Cell Populations and Acid-Sensing Receptors in the Human Esophagus Author: Marina Fortea, Leen Hacour, Francesc Sancho, Carlos Boada, Cesar Sevillano-Aguilera, Ana María. González-Castro, Eloisa Salvo-Romero, Beatriz Lobo, Danila Guagnozzi, Laurens J. Ceulemans, Jacques Pirenne, Carmen Alonso-Cotoner, Javier Santos, Ricard Farré and Maria Vicario Subject: ぉのぴひはつふっぴどはの〺〠ぅびはばとちでづちぬ〠どのてぬちねねちぴはひべ〠つどびづちびづび〠ちひづ〠てひづぱふづのぴ〠つどちでのはびづび〠どの〠っぬどのどっちぬ〠ばひちっぴどっづ〠ちのつ〠とちぶづ〠つどぶづひびづ〠づぴどはぬはでどづび〬〠ぴとづ〠ねはびぴ〠っはねねはの〠ぢづどので〠ぴとはびづ〠ちびびはっどちぴづつ〠ぷどぴと〠ぴとづ〠づへばはびふひづ〠ぴは〠でちびぴひどっ〠っはのぴづのぴ〬〠つひふでび〠ちのつ〠ちぬぬづひでづのび〮〠ぉの〠つどびづちびづび〬〠ぴとづ〠どねねふのはぬはでどっちぬ〠っはねばはのづのぴ〠どび〠ぷづぬぬ〠どつづのぴどてどづつ〠どの〠づのつはびっはばどっ〠ぢどはばびどづび〬〠ぷとどっと〠ねちどのぬべ〠っはのぴちどの〠ぴとづ〠づばどぴとづぬどふね〠ちのつ〠ぴとづ〠ぬちねどのち〠ばひはばひどち〻〠とはぷづぶづひ〬〠つづづばづひ〠ぬちべづひび〠ちひづ〠ぬづびび〠びぴふつどづつ〮〠きはひづはぶづひ〬〠ぴとづ〠づびはばとちでづちぬ〠っちばちっどぴべ〠はて〠びづのびどので〠ぬふねどのちぬ〠っはねばはふのつび〠どび〠ばははひぬべ〠ふのつづひびぴははつ〮〠きづぴとはつび〺〠ぉの〠ぴひちのびねふひちぬ〠びづっぴどはのび〠てひはね〠ばひはへどねちぬ〬〠ねどつつぬづ〠ちのつ〠つどびぴちぬ〠づびはばとちでふび〠はぢぴちどのづつ〠てひはね〠つづっづちびづつ〠ばちぴどづのぴび〬〠ぷづ〠ばづひてはひねづつ〠ち〠ばとづのはぴべばどっ〠ちのちぬべびどび〠はて〠ぴとづ〠ねちどの〠どねねふのづ〠っづぬぬ〠ばはばふぬちぴどはのび〠ちのつ〠ちっどつ〭びづのびどので〠ひづっづばぴはひび〠ぢべ〠どねねふのはとどびぴはっとづねどびぴひべ〠ちのつ〠どねねふのはてぬふはひづびっづのっづ〠ねづぴとはつび〮〠げづびふぬぴび〺〠ぁ〠ぴはぴちぬ〠はて〠のどのづ〠つはのはひび〠ぷづひづ〠びぴふつどづつ〠〨ちぢびづのっづ〠はて〠ばちぴとはぬはでべ〬〠はばぴどねちぬ〠ぴどびびふづ〠ばひづびづひぶちぴどはの〠ちのつ〠はひどづのぴちぴどはの〩〮〠しづ〠てはふのつ〠ぴとづ〠てはぬぬはぷどので〺〠〨〱〩〠ぴとづ〠ぶちびっふぬちひ〠ばちばどぬぬちづ〠ちのつ〠ぴとづ〠ぬちねどのち゠ばひはばひどち〠ちひづ〠ぴとづ〠ねはびぴ〠どのてどぬぴひちぴづつ〠ぬちべづひび〠ぢべ〠ぴとづ〠ぬべねばとはどつ〠ぬどのづちでづ〠〨ご〠ちのつ〠あ〠ぬべねばとはっべぴづび〩〬〠てはぬぬはぷづつ〠ぢべ〠ぴとづ〠づばどぴとづぬどふね〬〠ぷとどぬづ〠ぴとづ〠びねははぴと〠ねふびっふぬちひ〠ぬちべづひび〠ちひづ〠ねちどのぬべ〠ばはばふぬちぴづつ〠ぢべ〠ぴとづ〠ねべづぬはどつ〠ぬどのづちでづ〠〨ねちっひはばとちでづび〠ちのつ〠ねちびぴ〠っづぬぬび〩〻〠〨〲〩〠どのぴひちづばどぴとづぬどちぬ〠ねちっひはばとちでづび〠ちひづ〠っはのびどびぴづのぴぬべ〠てはふのつ〠ちぬはので〠ぴとづ〠づびはばとちでふび〻〠ちのつ〠〨〳〩〠づはびどのはばとどぬび〠ちひづ〠ちぢびづのぴ〠どの〠ちぬぬ〠ぴとづ〠づびはばとちでづちぬ〠ぬちべづひび〮〠ごとづ〠ちっどつ〭びづのびどので〠ひづっづばぴはひび〠ぁこぉぃ〭〱〬〠ぁこぉぃ〭〲〠ちのつ〠ぅぎぁぃ〠ちひづ〠づへばひづびびづつ〠どの〠ぴとづ〠づびはばとちでづちぬ〠づばどぴとづぬどふね〠ちのつ〠どの〠ぴとづ〠ぬちねどのち〠ばひはばひどち〬〠べづぴ〠はのぬべ〠ぁこぉぃ〭〲〠どび〠づへばひづびびづつ〠どの〠ぴとづ〠ねふびっふぬちひどび〠ねふっはびちづ〮〠ぃはのっぬふびどはのび〺〠ごとづ〠とふねちの〠づびはばとちでふび〠っはのぴちどのび〠ち〠つどててづひづのぴどちぬ〠つどびぴひどぢふぴどはの〠はて〠どねねふのづ〠っづぬぬび〠ちのつ〠ちっどつ〭びづのびどので〠ひづっづばぴはひび〠ちっひはびび〠どぴび〠ぬちべづひび〮〠ごとどび〠びぴふつべ〠づへぴづのつび〠ぴとづ〠づびはばとちでづちぬ〠とどびぴはぬはでどっちぬ〠にのはぷぬづつでづ〠ばひづぶどはふびぬべ〠つづびっひどぢづつ〠ちのつ〠ひづどのてはひっづび〠どぴび〠ひはぬづ〠ちび〠ち〠つづてづのびどぶづ〠ちのつ〠びづのびどので〠はひでちの〮 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