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PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: The interferon γ pathway enhances pluripotency and X-chromosome reactivation in iPSC reprogramming Children: Item: Title: INTRODUCTION Item: Title: RESULTS Children: Item: Title: A genome-wide CRISPR KO screen reveals molecular networks involved in reprogramming and X-chromosome reactivation Item: Title: IFNγ signaling modulates colony formation and X-chromosome reactivation during iPSC reprogramming Item: Title: IFNγ pathway activation accelerates the reprogramming process Item: Title: IFNγ treatment during reprogramming enhances JAK-STAT3 signaling, pluripotency gene expression, and X-chromosome reactivation Item: Title: IFNγ treatment promotes TET-mediated DNA demethylation in cells undergoing reprogramming Item: Title: DISCUSSION Children: Item: Title: Limitations of the study Item: Title: MATERIALS AND METHODS Children: Item: Title: Cell lines used Children: Item: Title: PaX cell line Item: Title: PaX-iCas9 cell line Item: Title: Stat1−/−, Irf1−/−, Tet1−/−, and Stat3−/− cell lines Item: Title: Stat3-BFP overexpression ESC pools Item: Title: Primary cell derivation and animal husbandry Item: Title: Feeders (irradiated MEFs) Item: Title: ESC culture Item: Title: NPC differentiation Item: Title: Reprogramming of NPCs into iPSCs Item: Title: Lentiviral CRISPR KO screen Children: Item: Title: gRNA library amplification Item: Title: Generation of lentiviral gRNA library Item: Title: gRNA library lentiviral infection of ESCs Item: Title: NPC differentiation, reprogramming, and cell isolation by FACS Item: Title: Sample preparation and gRNA sequencing Item: Title: CRISPR screening analysis Item: Title: CRISPR screening pathway validation—molecule screening Item: Title: Reprogramming of MEFs into iPSCs Item: Title: Flow cytometry Item: Title: IFNγ treatment during NPC to iPSC reprogramming Item: Title: IFNγ treatment during NPC differentiation Item: Title: RNA isolation, cDNA synthesis, and qRT-PCR Item: Title: DNA methylation modifier experiments Item: Title: Apoptosis assay Item: Title: Western blotting Item: Title: AP staining Item: Title: Immunofluorescence Item: Title: RNA FISH Item: Title: X-chromosome paint Item: Title: RNA-seq experiments Item: Title: RNA-seq analysis Item: Title: DNA (hydroxy)methylation experiments and analyses Item: Title: Allele-specific (hydroxy)methylation analysis by amplicon oxidative BS sequencing Item: Title: Statistical analyses Item: Title: Supplementary Materials Children: Item: Title: This PDF file includes: Item: Title: Other Supplementary Material for this manuscript includes the following: Item: Title: REFERENCES AND NOTES Item: Title: Acknowledgments Info: Title: Science Journals   AAAS Creator: Adobe InDesign 16.4 (Windows) Producer: Adobe PDF Library 16.0 CreationDate: Mon Jul 29 02:50:54 CEST 2024 ModDate: Wed Aug 07 13:26:18 CEST 2024 ID: 0x58a70aa19d9d7a4d908a54392ebbc0fd, 0x95754a5bb85fa64784766b04dc46b869 Filters: 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ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 63 ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 59 ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 58 ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 195 ImageHeight: 195 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 62 ImageHeight: 61 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 26 ImageHeight: 26 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 114 ImageHeight: 91 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 381 ImageHeight: 151 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 58 ImageHeight: 57 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 361 ImageHeight: 355 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 423 ImageHeight: 134 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 483 ImageHeight: 54 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 353 ImageHeight: 356 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 483 ImageHeight: 63 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 483 ImageHeight: 85 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 255 ImageHeight: 255 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 256 ImageHeight: 255 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 256 ImageHeight: 255 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 255 ImageHeight: 255 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 46 ImageHeight: 128 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 152 ImageHeight: 341 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 414 ImageHeight: 549 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 273 ImageHeight: 224 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 162 ImageHeight: 69 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 122 ImageHeight: 115 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 201 ImageHeight: 69 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 499 ImageHeight: 418 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 17 ImageHeight: 49 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 300 ImageHeight: 260 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 596 ImageHeight: 69 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 28 ImageHeight: 29 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 200 ImageHeight: 69 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 530 ImageHeight: 63 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 332 ImageHeight: 79 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 596 ImageHeight: 201 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 201 ImageHeight: 34 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 201 ImageHeight: 62 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 184 ImageHeight: 198 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 200 ImageHeight: 59 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 594 ImageHeight: 191 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 592 ImageHeight: 224 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 594 ImageHeight: 67 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 531 ImageHeight: 179 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 101 ImageHeight: 99 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 200 ImageHeight: 68 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 578 ImageHeight: 100 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 75 ImageHeight: 4 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 209 ImageHeight: 208 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 75 ImageHeight: 136 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 19 ImageHeight: 53 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 10 ImageHeight: 89 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 75 ImageHeight: 69 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 152 ImageHeight: 93 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 152 ImageHeight: 224 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 52 ImageHeight: 116 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 52 ImageHeight: 116 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 50 ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 14 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 359 ImageHeight: 244 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 50 ImageHeight: 50 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 14 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 47 ImageHeight: 8 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 12 ImageHeight: 4 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 46 ImageHeight: 21 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 4 ImageHeight: 18 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 86 ImageHeight: 8 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 12 ImageHeight: 4 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 5 ImageHeight: 8 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 18 ImageHeight: 24 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 40 ImageHeight: 28 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 6 ImageHeight: 27 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 44 ImageHeight: 60 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 51 ImageHeight: 26 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 95 ImageHeight: 60 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 207 ImageHeight: 203 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 6 ImageHeight: 7 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 44 ImageHeight: 30 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 95 ImageHeight: 30 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 55 ImageHeight: 79 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 103 ImageHeight: 130 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 57 ImageHeight: 131 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 101 ImageHeight: 81 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 20 ImageHeight: 26 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Decode: 0, 255 Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 209 ImageHeight: 133 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 55 ImageHeight: 53 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 101 ImageHeight: 53 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 77 ImageHeight: 54 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 111 ImageHeight: 109 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 595 ImageHeight: 109 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 247 ImageHeight: 109 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 13 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 77 ImageHeight: 21 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 7 ImageHeight: 20 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 15 ImageHeight: 6 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 12 ImageHeight: 5 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 3 ImageHeight: 18 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: IRVRES+ArialMT Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: MXTPIK+Arial-ItalicMT FontSubset: true FirstChar: 49 LastChar: 116 FontDescriptor: FontName: MXTPIK+Arial-ItalicMT Flags: Nonsymbolic, Italic FontBBox: -262, -210, 1062, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: MXTPIK+Arial-ItalicMT FontSubset: true FirstChar: 32 LastChar: 80 FontDescriptor: FontName: MXTPIK+Arial-ItalicMT Flags: Nonsymbolic, Italic FontBBox: -262, -210, 1062, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: JQFXSQ+Helvetica FontSubset: true FirstChar: 31 LastChar: 116 FontDescriptor: FontName: JQFXSQ+Helvetica Flags: Nonsymbolic FontBBox: -166, -225, 1000, 931 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: DHINMC+Helvetica-Oblique FontSubset: true FirstChar: 90 LastChar: 90 FontDescriptor: FontName: DHINMC+Helvetica-Oblique Flags: Nonsymbolic, Italic FontBBox: -933, -481, 1571, 1138 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 29 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 122 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: MXTPIK+Arial-ItalicMT FontSubset: true FirstChar: 49 LastChar: 116 FontDescriptor: FontName: MXTPIK+Arial-ItalicMT Flags: Nonsymbolic, Italic FontBBox: -262, -210, 1062, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 30 LastChar: 122 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: JQFXSQ+Helvetica FontSubset: true FirstChar: 46 LastChar: 56 FontDescriptor: FontName: JQFXSQ+Helvetica Flags: Nonsymbolic FontBBox: -166, -225, 1000, 931 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: MXTPIK+Arial-ItalicMT FontSubset: true FirstChar: 32 LastChar: 110 FontDescriptor: FontName: MXTPIK+Arial-ItalicMT Flags: Nonsymbolic, Italic FontBBox: -262, -210, 1062, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: JQFXSQ+Helvetica FontSubset: true FirstChar: 45 LastChar: 52 FontDescriptor: FontName: JQFXSQ+Helvetica Flags: Nonsymbolic FontBBox: -166, -225, 1000, 931 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 31 LastChar: 121 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 65 LastChar: 120 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial-BoldMT FontSubset: true FirstChar: 52 LastChar: 120 FontDescriptor: FontName: OASBKG+Arial-BoldMT Flags: Nonsymbolic FontBBox: -167, -210, 1000, 913 FontFile3: true Encoding: WinAnsiEncoding Font: BaseFont: OASBKG+Arial FontSubset: true FirstChar: 30 LastChar: 122 FontDescriptor: FontName: OASBKG+Arial Flags: Nonsymbolic FontBBox: -665, -325, 2000, 1006 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true Font: BaseFont: 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